公开(公告)号：US12007316B2

公开(公告)日：2024-06-11

申请号：US16965111

申请日：2019-01-30

Applicant: Georgia Tech Research Corporation

Inventor： Aaron C. Enten ,  Todd Sulchek

IPC: G01N1/34 ,  B01D61/18 ,  B01D61/22 ,  B01D63/08 ,  C12M1/26 ,  C12M3/00 ,  G01N1/40

CPC classification number: G01N1/34 ,  B01D61/18 ,  B01D61/22 ,  B01D63/087 ,  C12M21/08 ,  C12M33/14 ,  G01N1/4005 ,  B01D2311/16 ,  B01D2315/08 ,  G01N2001/4088

Abstract: Embodiments of the present disclosure relate generally to dead-end filtration systems and, more particularly, to pulse-modulated periodic backflush systems and methods for clearing fouling layers in dead-end filtration systems. In some embodiments, a controller may control the flow of fluid in the system from cycling from a forward flow to a reverse flow. In some embodiments, the controller may cycle from forward to reverse flow based on a volumetric flow ratio. Embodiments of the present disclosure describe optimal volumetric flow ratios for optimizing the break of cake in a dead-end filtration system. Embodiments of the present disclosure describe optimal volumetric flow ratios for optimizing recovery percentages of targeted particles.

公开(公告)号：US20220228965A1

公开(公告)日：2022-07-21

申请号：US17650033

申请日：2022-02-04

Applicant: Georgia Tech Research Corporation

Inventor： Todd Sulchek ,  Alexander Alexeev ,  Bushra Tasadduq

IPC: G01N15/10 ,  B01L3/00 ,  G01N1/34 ,  G01N33/50

Abstract: A microchannel for processing cells by compression of the cells including an inlet, ridges and an outlet. Each ridge including a compressive surface and a cell adhesion entity. The outlet configured to remove at least one of a first portion of the cells and a second portion of the cells from the microchannel. Each ridge oriented at an angle of from 25 degrees to 70 degrees relative to a center axis of the microchannel. The cell adhesion entity configured such that the first portion of the cells has a first adhesion property relative to the cell adhesion entity to follow a first trajectory through the microchannel. The cell adhesion entity further configured such that the second portion of the cells has a second adhesion property relative to the cell adhesion entity to follow a second trajectory through the microchannel. The first trajectory is different from the second trajectory.

公开(公告)号：US20200330990A1

公开(公告)日：2020-10-22

申请号：US16918349

申请日：2020-07-01

Applicant: GEORGIA TECH RESEARCH CORPORATION

Inventor： Todd Sulchek ,  Alexander Alexeev ,  Gonghao Wang

IPC: B01L3/00 ,  G01N15/02 ,  G01N33/50 ,  G01N33/48

Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.

公开(公告)号：US20240248093A1

公开(公告)日：2024-07-25

申请号：US18289868

申请日：2022-06-03

Applicant: Georgia Tech Research Corporation ,  Emory University

Inventor： Todd Sulchek ,  Ignacio Sanz ,  Frances Eun-Hyung LEE ,  Katily Ramirez

IPC: G01N33/577 ,  C07K16/10

CPC classification number: G01N33/577 ,  C07K16/1018

Abstract: An exemplary embodiment of the present disclosure provides a composition comprising a cell capable of secreting one or more molecules, the cell non-covalently attached to a particle, wherein the particle comprises a first linker linking a collector unit, the collector unit capable of binding to the one or more molecules secreted by the cell, optionally, wherein the one or more molecules secreted by the cell are bound to the first unit. The cell is non-covalently bound to the particle through a targeting unit affixed to the particle via a second linker. Also disclosed are methods of isolating cells with one or more particles comprising the same or different collector units and targeting units.

公开(公告)号：US11925934B2

公开(公告)日：2024-03-12

申请号：US16918349

申请日：2020-07-01

Applicant: GEORGIA TECH RESEARCH CORPORATION

Inventor： Todd Sulchek ,  Alexander Alexeev ,  Gonghao Wang

IPC: B01L3/00 ,  G01N15/02 ,  G01N33/48 ,  G01N33/49 ,  G01N33/50

CPC classification number: B01L3/502761 ,  G01N15/0272 ,  G01N33/48 ,  G01N33/5005 ,  B01L3/502776 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/086 ,  G01N15/01 ,  G01N2015/0288 ,  G01N33/491

Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.

