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1.发明授权Process for the microbial production of amino acids by boosted activity of export carriers 失效通过出口承运人的加强活动，微生物生产氨基酸的过程公开(公告)号：US06858406B1
公开(公告)日：2005-02-22
申请号：US09105117
申请日：1998-06-17
申请人： Marina Vrlijc , Lothar Eggeling , Hermann Sahm
发明人： Marina Vrlijc , Lothar Eggeling , Hermann Sahm
IPC分类号： C12N15/09 , C07K14/34 , C12N1/21 , C12N15/00 , C12N15/31 , C12N15/63 , C12N15/77 , C12P13/04 , C12P13/08 , C12P21/02 , C12R1/15 , C12P21/06 , C07H21/04 , C07K14/00 , C12N1/20 , C12N5/00
摘要： The invention pertains to a process for the microbial production of amino acids. The process involves boosting the export carrier activity and/or export gene expression of a microorganism which produces the desired amino acid. It was found that a single specific gene is responsible for the export of a given amino acid, and on that basis, a process for the microbial production of amino acids, involving the controlled boosting of the export gene expression and/or export carrier activity of a microorganism was developed, which produces the amino acid. The boosted expression or activity of the export carrier resulting from this process increases the secretion rate and thus increases transport of the desired amino acid.
摘要翻译： 本发明涉及一种微生物生产氨基酸的方法。 该方法包括增加产生所需氨基酸的微生物的出口载体活性和/或导出基因表达。 已经发现，单个特定基因负责输出给定的氨基酸，并且在此基础上，氨基酸的微生物生产过程涉及出口基因表达的受控增强和/或导出载体活性 开发出产生氨基酸的微生物。 由该方法产生的出口载体的增强的表达或活性增加分泌速率，从而增加所需氨基酸的转运。
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2.发明授权Nucleotide sequences of coryneform bacteria coded for proteins participating in L-serine metabolism and method for microbial production of L-serine 失效编码参与L-丝氨酸代谢的蛋白质的棒状细菌的核苷酸序列和用于L-丝氨酸的微生物生产的方法公开(公告)号：US08119374B2
公开(公告)日：2012-02-21
申请号：US12136190
申请日：2008-06-10
摘要： This invention relates to the nucleotide sequence of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase activity. The invention also relates to microorganisms used in methods for producing L-serine.
摘要翻译： 本发明涉及编码与L-丝氨酸脱水酶活性降低和关闭的L-丝氨酸代谢相关的蛋白质的棒状细菌的核苷酸序列。 本发明还涉及用于生产L-丝氨酸的方法中使用的微生物。
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3.发明申请L-LYSINE-PRODUCING CORYNEBACTERIA AND PROCESS FOR THE PREPARATION OF L-LYSINE 有权生产L-赖氨酸的L-赖氨酸和L-赖氨酸的制备方法公开(公告)号：US20100261233A1
公开(公告)日：2010-10-14
申请号：US12249092
申请日：2008-10-10
申请人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
发明人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
IPC分类号： C12P13/08
摘要： L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
摘要翻译： 具有增强的lysE基因（赖氨酸出口载体基因）的L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因）的lys附加基因，lysC基因（天冬氨酸激酶基因），dapB基因 （二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）增强，特别是过表达，以及制备L-赖氨酸的方法。
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4.发明申请公开(公告)号：US20080153139A1
公开(公告)日：2008-06-26
申请号：US11663984
申请日：2006-04-06
申请人： Jan Marienhagen , Lothar Eggeling , Hermann Sahm
发明人： Jan Marienhagen , Lothar Eggeling , Hermann Sahm
CPC分类号： C12P13/08 , C12N9/1096
摘要： The invention relates to a method for producing L-valine and to a suitable microorganism. The inventive method is characterized by preferably enhancing the transaminase C activity of a coryneform bacterium, especially Corynebacteriuim glutamicum. The organisms so modified have a yield in L-valine which is 35.8% higher than that of non-modified organisms.
摘要翻译： 本发明涉及L-缬氨酸和合适微生物的生产方法。 本发明的方法的特征在于优选增强棒状细菌，特别是谷氨酸棒杆菌（Corynebacteriuim glutamicum）的转氨酶C活性。 如此修饰的生物体在L-缬氨酸中的产率高于非改性生物体的35.8％。
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5.发明申请L-LYSINE-PRODUCING CORYNEBACTERIA AND PROCESS FOR THE PREPARATION OF L-LYSINE 有权生产L-赖氨酸的L-赖氨酸和L-赖氨酸的制备方法公开(公告)号：US20070054380A1
公开(公告)日：2007-03-08
申请号：US11425091
申请日：2006-06-19
申请人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
发明人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
摘要翻译： 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。
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6.发明授权L-Lysine-producing corynebacteria and process for the preparation of L-lysine 有权L-赖氨酸生产性棒状杆菌和L-赖氨酸的制备方法公开(公告)号：US07094584B2
公开(公告)日：2006-08-22
申请号：US10804120
申请日：2004-03-19
申请人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Möckel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
发明人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Möckel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
IPC分类号： C12P13/08
摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
摘要翻译： 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。
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7.发明授权Nucleotide sequences coding for the thrE gene and process for the enzymatic production of L-threonine using coryneform bacteria 失效编码thrE基因的核苷酸序列和使用棒状细菌酶促生产L-苏氨酸的方法公开(公告)号：US06913909B2
公开(公告)日：2005-07-05
申请号：US09963521
申请日：2001-09-27
申请人： Petra Ziegler , Lothar Eggeling , Hermann Sahm , Georg Thierbach
发明人： Petra Ziegler , Lothar Eggeling , Hermann Sahm , Georg Thierbach
IPC分类号： C12N15/09 , C07K14/34 , C12N1/21 , C12N15/01 , C12N15/31 , C12N15/77 , C12P13/08 , C12R1/15 , C12N1/20 , C12N15/00 , C12P21/06 , C12Q1/48
摘要： The invention relates to preferably recombinant DNA derived from Corynebacterium and replicable in coryneform microorganisms, which contains at least one nucleotide sequence that codes for the thrE gene, and a process for the production of L-threonine, which is characterised in that the following steps are carried out: a) Fermentation of microorganisms in which at least the thrE gene is amplified (overexpressed), optionally in combination with further genes, b) Enrichment of the L-threonine in the medium or in the cells of the microorganisms, and c) Isolation of the L-threonine.
