-
公开(公告)号：US5221608A
公开(公告)日：1993-06-22
申请号：US428494
申请日：1989-10-26
IPC分类号： B01L7/00 , C07D311/18 , C07D493/04 , C07D519/00 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： C07D493/04 , B01L7/00 , B01L7/52 , C07D311/18 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/6848 , C12Q1/6876 , C12Q1/703 , Y10S435/808 , Y10S436/805
摘要： Method for treating nucleic acid that is to be amplified such that the nucleic acid is, after amplification, subsequently unamplifiable. Nucleic acid amplification is performed in a reaction containing means in the presence of an isopsoralen. The reaction is thereafter irradiated to render the amplified nucleic acid substantially unamplifiable.
摘要翻译： 用于处理待扩增的核酸使得核酸在扩增后随后不可扩增的方法。 在含有补骨脂素的装置中进行核酸扩增。 然后将反应物照射以使扩增的核酸基本上不可扩增。
-
公开(公告)号：US5532145A
公开(公告)日：1996-07-02
申请号：US979789
申请日：1992-11-20
IPC分类号： B01L7/00 , C07D311/18 , C07D493/04 , C07D519/00 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/70 , C07H21/04
CPC分类号： C12Q1/6848 , B01L7/00 , B01L7/52 , C07D311/18 , C07D493/04 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/6876 , C12Q1/703 , Y10S435/81
摘要： Method useful for solving the problem of contamination of protein with nucleic acid. Rendering amplifiable contaminating nucleic acid in enzyme preparations substantially unamplifiable prior to use of the enzyme.
摘要翻译： 用于解决核酸污染蛋白质问题的方法。 在使用酶之前，在酶制剂中呈现可扩增的污染性核酸基本上是不可扩增的。
-
公开(公告)号：US5532146A
公开(公告)日：1996-07-02
申请号：US263020
申请日：1994-06-20
IPC分类号： B01L7/00 , C07D311/18 , C07D493/04 , C07D519/00 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： C07D493/04 , B01L7/00 , B01L7/52 , C07D311/18 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/6848 , Y10S435/81
摘要： Methods for sterilization of ligase-based amplification products, including products of the Ligation Amplification Reaction (LAR), involving treatment of nucleic acid after amplification to render such nucleic acid incapable of serving as a template for further amplification. Both random and site-specific addition of sterilizing compounds, and more specifically, photoactivation compounds, render amplification product subsequently unamplifiable. Self-sterilizing primers are also employed allowing for a homogenous detection of ligase-based amplification product.
摘要翻译： 连接酶扩增产物的灭菌方法，包括连接扩增反应（LAR）的产物，涉及在扩增后处理核酸，使得这种核酸不能作为进一步扩增的模板。 杀菌化合物，特别是光活化化合物的随机和位点特异性添加使随后的扩增产物不可扩增。 还使用自消毒引物，允许基于连接酶的扩增产物的均匀检测。
-
公开(公告)号：US5854967A
公开(公告)日：1998-12-29
申请号：US805456
申请日：1997-02-25
IPC分类号： B01J19/12 , B01L7/00 , C07D493/04 , C07D519/00 , C12Q1/68 , G11C11/412 , H01L27/11
CPC分类号： B01L7/52 , B01J19/123 , B01L7/00 , C07D493/04 , C12Q1/6848 , G11C11/412 , H01L27/11 , Y10S204/902
摘要： A device comprising a light source, a sample holder and a temperature control chamber. The sample holder supports the vessels in a fixed relationship relative to the light source. The temperature control chamber surrounds the sample holder and vessels, so that a temperature control fluid may be passed around the sample vessels effectively maintaining the temperature of the sample vessels within a desired temperature range.
-
公开(公告)号：US5683661A
公开(公告)日：1997-11-04
申请号：US477670
申请日：1995-06-07
IPC分类号： B01J19/12 , B01L7/00 , C07D493/04 , C07D519/00 , C12Q1/68 , G11C11/412 , H01L27/11 , G01N21/85
CPC分类号： B01L7/52 , B01J19/123 , B01L7/00 , C07D493/04 , C12Q1/6848 , G11C11/412 , H01L27/11 , Y10S204/902
摘要： A device comprising a light source, a sample holder and a temperature control chamber. The sample holder supports the vessels in a fixed relationship relative to the light source. The temperature control chamber surrounds the sample holder and vessels, so that a temperature control fluid may be passed around the sample vessels effectively maintaining the temperature of the sample vessels within a desired temperature range.
摘要翻译： 一种包括光源，样品架和温度控制室的装置。 样品支架相对于光源以固定的关系支撑血管。 温度控制室围绕样品保持器和容器，使得温度控制流体可以有效地将样品容器的温度保持在期望的温度范围内。
-
公开(公告)号：US5184020A
公开(公告)日：1993-02-02
申请号：US428510
申请日：1989-10-26
IPC分类号： B01J19/12
CPC分类号： B01J19/123
摘要： A device comprising a light source, a sample holder and a temperature control chamber. The sample holder supports the vessels in a fixed relationship relative to the light source. The temperature control chamber surrounds the sample holder and vessels, so that a temperature control fluid may be passed around the sample vessels effectively maintaining the temperature of the sample vessels within a desired temperature range.
