Method and apparatus for testing cardiotoxicity and evaluating cardiomyocytes
    8.
    发明授权
    Method and apparatus for testing cardiotoxicity and evaluating cardiomyocytes 有权
    用于测试心脏毒性和评估心肌细胞的方法和装置

    公开(公告)号:US09447447B2

    公开(公告)日:2016-09-20

    申请号:US13876992

    申请日:2011-09-30

    摘要: In the present invention, a cardiomyocyte cluster is disposed on a transparent substrate, and the quality of the cardiomyocytes is evaluated from the response of the cells to a forced pulsation stimulus applied to the cardiomyocytes. The cardiomyocyte cluster is disposed on the transparent substrate, and is exposed to the flow of a liquid containing an agent in a manner so that the agent acts on the cells, which configure a network. The extent of cardiac toxicity resulting from the agent is evaluated from measuring the fluctuations obtained from a comparison of adjacent cardiomyocytes of the network.

    摘要翻译: 在本发明中,将心肌细胞簇设置在透明基质上,根据细胞对施加于心肌细胞的强制脉动刺激的反应来评价心肌细胞的质量。 将心肌细胞簇设置在透明基板上,并且以使得试剂作用于构成网络的细胞的方式暴露于含有试剂的液体的流动。 通过测量从网络的相邻心肌细胞的比较获得的波动来评估由该试剂产生的心脏毒性的程度。

    Cell measuring and sorting chip
    9.
    发明授权
    Cell measuring and sorting chip 有权
    电池测量和分选芯片

    公开(公告)号:US08361412B2

    公开(公告)日:2013-01-29

    申请号:US11317004

    申请日:2005-12-27

    IPC分类号: B01L3/00

    摘要: A cell sorting device using an inexpensive chip capable of being exchanged for each sample. The chip includes: a first flow path allowing buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow buffer fluid not containing cells to flow down; a fourth flow path which allows the buffer fluid as a single flow path formed by joining the buffer fluids in the other three flow paths; a cell detecting region for detecting cells flowing with the buffer fluid down the fourth flow path; and a cell sorting region for sorting the cells according to a type of the cells detected. The first to fourth flow paths are cascaded, are supplied with the buffer fluid from reservoirs with the same fluid level, and have substantially the same width or cross-section area.

    摘要翻译: 一种使用廉价芯片的细胞分选装置,能够对每个样品进行交换。 芯片包括:允许包含电池的缓冲流体向下流动的第一流动路径; 第二和第三流动路径,其中第一流动路径在其间并且允许不包含电池的缓冲流体向下流动; 第四流路,其允许缓冲流体作为通过连接其它三个流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域,用于检测沿着第四流动路径流动的缓冲液的细胞; 以及用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一至第四流动路径级联,从具有相同流体水平的储存器提供缓冲流体,并且具有基本上相同的宽度或横截面面积。

    Micro-chamber processing apparatus for cell culture and method
    10.
    发明授权
    Micro-chamber processing apparatus for cell culture and method 有权
    用于细胞培养和方法的微室处理装置

    公开(公告)号:US08247222B2

    公开(公告)日:2012-08-21

    申请号:US13036600

    申请日:2011-02-28

    IPC分类号: C12M1/00 C12M1/34

    摘要: This invention relates to a method and apparatus for processing cell cultures. The apparatus comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source, the absorption layer having absorbency in visible and infrared regions, and the gel-like material being a substance which has a gel dissolution temperature of 100 degree C. or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions, and the light source having a monochromatic light in the specific wave length, wherein the light source is disposed such that it irradiates on the absorption layer and/or the gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on the transparent base plate.

    摘要翻译: 本发明涉及一种用于处理细胞培养物的方法和装置。 该装置包括一个微室,该微室包括不超过一个吸收层和至少一个凝胶层,依次层叠在透明基板上,在可见光和红外区域中没有明显的吸收性,以及至少一个光源,吸收 在可见光和红外区域具有吸收性的层,凝胶状材料是凝胶溶解温度为100℃以下的物质,加热时的溶胶化物,在室温下处于凝胶状态,具有特定的吸光度 可见光和红外区域的波长,以及具有特定波长的单色光的光源,其中光源被设置成使其照射在吸收层和/或凝胶层上,除了当没有吸收 在透明基板上层叠由凝胶状材料构成的至少两层。