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公开(公告)号：US09891218B2
公开(公告)日：2018-02-13
申请号：US15006153
申请日：2016-01-26
Applicant: EMD Millipore Corporation
Inventor： Chris Scott , Phillip Clark , Kurt E. Greenizen , Ryan A. Amara , Robert Colonna
IPC: G01N21/75 , G01N33/558 , G01N33/543 , B01L3/00 , B01L9/00 , G01N33/569 , B01J20/28
CPC classification number: G01N33/558 , B01J20/28033 , B01J20/28035 , B01J20/2804 , B01L3/5023 , B01L9/52 , B01L2200/025 , B01L2300/0609 , B01L2300/0681 , B01L2300/069 , B01L2300/0809 , B01L2300/12 , B01L2300/126 , B01L2400/0487 , B01L2400/049 , G01N33/54366 , G01N33/56911 , G01N2469/10 , G01N2469/20
Abstract: The embodiments disclosed herein are directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.
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公开(公告)号：US20160178625A1
公开(公告)日：2016-06-23
申请号：US15006153
申请日：2016-01-26
Applicant: EMD Millipore Corporation
Inventor： Chris Scott , Phillip Clark , Kurt E. Greenizen , Ryan A. Amara , Robert Colonna
IPC: G01N33/558 , B01L9/00 , B01L3/00
CPC classification number: G01N33/558 , B01J20/28033 , B01J20/28035 , B01J20/2804 , B01L3/5023 , B01L9/52 , B01L2200/025 , B01L2300/0609 , B01L2300/0681 , B01L2300/069 , B01L2300/0809 , B01L2300/12 , B01L2300/126 , B01L2400/0487 , B01L2400/049 , G01N33/54366 , G01N33/56911 , G01N2469/10 , G01N2469/20
Abstract: The embodiments disclosed herein are directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.
Abstract translation: 本文公开的实施方案涉及用于在印迹膜上进行化合物检测的装置。 该装置包括多个层，包括印迹膜下面的多孔支撑层，印迹膜上方的流量分布器，以及任选的流量分配器上的孔，以将液体包含到期望的区域并允许 降低这种液体的起始体积。 优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜，并且支撑层是格栅或烧结的多孔材料。 分配器和支撑件保持在一起以在膜周围形成一个信封。 在这样做时优选使用铰链，夹子和其它这样的装置。
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公开(公告)号：US20200330981A1
公开(公告)日：2020-10-22
申请号：US16607743
申请日：2018-05-10
Applicant: EMD Millipore Corporation
Inventor： Phillip Clark , Kurt E. Greenizen , John Doyle
Abstract: Filtration apparatus for the assay of biological and biochemical reactants, for example, is provided and includes a substrate such as a plate having one or more wells open at each end, and a porous membrane positioned in each well forming a discrete filtering area. The filtration apparatus includes a seal that is in a compressible relationship with the face of the porous membrane, the surface of the compression element, and the well wall. Each well includes a compression element, such as an internal well insert or sleeve, which compresses the seal so that the seal contacts the membrane face, the surface of the compression element, and the well wall in a liquid-tight manner. The compression element may be configured so that it is fixed in the well such as by an interference fit with the well wall or by bonding to a surface of the substrate.
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公开(公告)号：US20180372600A1
公开(公告)日：2018-12-27
申请号：US16115762
申请日：2018-08-29
Applicant: EMD Millipore Corporation
Inventor： Phillip Clark , Kurt Greenizen , John Doyle
Abstract: Fluid containment pressure vessel that eliminates the outer support housing present in certain conventional vessels, and provides an improved cap-to-body interface. In certain embodiments, the cap is threaded and configured to threadingly engage with the body of the pressure vessel, and has sufficient structural integrity to withstand the pressures in the device without the need for a support housing. In certain embodiments, an interlock mechanism is provided that prevents the cap from being opened (e.g., removed from the body) while the device is under pressure. In certain embodiments, a pressure relief valve is provided with a pre-loaded biasing mechanism to achieve the required pressure release rate. The vessel can be used for sample preparation, including purification or concentration of samples, particularly protein samples.
