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    Microfluidic affinity assays with improved performance
    1.
    发明申请
    Microfluidic affinity assays with improved performance 审中-公开
    具有改善性能的微流控亲和测定

    公开(公告)号：US20060246526A1

    公开(公告)日：2006-11-02

    申请号：US11292996

    申请日：2005-12-02

    申请人： Mats Inganas ,  Magnus Ljungstrom

    发明人： Mats Inganas ,  Magnus Ljungstrom

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54366 ,  G01N33/581

    摘要： Method for measuring the signal from a label in a labeled measuring reagent that has been specifically adsorbed to its affinity counterpart in a zone of a porous nanolitre (nl) bed that is present in a microchannel structure of a microfluidic device. The measuring is part of an assay for determining an analyte of a sample by performing one, two or more heterogeneous specific affinity reactions which comprises that said labeled measuring reagent becomes affinity bound to said zone.

    摘要翻译： 用于测量来自在微流体装置的微通道结构中存在的多孔纳尼特（nl）床的区域中已特异性吸附到其亲和对应物的标记测量试剂中的标记的信号的方法。 测量是通过进行一种，两种或更多种非均相特异性亲和反应来测定样品分析物的测定的一部分，其包括所述标记的测量试剂变得亲和结合到所述区域。

    VERSATILE FLOW PATH
    2.
    发明申请
    VERSATILE FLOW PATH 审中-公开
    VERSATILE流程

    公开(公告)号：US20090010819A1

    公开(公告)日：2009-01-08

    申请号：US11815959

    申请日：2006-01-17

    申请人： Mats Holmquist ,  Mats Inganas ,  Gerald Jesson ,  Magnus Ljungstrom

    发明人： Mats Holmquist ,  Mats Inganas ,  Gerald Jesson ,  Magnus Ljungstrom

    IPC分类号： B01J19/00

    CPC分类号： B01F17/0028 ,  B01F17/005 ,  B01F17/0064 ,  B01J2219/00605 ,  B01J2219/00626 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/5027 ,  B01L3/502707 ,  B01L2300/0636 ,  G01N27/44752 ,  G01N33/54366

    摘要： A flow path comprising a reaction cavity in which there is a solid phase to which an affinity reactant I1 has been immobilized by the use of an immobilizing pair of reactive structures that comprises a) a plurality of functional equal structures RSsp on the solid phase, and b) a structure RSari on affinity reactant I1, where RSsp and RSari are mutually reactive with each other to the formation of a link structure that immobilizes affinity reactant I1 to the solid phase. The characteristic feature is that the solid phase comprises a plurality of structures that derive from RSsp but do not immobilize affinity reactant I1 to the solid phase.

    摘要翻译： 一种流路，其包含反应腔，其中存在通过使用固定的一对反应结构固定亲和反应物I1的固相，其包含a）在固相上的多个功能相同的结构RSsp，以及 b）亲和反应物I1的结构RSari，其中RSsp和RSari彼此相互反应以形成将亲和反应物I1固定在固相上的连接结构。 其特征在于，固相包含从RSsp衍生但不将亲和反应物I1固定在固相上的多个结构。

    Sequential Method
    3.
    发明申请
    Sequential Method 审中-公开
    顺序方法

    公开(公告)号：US20090215196A1

    公开(公告)日：2009-08-27

    申请号：US11572316

    申请日：2005-07-15

    申请人： Bo Ek ,  Mats Holmquist ,  Karolina Osterlund ,  Mats Inganas

    发明人： Bo Ek ,  Mats Holmquist ,  Karolina Osterlund ,  Mats Inganas

    IPC分类号： G01N33/566

    CPC分类号： G01N33/54306 ,  B01L3/5027 ,  G01N33/54366

    摘要： A method for determination of an analyte in an original liquid sample by performing an inhibition affinity assay in a microchannel structure of a microfluidic device. The main characteristic feature is the steps of: (i) providing the microfluidic device in a form where the microchannel structure comprises a reaction microcavity which a) an inlet end, and b) contains a solid phase with binding sites (BS) for an analyte or an analyte-related entity, (ii) providing a liquid sample (1) containing the analyte or the analyte-related entity within the microchannel structure at the inlet end of the reaction microcavity, and flowing sample (1) through the reaction microcavity under conditions such that the analyte or the analyte-related entity is captured by BS leaving a portion of BS unoccupied (free BS), (iii) providing a liquid sample (2) which contains an analytically detectable analogue of the analyte or of the analyte-related entity, and flowing sample (2) through said reaction microcavity to capture the analogue by free BS, and (iv) measuring the amount of analyte analogue captured in step (iii) and relating this amount to the amount of analyte in an original sample.

    摘要翻译： 一种通过在微流体装置的微通道结构中进行抑制亲和力测定来测定原始液体样品中的分析物的方法。 主要特征是以下步骤：（i）以微通道结构包含反应微腔的形式提供微流体装置，其中a）入口端和b）包含具有用于分析物的结合位点（BS）的固相 或分析物相关实体，（ii）在反应微腔的入口端处提供在微通道结构内含有分析物或分析物相关实体的液体样品（1），并将样品（1）流过反应微腔下 条件，使得分析物或分析物相关实体被BS捕获，留下一部分BS未占用（自由BS），（iii）提供含有分析物或分析物 - 分析物的可分析检测的类似物的液体样品（2） 相关实体和流动样品（2）通过所述反应微腔以通过游离BS捕获类似物，和（iv）测量步骤（iii）中捕获的分析物类似物的量，并将该量与在 原始样本

