公开(公告)号：US20230049760A1

公开(公告)日：2023-02-16

申请号：US17789598

申请日：2020-12-18

Applicant: DSM IP ASSETS B.V.

Inventor： Jenna MCMAHON ,  Elvin Irsan KOOI ,  Liang WU ,  René Marcel DE JONG ,  Valmik Kanubhai VYAS ,  Peter Louis HOUSTON

IPC: C12N9/20 ,  C12P23/00 ,  C12Q1/44

Abstract: The present invention is related to a retinoid-producing host cell, particularly oleaginous yeast, modified such that the percentage of retinyl acetate based on the total retinoids produced by such host cell is increased during fermentation using triglyceride oils, like for example vegetable oil, as carbon source, wherein the activity of certain endogenous hydrolases or transferases involved in undesired conversions of retinol or retinol acetate is reduced or abolished. Particularly, such modified host cell might be useful in a biotechnological process for production of vitamin A.

公开(公告)号：US20240279706A1

公开(公告)日：2024-08-22

申请号：US18292227

申请日：2021-07-27

Applicant: DSM IP ASSETS B.V.

Inventor： Peter Louis HOUSTON ,  Valmik Kanubhai VYAS ,  Anna SYMBOR-NAGRABSKA ,  Reed Chadbourne DOTEN ,  Christopher Mark FARRELL ,  Ethan LAM

IPC: C12P23/00 ,  C12N1/16 ,  C12N9/10

CPC classification number: C12P23/00 ,  C12N1/16 ,  C12N9/1029

Abstract: The present invention is related to a novel process for production of retinyl acetate in a host cell, particularly oleaginous yeast such as e.g. Yarrowia, wherein the product purity could be increased with reduction of unwanted side-products. Particularly, the novel process comprises fermentation in the presence of ethanol, such as e.g. in a fed-batch fermentation process. Such process is especially useful in a biotechnological process for production of vitamin A.

公开(公告)号：US20230265443A1

公开(公告)日：2023-08-24

申请号：US18017819

申请日：2021-07-22

Applicant: DSM IP ASSETS B.V.

Inventor： Peter Louis HOUSTON ,  Valmik Kanubhai VYAS ,  René Marcel DE JONG ,  Anna SYMBOR-NAGRABSKA ,  Ethan LAM

IPC: C12N15/81 ,  C12N1/16 ,  C12P23/00 ,  C12N9/20

CPC classification number: C12N15/815 ,  C12N1/16 ,  C12P23/00 ,  C12N9/20 ,  C12N2500/36 ,  C12Y301/01003

Abstract: The present invention is related to a novel process for production of retinyl acetate in a host cell, particularly oleaginous yeast such as e.g. Yarrowia, growing on triglyceride oils, such as e.g. vegetable oil, wherein the host cell exhibits modified lipase activity in such a way that conversion of retinol into fatty acid retinyl esters (FAREs) is reduced or abolished. Such process is especially useful in a biotechnological process for production of vitamin A.

公开(公告)号：US20250002873A1

公开(公告)日：2025-01-02

申请号：US18700931

申请日：2022-10-19

Applicant: DSM IP ASSETS B.V.

Inventor： Valmik Kanubhai VYAS ,  Peter Louis HOUSTON ,  Jenna McMAHON ,  René Marcel DE JONG

IPC: C12N9/04 ,  C12P7/22

Abstract: The present invention is related to bio-production of retinoids with improved purity profile, particularly bio-retinoids with a high percentage of retinyl acetate, wherein the flux towards retinol is increased and wherein the percentage of unwanted by-products such as dihydroretinoids or retinal is minimized to a range of below 10% by expressing retinol dehydrogenase (EC 1.1.1.105, RDH22) derived from Yarrowia lipolytica (Candida lipolytica) or Wickerhamomyces anomalus (Hanensula anomala).

公开(公告)号：US20240018564A1

公开(公告)日：2024-01-18

申请号：US18251167

申请日：2021-11-01

Applicant: DSM IP ASSETS B.V.

