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1.发明授权公开(公告)号：US12007316B2
公开(公告)日：2024-06-11
申请号：US16965111
申请日：2019-01-30
Applicant: Georgia Tech Research Corporation
Inventor： Aaron C. Enten , Todd Sulchek
CPC classification number: G01N1/34 , B01D61/18 , B01D61/22 , B01D63/087 , C12M21/08 , C12M33/14 , G01N1/4005 , B01D2311/16 , B01D2315/08 , G01N2001/4088
Abstract: Embodiments of the present disclosure relate generally to dead-end filtration systems and, more particularly, to pulse-modulated periodic backflush systems and methods for clearing fouling layers in dead-end filtration systems. In some embodiments, a controller may control the flow of fluid in the system from cycling from a forward flow to a reverse flow. In some embodiments, the controller may cycle from forward to reverse flow based on a volumetric flow ratio. Embodiments of the present disclosure describe optimal volumetric flow ratios for optimizing the break of cake in a dead-end filtration system. Embodiments of the present disclosure describe optimal volumetric flow ratios for optimizing recovery percentages of targeted particles.
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公开(公告)号：US20220228965A1
公开(公告)日：2022-07-21
申请号：US17650033
申请日：2022-02-04
Applicant: Georgia Tech Research Corporation
Inventor： Todd Sulchek , Alexander Alexeev , Bushra Tasadduq
Abstract: A microchannel for processing cells by compression of the cells including an inlet, ridges and an outlet. Each ridge including a compressive surface and a cell adhesion entity. The outlet configured to remove at least one of a first portion of the cells and a second portion of the cells from the microchannel. Each ridge oriented at an angle of from 25 degrees to 70 degrees relative to a center axis of the microchannel. The cell adhesion entity configured such that the first portion of the cells has a first adhesion property relative to the cell adhesion entity to follow a first trajectory through the microchannel. The cell adhesion entity further configured such that the second portion of the cells has a second adhesion property relative to the cell adhesion entity to follow a second trajectory through the microchannel. The first trajectory is different from the second trajectory.
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公开(公告)号：US20200330990A1
公开(公告)日：2020-10-22
申请号：US16918349
申请日：2020-07-01
Applicant: GEORGIA TECH RESEARCH CORPORATION
Inventor： Todd Sulchek , Alexander Alexeev , Gonghao Wang
Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.
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4.发明公开公开(公告)号：US20240248093A1
公开(公告)日：2024-07-25
申请号：US18289868
申请日：2022-06-03
Applicant: Georgia Tech Research Corporation , Emory University
Inventor： Todd Sulchek , Ignacio Sanz , Frances Eun-Hyung LEE , Katily Ramirez
IPC: G01N33/577 , C07K16/10
CPC classification number: G01N33/577 , C07K16/1018
Abstract: An exemplary embodiment of the present disclosure provides a composition comprising a cell capable of secreting one or more molecules, the cell non-covalently attached to a particle, wherein the particle comprises a first linker linking a collector unit, the collector unit capable of binding to the one or more molecules secreted by the cell, optionally, wherein the one or more molecules secreted by the cell are bound to the first unit. The cell is non-covalently bound to the particle through a targeting unit affixed to the particle via a second linker. Also disclosed are methods of isolating cells with one or more particles comprising the same or different collector units and targeting units.
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公开(公告)号：US11925934B2
公开(公告)日：2024-03-12
申请号：US16918349
申请日：2020-07-01
Applicant: GEORGIA TECH RESEARCH CORPORATION
Inventor： Todd Sulchek , Alexander Alexeev , Gonghao Wang
CPC classification number: B01L3/502761 , G01N15/0272 , G01N33/48 , G01N33/5005 , B01L3/502776 , B01L2200/0652 , B01L2300/0816 , B01L2300/0864 , B01L2400/086 , G01N15/01 , G01N2015/0288 , G01N33/491
Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.
