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公开(公告)号：US09808799B2
公开(公告)日：2017-11-07
申请号：US14462640
申请日：2014-08-19
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Joseph Amshey , Daniel Bezdek , Espir Kahatt , Alexander Khorlin , Lance Parsons , Todd Peterson , Timothy Powers , Michael Thacker , Timothy Updyke , Kornelija Zgonc
CPC classification number: B01L3/502715 , B01L3/50273 , B01L7/00 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/10 , B01L2200/16 , B01L2300/0636 , B01L2300/0681 , B01L2300/0867 , B01L2300/088 , B01L2300/123 , B01L2400/0481 , B01L2400/0487 , B01L2400/0605 , B01L2400/0655 , G01N2035/00306 , G01N2035/1037 , Y10T436/2575
Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.
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Patent Agency Ranking
公开(公告)号：US11235323B2
公开(公告)日：2022-02-01
申请号：US16557192
申请日：2019-08-30
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Joseph Amshey , Daniel Bezdek , Espir Kahatt , Alexander Khorlin , Lance Parsons , Todd Peterson , Timothy Powers , Michael Thacker , Timothy Updyke , Kornelija Zgonc
Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.
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3.发明授权公开(公告)号：US10557174B2
公开(公告)日：2020-02-11
申请号：US15080801
申请日：2016-03-25
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Gordon A. Janaway , Mark Andersen , Kornelija Zgonc , Michael C. Pallas , Marcin Sikora , Casey R. McFarland , Ferrier N. Le , Haopeng Wang , Jian Gong , Gothami Padmabandu
IPC: C12Q1/6886 , C12Q1/686 , G01N21/64
Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.
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公开(公告)号：US10434509B2
公开(公告)日：2019-10-08
申请号：US15719130
申请日：2017-09-28
Applicant: Life Technologies Corporation
Inventor： Joseph Amshey , Daniel Bezdek , Espir Kahatt , Alexander Khorlin , Lance Parsons , Todd Peterson , Timothy Powers , Michael Thacker , Timothy Updyke , Kornelija Zgonc
Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.
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5.发明申请SYSTEM AND METHOD FOR DETERMINING COPIES-PER-UNIT-VOLUME USING PCR AND FLOW CONTROL OF DROPLETS 审中-公开使用PCR和流量控制法确定复制单位体积的系统和方法公开(公告)号：US20160208342A1
公开(公告)日：2016-07-21
申请号：US15080801
申请日：2016-03-25
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Gordon A. Janaway , Mark Andersen , Kornelija Zgonc , Michael C. Pallas , Marcin Sikora , Casey R. McFarland , Ferrier N. Le , Haopeng Wang , Jian Gong , Gothami Padmabandu
CPC classification number: C12Q1/6886 , C12Q1/686 , C12Q2600/158 , C12Q2600/178 , G01N21/6486 , C12Q2537/16 , C12Q2563/143 , C12Q2563/159 , C12Q2565/626
Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.
Abstract translation: 提供了用于定量样品中靶核酸的方法和系统。 该方法包括形成多个离散的样本部分。 多个离散样品部分中的每一个包含样品的一部分和反应混合物。 该方法还包括放大多个离散样本部分以形成多个离散处理的样本部分。 至少一个离散加工的样品部分含有核酸扩增反应产物。 从所述多个离散处理样品部分中的至少一个检测荧光信号以确定所述至少一种靶核酸的存在。 该方法还包括确定多个多个离散处理的样本部分的相应体积，以及估计样品中至少一个靶核酸的每单位体积的拷贝数。 估计每单位体积的拷贝数是基于确定为在其中含有至少一种靶核酸的离散加工样品部分的数量。
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公开(公告)号：US20150044758A1
公开(公告)日：2015-02-12
申请号：US14462640
申请日：2014-08-19
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Joseph Amshey , Daniel Bezdek , Espir Kahatt , Alexander Khorlin , Lance Parsons , Todd Peterson , Timothy Powers , Michael Thacker , Timothy Updyke , Kornelija Zgonc
IPC: B01L3/00
CPC classification number: B01L3/502715 , B01L3/50273 , B01L7/00 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/10 , B01L2200/16 , B01L2300/0636 , B01L2300/0681 , B01L2300/0867 , B01L2300/088 , B01L2300/123 , B01L2400/0481 , B01L2400/0487 , B01L2400/0605 , B01L2400/0655 , G01N2035/00306 , G01N2035/1037 , Y10T436/2575
Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.
Abstract translation: 本发明提供了用于自动化生物处理的系统，装置，装置和方法。 适用于本发明的方案和生物处理方法的实例包括但不限于免疫沉淀，染色质免疫沉淀，重组蛋白分离，核酸分离和分离，蛋白质标记，分离和分离，细胞分离和分离，食品安全分析和自动珠 基于分离。 在一些实施方案中，本发明提供自动化系统，自动化装置，自动化盒和western印迹处理的自动化方法。 其他实施方案包括自动化系统，自动化装置，自动化盒和用于分离，制备和纯化核酸例如DNA或RNA或其片段的自动化方法，包括质粒DNA，基因组DNA，细菌DNA，病毒DNA和任何其它DNA， 以及用于自动化系统，自动化设备，自动化盒和用于处理，分离和纯化蛋白质，肽等的自动化盒和自动化方法。
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