    Grading of Immune Responses
    5.
    发明申请
    Grading of Immune Responses 审中-公开
    免疫反应分级

    公开(公告)号：US20080280378A1

    公开(公告)日：2008-11-13

    申请号：US11572313

    申请日：2005-07-15

    申请人： Mats Inganas

    发明人： Mats Inganas

    IPC分类号： G01N33/543

    CPC分类号： G01N33/54306 ,  B01L3/5027 ,  G01N33/54366

    摘要： The invention is a method for grading a polyclonal antibody response with respect to a desired 5 binding specificity. The response is provoked by an antigenic agent in a host, preferably a vertebrate host. The characteristic feature of the method comprises the steps of: (i) providing a reaction microcavity having an inlet end and an outlet end and containing a solid phase that exposes an immobilized binding structure (BS) that are capable of affinity binding antibodies (Ab) of the response, (ii) flowing a liquid sample containing antibodies of the response in the direction from the inlet end to the outlet end through the solid phase, (iii) determining the distribution of antibodies, which are captured during step (ii), along the flow direction of the solid phase, and (iv) grading the response based on the distribution determined in step (iii).

    摘要翻译： 本发明是相对于期望的5结合特异性对多克隆抗体应答进行分级的方法。 该反应由宿主，优选脊椎动物宿主中的抗原试剂引发。 该方法的特征包括以下步骤：（i）提供具有入口端和出口端并包含固相的反应微腔，所述固相暴露出能够结合抗体（Ab）的固定化结合结构（BS） （ii）使包含响应的抗体的液体样品在从入口端到出口端的方向流过固相，（iii）确定在步骤（ii）期间捕获的抗体的分布， 沿着固相的流动方向，以及（iv）基于步骤（iii）中确定的分布来对响应进行分级。

    Flow Paths Comprising One or Two Porous Beds
    6.
    发明申请
    Flow Paths Comprising One or Two Porous Beds 有权
    包含一个或两个多孔床的流路

    公开(公告)号：US20070241061A1

    公开(公告)日：2007-10-18

    申请号：US10587428

    申请日：2005-01-31

    申请人： Johan Engstrom ,  Mats Inganas ,  Gunnar Thorsen

    发明人： Johan Engstrom ,  Mats Inganas ,  Gunnar Thorsen

    IPC分类号： B01J8/02 ,  C02F1/68

    CPC分类号： B01L3/502753 ,  B01L3/5023 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/087 ,  G01N30/461 ,  G01N30/6095 ,  Y10T436/25125

    摘要： A microfluidic device that comprises a microchannel structure in which there are one, two or more flow paths (101;201a,b;301a,a′,b) all of which comprises a porous bed I (104,204,304) that is common for all of the flow paths and exposes an immobilized reactant R that is capable of interacting with a solute S that passes through the bed. The characteristics are that at least one of the flow paths comprises/comprise a second porous bed II (105,205,305) that is placed upstream of porous bed I (104,204,304) and is dummy with respect to interaction with solute S but capable of interacting with a substance DS that is present in a liquid aliquot together with solute S and is capable of disturbing the result of the interaction between solute S and said immobilized reactant R. There is also disclosed a method utilizing the device and variant of the device in which the immobilized R is replaced with a generic affinity ligand LI and/or porous bed II exposes a generic ligand LII that may be different from LI.

    摘要翻译： 一种微流体装置，其包括微通道结构，其中存在一个，两个或更多个流动路径（101; 201a，b; 301a，a'，b），所述流路包括多孔床I（104,204,304） 所有流动路径并暴露出能够与通过床的溶质S相互作用的固定化反应物R. 特征是至少一个流动路径包括/包括放置在多孔床I（104,204,304）上游的第二多孔床II（105,205,305），并且相对于与溶质S的相互作用但是能够与物质相互作用的虚拟 DS与溶质S一起存在于液体等分试样中，并且能够干扰溶质S和所述固定化反应物R之间的相互作用的结果。还公开了利用其中固定化R的装置和变体的方法 被一般亲和配体L 1 N替代和/或多孔床II暴露可能不同于L I的通用配体L II II。

    Confidence determination
    7.
    发明申请
    Confidence determination 有权
    信心决心

    公开(公告)号：US20070118490A1

    公开(公告)日：2007-05-24

    申请号：US10555009

    申请日：2004-06-30

    申请人： Tobias Soderman ,  Mats Inganas

    发明人： Tobias Soderman ,  Mats Inganas

    IPC分类号： G06F15/18 ,  G06E1/00

    CPC分类号： G06T7/0004 ,  B01L3/5027 ,  G06N3/02 ,  G06T7/0012 ,  G06T2207/10008 ,  G06T2207/20084

    摘要： The present invention relates to a method, a system, a neural network and a computer program product for determining the quality of an analytical process, preferably the confidence value C. The analytical process is performed in a microchannel structure of a microfluidic device, from which data information of the analytic process is acquired by scanning at least one search area of the microchannel structure for signal data. Said search area comprises the result of the analytical process and said acquired data information is stored as an image, one image for each scanned search area.

    摘要翻译： 本发明涉及一种用于确定分析过程质量，优选置信度值C的方法，系统，神经网络和计算机程序产品。分析过程在微流体装置的微通道结构中进行， 通过扫描用于信号数据的微通道结构的至少一个搜索区域来获取分析处理的数据信息。 所述搜索区域包括分析处理的结果，并且所述获取的数据信息被存储为图像，每个扫描的搜索区域存储一个图像。