Inventor： Peter Louis HOUSTON ,  Valmik Kanubhai VYAS

IPC: C12P23/00 ,  C12N9/20 ,  C12N9/10

CPC classification number: C12P23/00 ,  C12N9/20 ,  C12Y301/01003 ,  C12Y203/01084 ,  C12N9/1029

Abstract: The present invention is related to a fermentative production of retinoids, including retinol or retinyl acetate, comprising cultivation of a retinoid-producing host cell, such as fungal host cell, particularly oleaginous host such as e.g. Yarrowia, in a two-phase culture system in the presence of vegetable oil as second phase. The retinoids present in the vegetable oil can be used without further isolation or purification steps as active ingredients in the food, feed, pharma or cosmetic industry.

公开(公告)号：US20220064607A1

公开(公告)日：2022-03-03

申请号：US17419079

申请日：2019-12-30

Applicant: DSM IP Assets B.V.

Inventor： Peter Louis HOUSTON ,  René Marcel DE JONG ,  Valmik Kanubhai VYAS ,  René VERWAAL ,  Jenna McMAHON ,  Anna SYMBOR-NAGRABSKA

IPC: C12N9/10 ,  C12P7/62 ,  C12N1/18

Abstract: The present invention is related to production of retinyl acetate generated via enzymatic conversion of retinol, said process including the use of modified enzymes with improved activity.

公开(公告)号：US20250109422A1

公开(公告)日：2025-04-03

申请号：US18834722

申请日：2023-02-01

Applicant: DSM IP ASSETS B.V.

Inventor： Peter Louis HOUSTON ,  Maria Elena MAYORGA ,  Valmik Kanubhai VYAS ,  Peter Scott YORGEY

IPC: C12P23/00 ,  C12N1/06 ,  C12N9/10 ,  C12N15/81

Abstract: The present invention is related to a novel process on biological production of lipophilic substances in an oleaginous host cell, particularly Yarrowia lipolytica, comprising modification in the N-glycosylation pathway, leading to higher yield and product purity with further facilitating the overall production process.

公开(公告)号：US20240216321A1

公开(公告)日：2024-07-04

申请号：US18527530

申请日：2023-12-04

Applicant: DSM IP Assets B.V.

Inventor： Adriana CARVALHO DE SOUZA ,  Peter Louis HOUSTON ,  Valmik Kanubhai VYAS ,  Angela WILDERMANN

IPC: A61K31/23 ,  A61K31/01 ,  A61K31/045 ,  A61K31/07 ,  A61K31/11 ,  A61K31/231

CPC classification number: A61K31/23 ,  A61K31/01 ,  A61K31/045 ,  A61K31/07 ,  A61K31/11 ,  A61K31/231

Abstract: Compositions of matter suitable for use in food, feed, pharmaceutical, and beauty care products comprising a retinoid component and a fermentative residue thereof are disclosed herein. In an embodiment, the retinoid component is present in a ratio by weight relative to the fermentative residue of 4:1 or greater, wherein the retinoid contains cis- and trans-isomers, wherein the cis-isomers are present by weight relative to the entire mixture of isomers in an amount less than 3 wt. %. Preferably, the compositions are bio-based. Also described are biotechnological methods of producing the compositions elsewhere described, along with further methods for processing such compositions into more stabilized, more commercially acceptable forms. Finally, also disclosed are food, feed, pharmaceutical, and beauty care products incorporating the compositions herein described.

公开(公告)号：US20230407344A1

公开(公告)日：2023-12-21

申请号：US18251189

申请日：2021-11-01

Applicant: DSM IP ASSETS B.V.

Inventor： Peter Louis HOUSTON ,  Valmik Kanubhai VYAS

IPC: C12P5/00

CPC classification number: C12P5/007

Abstract: The present invention is related to fermentative production of isoprenoids, comprising cultivation of a suitable host cell, such as fungal host cell, particularly oleaginous host cell, in an improved two-phase culture system.