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Patent Agency Ranking
公开(公告)号：US11268892B2
公开(公告)日：2022-03-08
申请号：US16348520
申请日：2017-11-08
Applicant: Georgia Tech Research Corporation
Inventor： Todd Sulchek , Alexander Alexeev , Bushra Tasadduq
Abstract: Embodiments of the present disclosure can include a method comprising: providing a plurality of cells to a microchannel, the microchannel coated in at least one cell adhesion entity and comprising a compressive surface and a first outlet, the compressive surface defining a compression gap, flowing the plurality of cells through the microchannel, wherein the flowing comprises: compressing the plurality of cells underneath the compressive surface; and exposing the plurality of cells to the at least one cell adhesion entity, wherein the exposing causes a first portion of the cells having a first adhesion property to temporarily bind to the cell adhesion entity; and collecting the first portion of cells at the first outlet; wherein the compression gap has a height of from 75% to 95% an average diameter of the plurality of cells.
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公开(公告)号：US20250145924A1
公开(公告)日：2025-05-08
申请号：US18832684
申请日：2023-01-25
Applicant: Georgia Tech Research Corporation
Inventor： Todd Sulchek , Eric Dixon , Peter Shankles
Abstract: A cell deactivation device includes a cell deactivation container. The cell deactivation container includes a plurality of microfluidic channels, a lid covering the plurality of microfluidic channels, the lid having a thickness of less than about 150 microns, and a distribution manifold configured to distribute a fluid to the plurality of microfluidic channels. The cell deactivation device further includes an irradiation source configured to generate an electron beam and transmit the electron beam through the lid of the cell deactivation container and into a fluid passing through the plurality of microfluidic channels.
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公开(公告)号：US11668638B2
公开(公告)日：2023-06-06
申请号：US17650033
申请日：2022-02-04
Applicant: Georgia Tech Research Corporation
Inventor： Todd Sulchek , Alexander Alexeev , Bushra Tasadduq
CPC classification number: G01N15/1056 , B01L3/502761 , B01L3/502776 , G01N1/34 , G01N33/5005 , B01L2200/0652 , B01L2200/0668 , B01L2300/0877 , B01L2300/16 , B01L2400/086 , G01N2015/1006 , G01N2015/1081
Abstract: A microchannel for processing cells by compression of the cells including an inlet, ridges and an outlet. Each ridge including a compressive surface and a cell adhesion entity. The outlet configured to remove at least one of a first portion of the cells and a second portion of the cells from the microchannel. Each ridge oriented at an angle of from 25 degrees to 70 degrees relative to a center axis of the microchannel. The cell adhesion entity configured such that the first portion of the cells has a first adhesion property relative to the cell adhesion entity to follow a first trajectory through the microchannel. The cell adhesion entity further configured such that the second portion of the cells has a second adhesion property relative to the cell adhesion entity to follow a second trajectory through the microchannel. The first trajectory is different from the second trajectory.
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公开(公告)号：US20200219266A1
公开(公告)日：2020-07-09
申请号：US16734749
申请日：2020-01-06
Applicant: Georgia Tech Research Corporation
Inventor： Peng Qiu , Todd Sulchek
Abstract: A system for tracking single-cell movement trajectories is disclosed. The system can record, to a plurality of frames, cells (events) within a microfluidic device. Also, the system can identify an event within each frame including whether the event is a single cell or multiple cells. When the event appears differently between frames (e.g., single cell in one frame and multiple cells in another frame), the system can either segment or merge the cell(s). Then, the system can determine a trajectory for the events based on a position of the event in the frames. Further, the system can determine cell properties based on the trajectory of the events.
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公开(公告)号：US11288815B2
公开(公告)日：2022-03-29
申请号：US16734749
申请日：2020-01-06
Applicant: Georgia Tech Research Corporation
Inventor： Peng Qiu , Todd Sulchek
Abstract: A system for tracking single-cell movement trajectories is disclosed. The system can record, to a plurality of frames, cells (events) within a microfluidic device. Also, the system can identify an event within each frame including whether the event is a single cell or multiple cells. When the event appears differently between frames (e.g., single cell in one frame and multiple cells in another frame), the system can either segment or merge the cell(s). Then, the system can determine a trajectory for the events based on a position of the event in the frames. Further, the system can determine cell properties based on the trajectory of the events.
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