公开(公告)号：US20230175039A1

公开(公告)日：2023-06-08

申请号：US18062260

申请日：2022-12-06

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. NELSON ,  Jijumon CHELLISERRY

IPC: C12Q1/6811 ,  C07H21/00 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869

CPC classification number: C12Q1/6811 ,  C07H21/00 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869

Abstract: The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.

公开(公告)号：US20210403982A1

公开(公告)日：2021-12-30

申请号：US17328312

申请日：2021-05-24

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. NELSON ,  Lyle J. ARNOLD, Jr. ,  Lizhong DAI ,  Steven PHELPS ,  Jijumon CHELLISERRY

IPC: C12Q1/6806 ,  C12Q1/6848 ,  C12Q1/70 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6865 ,  C12Q1/6825

Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

公开(公告)号：US20160298176A1

公开(公告)日：2016-10-13

申请号：US15191231

申请日：2016-06-23

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. NELSON ,  Jijumon CHELLISERRY

IPC: C12Q1/68

CPC classification number: C12Q1/6811 ,  C07H21/00 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2525/161 ,  C12Q2525/186 ,  C12Q2525/301 ,  C12Q2537/1373

Abstract: The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.

公开(公告)号：US20230383335A1

公开(公告)日：2023-11-30

申请号：US18345861

申请日：2023-06-30

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. NELSON ,  Jijumon CHELLISERRY

IPC: C12Q1/6811 ,  C07H21/00 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869

CPC classification number: C12Q1/6811 ,  C07H21/00 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869

Abstract: The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.

公开(公告)号：US20220162679A1

公开(公告)日：2022-05-26

申请号：US17541117

申请日：2021-12-02

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. NELSON ,  Jijumon CHELLISERRY

IPC: C12Q1/6811 ,  C07H21/00 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869

Abstract: The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.

公开(公告)号：US20210404019A1

公开(公告)日：2021-12-30

申请号：US17475625

申请日：2021-09-15

Applicant: Gen-Probe Incorporated

Inventor： Jijumon CHELLISERRY ,  Kui GAO ,  Jeffrey M. LINNEN

IPC: C12Q1/6893 ,  C12N1/06 ,  G01N33/48 ,  C12Q1/6806

Abstract: The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.

公开(公告)号：US20190185911A1

公开(公告)日：2019-06-20

申请号：US16241778

申请日：2019-01-07

Applicant: GEN-PROBE INCORPORATED

Inventor： Norman C. NELSON ,  Lyle J. ARNOLD ,  Lizhong DAI ,  Steven PHELPS ,  Jijumon CHELLISERRY

IPC: C12Q1/6806 ,  C12Q1/6865 ,  C12Q1/6825 ,  C12Q1/6848 ,  C12P19/34 ,  C12Q1/70 ,  C12Q1/68

CPC classification number: C12Q1/6806 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6848 ,  C12Q1/6865 ,  C12Q1/70 ,  C12Q2537/101 ,  C12Q2545/10

Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

公开(公告)号：US20240360435A1

公开(公告)日：2024-10-31

申请号：US18771684

申请日：2024-07-12

Applicant: Gen-Probe Incorporated

Inventor： Kui GAO ,  Jijumon CHELLISERRY ,  Jeffrey LINNEN

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/70

CPC classification number: C12N15/1003 ,  C12Q1/6806 ,  C12Q1/70 ,  Y02A50/30

Abstract: Disclosed herein are lysis reagents for lysing red blood cells, thereby releasing an analyte, such as RNA from a host or pathogen, in a form suitable for analysis. The reagent includes at least a buffer, a detergent and one or both of a chloride containing salt and an anti-coagulant. The reagent serves to lyse blood cells, protect the released analyte from degradation in the lysate, and is compatible with subsequent steps for analysis of the analyte such as target capture, amplification, detection, or sequencing.

公开(公告)号：US20220221382A1

公开(公告)日：2022-07-14

申请号：US17652172

申请日：2022-02-23

Applicant: Gen-Probe Incorporated

Inventor： Jeffrey M. LINNEN ,  Jijumon CHELLISERRY

IPC: G01N1/38 ,  C12Q1/6806 ,  C12Q1/04 ,  C12N15/10 ,  C12N1/06 ,  C12Q1/6893 ,  C12Q1/70

Abstract: A method for detecting the presence of a pathogen in a whole blood sample aliquot, includes providing a sample aliquot from each of a number of whole blood samples, thereby providing a plurality of whole blood sample aliquots, separately contacting each of the whole blood sample aliquots of the plurality of whole blood sample aliquots with a lysis reagent, whereby at least a portion of the blood cells in each of the whole blood sample aliquots lyse, pooling the lysed whole blood sample aliquots to form a pooled lysate, and testing the pooled lysate for presence of a pathogen. Identification of the presence of the pathogen in the pooled lysate indicates the presence of the pathogen in at least one of the whole blood sample aliquots.

公开(公告)号：US20200308661A1

公开(公告)日：2020-10-01

申请号：US16869720

申请日：2020-05-08

Applicant: Gen-Probe Incorporated

Inventor： Jijumon CHELLISERRY ,  Kui GAO ,  Jeffrey M. LINNEN

IPC: C12Q1/6893 ,  C12N1/06 ,  G01N33/48 ,  C12Q1/6806

Abstract: The invention provides a lysis reagent for lysing red blood cells, thereby releasing a target, such as RNA from a parasitic organism, in a form suitable for analysis. The reagent includes at least ammonium chloride and an anionic detergent, and may include an anti-coagulant. The reagent serves to lyse red blood cells, protect the released target from degradation in the lysate, and is compatible with subsequent steps for analysis of the target such as target capture, amplification, detection, or sequencing.