摘要:
In order to lease threads (F), in particular warp threads into guide elements (2) of a handling device (1), such as combs or reeds, the threads (F) are first leased in sequence into a leasing comb (11) and are fixed thereto. The threads (F) are subsequently guided through the device (1) by means of the leasing comb (11). During this process, the threads (F) are taken up by the guide elements (2).
摘要:
The invention relates to a dryer (1) for drying continuously conveyed products, e.g. a warp, comprising essentially a first dryer array (2) and a second dryer array (3). A passage (4) for passing through the product (W) is formed between the dryer arrays (2, 3). The second dryer array (3) is configured in such a way as to be able to move in relation to the first dryer array (2). When the dryer (1) is in stop position (S), the second dryer array (3) is disposed relative to the first dryer array (2) in such a way that the cross section of the passage (4) is bigger than in the operating position (B).
摘要:
The present invention relates to a method of preparing an insoluble separation matrix, which method comprises salt-treatment of a polysaccharide gel followed by cross-linking of the polysaccharide polymers. In one embodiment, the method comprises providing an aqueous solution of a gelatable native polysaccharide; lowering the temperature of the polysaccharide solution to a value below its gelling point; salt-treatment by adding at least one salt to the resulting polysaccharide; and cross-linking the salt-treated polysaccharide. The polysaccharide may be prepared e.g. into particles, membranes or monoliths.
摘要:
The present invention relates to a method of preparing a porous cross-linked polysaccharide chromatography matrix, which comprises to provide an aqueous solution of a gellable polysaccharide, wherein part of the hydroxyl groups are substituted with groups which are not susceptible to nucleophilic attack; to provide essentially spherical droplets of the substituted polysaccharide solution; to form a gel of the substituted polysaccharide solution; and to cross-link the gel by adding a cross-linking agent. The invention also encompasses a chromatography column packed with a matrix so produced as well as use thereof in e.g. protein purification.
摘要:
The present invention relates to a microcarrier onto the surface of which a cationic compound has been immobilised via a guanidine group. The microcarrier is capable of attachment of cells, e.g. via charge-based interaction, and is advantageously used as a support in the culture of cells. Said compound may comprise one or two amino acids, such as arginine (Arg) or a dipeptide. The invention also relates to a method of preparing a polycationic microcarrier, which method comprises to immobilise a compound that comprises at least one guanidine group to an epoxide-activated substrate.
摘要:
The present invention relates to a method of preparing a porous cross-linked polysaccharide chromatography matrix, which comprises to provide an aqueous solution of a gellable polysaccharide, wherein part of the hydroxyl groups are substituted with groups which are not susceptible to nucleophilic attack; to provide essentially spherical droplets of the substituted polysaccharide solution; to form a gel of the substituted polysaccharide solution; and to cross-link the gel by adding a cross-linking agent. The invention also encompasses a chromatography column packed with a matrix so produced as well as use thereof in e.g. protein purification.
摘要:
A method for the manufacture of a solid porous separation material based on a polysaccharide, said method comprising the steps of: (a) providing an aqueous solution (I) of a polysaccharide, (b) solidifying the solution, preferably by transforming the solution to a gel, and (c) optionally cross-linking the polysaccharide, with the proviso that, if step (c) is present, steps (b) and (c) may be carried out simultaneously. The method is characterised in that the polysaccharide provided in step (a) is modified by being inter-molecularly cross-linked to an extent such that the viscosity of solution (I) is at least 110%, preferably at least 200%, of the viscosity of an aqueous solution (II) of the corresponding polysaccharide which has not been inter-molecularly cross-linked and which is present in the same concentration as the inter-molecularly cross-linked polysaccharide is in solution (I).
摘要:
A process for the production of a porous cross-linked polysaccharide gel and a gel obtainable by the following steps: a) preparing a solution or dispersion of the polysaccharide; b) adding a bifunctional cross-linking agent having one active site and one inactive site to the solution or dispersion from step a); c) reacting hydroxylgroups of the polysaccharide with the active site of the cross-linking agent; d) forming a polysaccharide gel; e) activating the inactive site of the cross-linking agent; f) reacting the activated site from step e) with hydroxylgroups of the polysaccharide gel, whereby cross-linking of the gel takes place. The cross-linked polysaccharide gel obtained can further be cross-linked by conventional methods, one or several times.
摘要:
The present invention relates to a separation matrix comprised of porous particles to which antibody-binding protein ligands have been immobilized, wherein the ligand density is in the range of 5.0-10 mg/ml; the gel phase distribution coefficient of the particles expressed as Kav for a dextran of size 110 kDa is above 0.65 and the median particle diameter is between 65-84 μm. The carbohydrate material is preferably highly cross-linked agarose.
摘要:
The present invention is a process of manufacture of one or more polysaccharide beads, comprising generating an aerosol of an aqueous polysaccharide solution, cooling the droplets of said aerosol in air to initiate gelling thereof and collecting droplets as gelled beads in a liquid or on a surface, characterised by adding a hydrophilic vapour pressure-lowering agent to said polysaccharide solution.