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公开(公告)号：US11623215B2
公开(公告)日：2023-04-11
申请号：US16607743
申请日：2018-05-10
Applicant: EMD Millipore Corporation
Inventor： Phillip Clark , Kurt E. Greenizen , John Doyle
Abstract: Filtration apparatus for the assay of biological and biochemical reactants, for example, is provided and includes a substrate such as a plate having one or more wells open at each end, and a porous membrane positioned in each well forming a discrete filtering area. The filtration apparatus includes a seal that is in a compressible relationship with the face of the porous membrane, the surface of the compression element, and the well wall. Each well includes a compression element, such as an internal well insert or sleeve, which compresses the seal so that the seal contacts the membrane face, the surface of the compression element, and the well wall in a liquid-tight manner. The compression element may be configured so that it is fixed in the well such as by an interference fit with the well wall or by bonding to a surface of the substrate.
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Patent Agency Ranking
公开(公告)号：US10088398B2
公开(公告)日：2018-10-02
申请号：US14994402
申请日：2016-01-13
Applicant: EMD Millipore Corporation
Inventor： Phillip Clark , Kurt Greenizen , John Doyle
Abstract: Fluid containment pressure vessel that eliminates the outer support housing present in certain conventional vessels, and provides an improved cap-to-body interface. In certain embodiments, the cap is threaded and configured to threadingly engage with the body of the pressure vessel, and has sufficient structural integrity to withstand the pressures in the device without the need for a support housing. In certain embodiments, an interlock mechanism is provided that prevents the cap from being opened (e.g., removed from the body) while the device is under pressure. In certain embodiments, a pressure relief valve is provided with a pre-loaded biasing mechanism to achieve the required pressure release rate. The vessel can be used for sample preparation, including purification or concentration of samples, particularly protein samples.
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公开(公告)号：US09897520B2
公开(公告)日：2018-02-20
申请号：US14974334
申请日：2015-12-18
Applicant: EMD Millipore Corporation
Inventor： Christopher A. Scott , Timothy K. Nadler , Kurt Greenizen , Louis Bonhomme , Sara D. Gutierrez , Masaharu Mabuchi , David Briggs , Phillip Clark , Ralph T. Scaduto
CPC classification number: G01N1/34 , B01D61/18 , B01D63/06 , B01D63/16 , B01D2315/02 , B01L3/5021 , B01L3/5085 , B01L2300/0618 , B01L2300/0681 , C07K1/16 , C07K1/22 , G01N1/405 , G01N1/4077 , G01N2001/4088 , Y10T436/25375
Abstract: Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.
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公开(公告)号：US20160228870A1
公开(公告)日：2016-08-11
申请号：US14994402
申请日：2016-01-13
Applicant: EMD Millipore Corporation
Inventor： Phillip Clark , Kurt Greenizen , John Doyle
CPC classification number: G01N1/4005 , B01L3/502 , B01L2200/026 , B01L2200/0689 , B01L2200/085 , B01L2200/087 , B01L2300/042 , B01L2300/048 , B01L2300/049 , B01L2300/0681 , B01L2300/14 , B01L2400/0487 , B01L2400/0605 , B01L2400/0633 , B01L2400/065
Abstract: Fluid containment pressure vessel that eliminates the outer support housing present in certain conventional vessels, and provides an improved cap-to-body interface. In certain embodiments, the cap is threaded and configured to threadingly engage with the body of the pressure vessel, and has sufficient structural integrity to withstand the pressures in the device without the need for a support housing. In certain embodiments, an interlock mechanism is provided that prevents the cap from being opened (e.g., removed from the body) while the device is under pressure. In certain embodiments, a pressure relief valve is provided with a pre-loaded biasing mechanism to achieve the required pressure release rate. The vessel can be used for sample preparation, including purification or concentration of samples, particularly protein samples.