摘要翻译： 本发明涉及优选来源于棒状杆菌的重组DNA，并可在包含编码thrE基因的至少一个核苷酸序列的棒状细菌中复制，以及L-苏氨酸的制备方法，其特征在于以下步骤为 进行：a）至少将thrE基因扩增（过表达）的微生物发酵，任选与其它基因组合，b）在培养基中或微生物细胞中富集L-苏氨酸，和c） L-苏氨酸的分离。
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公开(公告)号：US06562607B2
公开(公告)日：2003-05-13
申请号：US09848726
申请日：2001-05-04
IPC分类号： C12N900
CPC分类号： C12N9/1288 , C12P13/14
摘要： This invention relates to a genetically modified coryneform bacterium, the cls gene of which is amplified, and to an isolated polynucleotide, which codes for cardiolipin synthase from coryneform bacteria and to a process for the fermentative production of L-amino acids with amplification of the cls gene in the bacteria and to the use of the polynucleotide as a primer or hybridization probe.
摘要翻译： 本发明涉及基因修饰的棒状细菌，其cls基因被扩增，并且分离的多核苷酸编码来自棒状细菌的心磷脂合成酶，以及用于扩增cls的L-氨基酸的发酵生产方法 基因和使用多核苷酸作为引物或杂交探针。
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9.发明授权Production of L-isoleucine by means of recombinant microorganisms with deregulated threonine dehydratase 失效通过具有失调的苏氨酸脱水酶的重组微生物生产L-异亮氨酸
公开(公告)号：US6107063A
公开(公告)日：2000-08-22
申请号：US669378
申请日：1997-03-20
申请人： Bettina Moeckel , Lothar Eggeling , Hermann Sahm
发明人： Bettina Moeckel , Lothar Eggeling , Hermann Sahm
IPC分类号： C12N15/09 , C12N1/21 , C12N9/88 , C12N15/00 , C12N15/60 , C12P13/06 , C12R1/15 , C12N15/31 , C12N15/63
摘要： The invention relates to processes for the microbial production of L-isoleucine. To this end, in a gene in vitro of a threonine dehydratse, one or more bases in the gene region coding the enzyme's allosteric domains are exchanged in such a way that at least one amino acid in the amino acid sequence of the allosteric domains of the enzyme is replaced by another so that the enzyme is no longer inhibited by L-isoleucine feedback. Furthermore, concrete amino acid exchanges in the amino acid sequence of the enzyme are effected in a gene in vitro of a threonin dehydratase of Corynebacterium glutamicum by base exchange both outside and inside and outside the gene region coding the allosteric domains of the enzyme si that, after the transformation of such mutated threonine dehydratase genes into a threonine or L-isoleucine-producing host cell, the latter repeatedly forms L-isoleucine.
摘要翻译： PCT No.PCT / DE95 / 00017 Sec。 371日期1997年3月20日 102（e）1997年3月20日PCT PCT 1995年1月9日PCT公布。 WO95 / 19442 PCT公开号 日期1995年7月20日本发明涉及L-异亮氨酸的微生物生产方法。 为此，在苏氨酸脱水体外的基因的基因中，编码酶变构域的基因区域中的一个或多个碱基以这样的方式交换，使得至少一个氨基酸序列的变构域 酶被另一个替代，使得酶不再被L-异亮氨酸反馈抑制。 此外，酶的氨基酸序列中的具体氨基酸交换通过在编码酶si的变构结构域的基因区域的外部和外部的碱基交换体外在谷氨酸棒杆菌的苏氨酸脱水酶的基因中进行， 在将这种突变的苏氨酸脱水酶基因转化成产生苏氨酸或L-异亮氨酸的宿主细胞后，后者重复形成L-异亮氨酸。
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10.发明授权Nucleotide sequences coding for proteins involved in the biosynthesis of L-serine, an improved method for the microbial production of L-serine and a genetically modified microorganism suitable therefor 失效编码参与L-丝氨酸生物合成的蛋白质的核苷酸序列，用于L-丝氨酸微生物生产的改进方法和适用于其的转基因微生物公开(公告)号：US07083952B2
公开(公告)日：2006-08-01
申请号：US10224574
申请日：2002-08-21
CPC分类号： C12P13/06 , C12N9/1096 , C12N9/16
摘要： The present invention relates to nucleotide sequences of coryneform bacteria, coding for proteins involved in the bio-synthesis of L-serine and to methods for the isolation thereof. The invention further relates to an improved method for the production of L-serine. In addition, the present invention relates to the use of L-serine in the food, animal feed and/or pharmaceutical industries or in human medicine.
摘要翻译： 本发明涉及编码参与L-丝氨酸的生物合成的蛋白质的棒状细菌的核苷酸序列及其分离方法。 本发明还涉及一种生产L-丝氨酸的改进方法。 此外，本发明涉及L-丝氨酸在食品，动物饲料和/或制药工业中或在人类药物中的用途。
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