摘要:
A method of cleaning or protecting surfaces by treatment with compositions comprising N-(3-oxododecanoyl)-L-homoserine lactone (OdDHL) blocking compounds and/or N-butyryl-L-homoserine lactone (BHL) analogs, either in combination or separately.
摘要:
The present invention relates to an immunogenic conjugate comprising a carrier molecule coupled to an autoinducer of a Gram negative bacteria The immunogenic conjugate, when combined with a pharmaceutically acceptable carrier, forms a suitable vaccine for mammals to prevent infection by the Gram negative bacteria The immunogenic conjugate is also used to raise and subsequently isolate antibodies or binding portions thereof which are capable of recognizing and binding to the autoinducer. The antibodies or binding portions thereof are utilized in a method of treating infections, a method of inhibiting autoinducer activity, and in diagnostic assays which detect the presence of autoinducers or autoinducer antagonists in fluid or tissue samples. TABLE OF CONTENTS Page 1. FIELD OF THE INVENTION1 2. BACKGROUND OF THE INVENTION1 3. SUMMARY OF THE INVENTION4 4. BRIEF DESCRIPTION OF THE DRAWINGS7 5. DETAILED DESCRIPTION OF THE INVENTION7 5.1 Isolation and Synthesis of Homoserine Lactones7 5.2 Bacterial Autoinducer Conjugation to a Carrier17 5.3 Generation of Antibodies to BAI Immunogenic conjugates19 5.4 Therapeutic Uses of Immunogenic conjugates22 or Antibodies Thereto 5.4.1 Administration and Formulation22 5.4.2 Effective Dose25 5.5 Diagnostic Methods27 6. EXAMPLE: SYNTHESIS32 Preparation of Compound C32 Preparation of Compound D32 Preparation of Compound 532 Preparation of Compound 633 Preparation of Compound 733 Preparation of Compound 833 Preparation of Compound 1034 Preparation of Compound 1134 Preparation of Compound 1335 Preparation of Compound 1235 Preparation of Compound 1435 Preparation of Compound 1536 Preparation of Compound 1736 Preparation of Compound 2137 Preparation of Compound 2237 Preparation of Compound 2438 Preparation of Compound 2538 Preparation of Compound 2738 Preparation of Compound 2939 7. EXAMPLE: PRODUCTION OF BAI ANTIBODIES39 7.1 PAI'S Role In Virulence39 7.2 Anti-PAI Polyclonal Antibodies40 7.3 Neutralization of PAI With Antibodies40
摘要:
Autoinducer molecules, e.g., N-(3-oxododecanoyl)homoserine lactone, for Pseudomonas aeruginosa are described. The molecules regulate gene expression in the bacterium. Therapeutic compositions and therapeutic methods involving analogs and/or inhibitors of the autoinducer molecules also are described. The molecules are useful for treating or preventing infection by Pseudomonas aeruginosa.
摘要:
Novel bacterial quinolone signal molecules and, more particularly, pseudomonas quinolone signal (“PQS”) molecules, e.g., 2-heptyl-3-hydroxy-4-quinolone, and analogs and derivatives thereof are described. Therapeutic compositions containing the molecules, and therapeutic methods, methods of for regulating gene expression, methods for identifying modulators of the autoinducer molecules, and methods of modulating quorum sensing signalling in bacteria using the compounds of the invention are also described.
摘要:
A method of detecting a specific microorganism comprising contacting said microorganism with bacteriocins from a microorganism of a genus which is taxonomically unrelated to said specific organism. The result of such contact may be utilized to detect the presence of a microorganism belonging to a taxonomically unrelated genus. Radio-labeled or fluorescein-labeled bacteriocins can be reacted with specific bacteria in a biological sample and the presence of such specific bacteria detected by removing excess bacteriocins and determining the presence of fluorescent or radioactive bacteria in the sample. Neisseria gonorrhoeae is identified by spotting bacteriocins on a plate of clinical material; or using a disk impregnated with bacteriocins placed on a plate inoculated with the clinical material; or the bacteriocins can be incorporated into one-half of a split agar plate, the identification being made on the basis of a zone of inhibition surrounding the spot where the bacteriocins were applied, or growth inhibition on the portion of the plate to which the bacteriocins were added.