Abstract translation: 流体容纳压力容器，其消除了存在于某些常规容器中的外部支撑壳体，并提供了改进的盖对体界面。 在某些实施例中，盖被螺纹并构造成与压力容器的主体螺纹接合，并且具有足够的结构完整性以承受装置中的压力而不需要支撑壳体。 在某些实施例中，提供了一种互锁机构，其在设备处于压力下时防止盖被打开（例如，从主体移除）。 在某些实施例中，泄压阀设置有预加载偏置机构以实现所需的压力释放速率。 该容器可用于样品制备，包括样品的纯化或浓缩，特别是蛋白质样品。
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公开(公告)号：US20150108058A1
公开(公告)日：2015-04-23
申请号：US14572825
申请日：2014-12-17
Applicant: EMD Millipore Corporation
Inventor： Phillip Clark , John Doyle
CPC classification number: B01D65/003 , B01D29/01 , B01D35/301 , B01D61/08 , B01D61/18 , B01D61/22 , B01D61/32 , B01D63/087 , B01D2201/204 , B01D2201/34 , B01D2201/342 , B01D2313/04 , Y10T29/49863 , Y10T156/10
Abstract: A vacuum filter device comprising a filter body having two holders on opposite sides of a filter. Each holder contains a closed container in a fluid-tight, sealed relationship. The filter is retained by a compression sealing element and the sealing element is maintained in place by a compression element formed of a ring. The compression element is bonded to a portion of the body to hold it, the sealing element and filter in place under a compression seal. Optionally a port in the compression element is formed to align with the vent of the device. Further, an alignment feature on the compression element ensures the port is in alignment with the vent of the device. The device also includes a vacuum port communicating with the downstream side of the filter, and hence the filtrate container.
Abstract translation: 一种真空过滤器装置，包括在过滤器的相对侧上具有两个保持器的过滤器主体。 每个支架都包含一个封闭的容器，流体密封，密封的关系。 过滤器由压缩密封元件保持，并且密封元件通过由环形成的压缩元件保持在适当位置。 压缩元件结合到主体的一部分以将其保持，密封元件和过滤器在压缩密封下就位。 可选地，压缩元件中的端口形成为与装置的通气口对准。 此外，压缩元件上的对准特征确保端口与设备的通风口对准。 该装置还包括与过滤器的下游侧连通并且因此与滤液容器连通的真空口。
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公开(公告)号：US20140017151A1
公开(公告)日：2014-01-16
申请号：US14031458
申请日：2013-09-19
Applicant: EMD Millipore Corporation
Inventor： Christopher A. Scott , Timothy K. Nadler , Kurt Greenizen , Louis Bonhomme , Sara D. Gutierrez , Masaharu Mabuchi , David Briggs , Phillip Clark , Ralph T. Scaduto
IPC: G01N1/34
CPC classification number: G01N1/34 , B01D61/18 , B01D63/06 , B01D63/16 , B01D2315/02 , B01L3/5021 , B01L3/5085 , B01L2300/0618 , B01L2300/0681 , C07K1/16 , C07K1/22 , G01N1/405 , G01N1/4077 , G01N2001/4088 , Y10T436/25375
Abstract: Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.
Abstract translation: 允许完全结合，洗涤，洗脱，缓冲交换和浓缩过程的样品制备装置，在多个装置之间没有样品转移的情况下进行。 该装置包括储存器，用于保持色谱介质的柱，用于保持过滤装置的保持器区域和出口。 过滤装置插入到离心装置的保持器区域中，并且组件可以放置在可选的保持器中。 具有或不具有任选的保持器的组件可以放置在用于离心的常规离心管中。 整个结合，洗涤，洗脱，缓冲液交换和浓缩步骤可以在没有任何移液管转移（和相关样品损失）的情况下进行，样品制备装置也可用于结合和洗涤步骤，在这种情况下，过滤 设备不需要，并且用于缓冲区交换和集中步骤，在这种情况下不需要介质。
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