公开(公告)号：US11268892B2

公开(公告)日：2022-03-08

申请号：US16348520

申请日：2017-11-08

Applicant: Georgia Tech Research Corporation

Inventor： Todd Sulchek ,  Alexander Alexeev ,  Bushra Tasadduq

IPC: B01L3/00 ,  G01N15/10 ,  G01N1/34 ,  G01N33/50

Abstract: Embodiments of the present disclosure can include a method comprising: providing a plurality of cells to a microchannel, the microchannel coated in at least one cell adhesion entity and comprising a compressive surface and a first outlet, the compressive surface defining a compression gap, flowing the plurality of cells through the microchannel, wherein the flowing comprises: compressing the plurality of cells underneath the compressive surface; and exposing the plurality of cells to the at least one cell adhesion entity, wherein the exposing causes a first portion of the cells having a first adhesion property to temporarily bind to the cell adhesion entity; and collecting the first portion of cells at the first outlet; wherein the compression gap has a height of from 75% to 95% an average diameter of the plurality of cells.

公开(公告)号：US11668638B2

公开(公告)日：2023-06-06

申请号：US17650033

申请日：2022-02-04

Applicant: Georgia Tech Research Corporation

Inventor： Todd Sulchek ,  Alexander Alexeev ,  Bushra Tasadduq

IPC: B01L3/00 ,  G01N15/10 ,  G01N1/34 ,  G01N33/50

CPC classification number: G01N15/1056 ,  B01L3/502761 ,  B01L3/502776 ,  G01N1/34 ,  G01N33/5005 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0877 ,  B01L2300/16 ,  B01L2400/086 ,  G01N2015/1006 ,  G01N2015/1081

Abstract: A microchannel for processing cells by compression of the cells including an inlet, ridges and an outlet. Each ridge including a compressive surface and a cell adhesion entity. The outlet configured to remove at least one of a first portion of the cells and a second portion of the cells from the microchannel. Each ridge oriented at an angle of from 25 degrees to 70 degrees relative to a center axis of the microchannel. The cell adhesion entity configured such that the first portion of the cells has a first adhesion property relative to the cell adhesion entity to follow a first trajectory through the microchannel. The cell adhesion entity further configured such that the second portion of the cells has a second adhesion property relative to the cell adhesion entity to follow a second trajectory through the microchannel. The first trajectory is different from the second trajectory.

公开(公告)号：US20200219266A1

公开(公告)日：2020-07-09

申请号：US16734749

申请日：2020-01-06

Applicant: Georgia Tech Research Corporation

Inventor： Peng Qiu ,  Todd Sulchek

IPC: G06T7/20 ,  G06K9/00

Abstract: A system for tracking single-cell movement trajectories is disclosed. The system can record, to a plurality of frames, cells (events) within a microfluidic device. Also, the system can identify an event within each frame including whether the event is a single cell or multiple cells. When the event appears differently between frames (e.g., single cell in one frame and multiple cells in another frame), the system can either segment or merge the cell(s). Then, the system can determine a trajectory for the events based on a position of the event in the frames. Further, the system can determine cell properties based on the trajectory of the events.

公开(公告)号：US11288815B2

公开(公告)日：2022-03-29

申请号：US16734749

申请日：2020-01-06

Applicant: Georgia Tech Research Corporation

Inventor： Peng Qiu ,  Todd Sulchek

IPC: G06T7/20 ,  G06K9/00

Abstract: A system for tracking single-cell movement trajectories is disclosed. The system can record, to a plurality of frames, cells (events) within a microfluidic device. Also, the system can identify an event within each frame including whether the event is a single cell or multiple cells. When the event appears differently between frames (e.g., single cell in one frame and multiple cells in another frame), the system can either segment or merge the cell(s). Then, the system can determine a trajectory for the events based on a position of the event in the frames. Further, the system can determine cell properties based on the trajectory of the events.

公开(公告)号：US20220062904A1

公开(公告)日：2022-03-03

申请号：US17524168

申请日：2021-11-11

Applicant: Georgia Tech Research Corporation

Inventor： Todd Sulchek ,  Alexander Alexeev ,  Anna Liu

IPC: B01L3/00 ,  C12M3/06 ,  C12M1/42 ,  C12M1/00 ,  C12N15/87

Abstract: Embodiments of the present disclosure can include a method for convective intracellular delivery including providing cells and molecules to a microchannel having compressive surfaces, wherein the compressive surfaces define compression gaps having a height of from 20 and 80% of the average cell diameter, and a plurality of relaxation spaces disposed between the compressive surfaces, flowing the cell medium through the microchannel, wherein as the cell medium flows through the microchannel, the plurality of cells undergo a convective intracellular delivery process comprising: compressing the plurality of cells, wherein the compressing causes the plurality of cells to undergo a loss in intracellular volume Vloss, and passing the plurality of cells to a first relaxation space, wherein the plurality of cells undergo a gain in volume Vgain and absorb a portion of the plurality of molecules.