摘要翻译： 一种包括光源，样品架和温度控制室的装置。 样品支架相对于光源以固定的关系支撑血管。 温度控制室围绕样品保持器和容器，使得温度控制流体可以有效地将样品容器的温度保持在期望的温度范围内。
-
公开(公告)号：US5503721A
公开(公告)日：1996-04-02
申请号：US320126
申请日：1994-10-07
IPC分类号： B01J19/12 , B01L7/00 , C07D493/04 , C07D519/00 , C12Q1/68 , G11C11/412 , H01L27/11
CPC分类号： B01L7/52 , B01J19/123 , B01L7/00 , C07D493/04 , C12Q1/6848 , G11C11/412 , H01L27/11 , Y10S204/902
摘要： A method for activating photoreactive compounds by supporting sample vessels containing a photoreactive compound at a selected temperature and irradiating the sample vessels while holding the sample vessels at a fixed relationship with the irradiating source so as to uniformly irradiate the compound.
摘要翻译： 一种通过在选定温度下支持含有光反应性化合物的样品容器来激活光反应性化合物的方法，并且在将样品容器与照射源保持固定关系的同时照射样品容器，以均匀地照射该化合物。
-
8.发明授权Methods for inactivating bacteria in blood preparations with 8-methoxypsoralen 失效用8-甲氧基补骨脂素灭活血液制剂中细菌的方法
公开(公告)号：US5288605A
公开(公告)日：1994-02-22
申请号：US844790
申请日：1992-03-02
IPC分类号： A61J3/00 , A01N1/02 , A61K31/37 , A61K35/14 , A61K41/00 , A61L2/00 , A61M1/36 , A61M37/00 , A61N1/30
CPC分类号： A61K31/37 , A01N1/0215 , A01N1/0242 , A01N1/0294 , A61K41/0019 , A61L2/0011 , A61M1/3616 , A61M1/3683 , A61M1/3686
摘要： Methods and compositions are described for treating contaminants in material intended for in vivo use, and in particular blood and blood products for human use. Contaminants in blood cell preparations are inactivated using 8-methoxypsoralen prior to long term storage and transfusion.
摘要翻译： 描述了用于治疗旨在体内使用的材料中的污染物的方法和组合物，特别是用于人类的血液和血液制品。 血液细胞制剂中的污染物在长期储存和输血之前使用8-甲氧基补骨脂素灭活。
-
公开(公告)号：US5139940A
公开(公告)日：1992-08-18
申请号：US427303
申请日：1989-10-26
IPC分类号： B01L7/00 , C07D311/18 , C07D493/04 , C07D519/00 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： C07D493/04 , B01L7/00 , B01L7/52 , C07D311/18 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/6848 , Y10S435/81
摘要： The invention is directed to isopsoralen compound compositions that contain a variety of substitutions which yet permit their binding to nucleic acid polymers. Reaction conditions that activate these bound isopsoralens results in covalent crosslinking to the nucleic acid polymers. The resultant complex is inhibited from being a template for the template-directed enzymatic synthesis of a complementary nucleic acid polymer. Beside the isopsoralens compositions, the invention includes their synthesis, the nucleic acid complex production and detection, and the above inhibitory method of use of the isopsoralens.
摘要翻译： 本发明涉及含有多种替代物的异补骨脂素化合物组合物，其可以与核酸聚合物结合。 激活这些结合的异补骨脂素的反应条件导致与核酸聚合物的共价交联。 所得复合物被抑制为用于互补核酸聚合物的模板定向酶合成的模板。 除了异消消旋体组合物之外，本发明包括它们的合成，核酸复合体的产生和检测以及上述异丙屈肌酸酯的抑制方法。
-
公开(公告)号：US5284940A
公开(公告)日：1994-02-08
申请号：US614921
申请日：1990-11-14
申请人： Lily Lin , Stephen T. Isaacs , John E. Hearst
发明人： Lily Lin , Stephen T. Isaacs , John E. Hearst
CPC分类号： C12Q1/703 , C07K16/44 , C12N15/1003 , C12Q1/6806 , C12Q1/6848
摘要： Methods and compounds for the preparation of nucleic acid samples. Polymerase inhibitors are treated so as to allow for amplification of nucleic acid from biological sources, including whole blood.
摘要翻译： 用于制备核酸样品的方法和化合物。 处理聚合酶抑制剂以允许从生物来源（包括全血）扩增核酸。
-
-
-
-
-
-
-
-
-
搜索结果
38 条记录 (耗时 0.046 秒)
