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    Microfluidic devices and systems
    1.
    发明授权
    Microfluidic devices and systems 有权

    公开(公告)号：US10247655B2

    公开(公告)日：2019-04-02

    申请号：US14907867

    申请日：2014-07-30

    Applicant: Sphere Fluidics Limited

    Inventor： Clive A. Smith ,  Xin Li ,  Karine Enesa ,  Xin Liu ,  Frank F. Craig ,  Graeme Whyte

    IPC: G01N15/14 ,  B01L3/00 ,  C12Q1/04 ,  C12Q1/06 ,  G01N21/49 ,  G01N15/00

    Abstract: A method of label-free cell or particle sorting in a microfluidic device includes providing a stream of aqueous droplets in oil in a channel of the microfluidic device, wherein at least some of the droplets include cells or particles and illuminating the stream from a first direction. Then, one detects scattered light from cells or particles within said aqueous droplets in a second direction. Next, one determines a number of the cells or particles in each droplet from the scattered light and sorts the aqueous droplets into differentiated streams. Notably, the refractive index of said oil is modified to closely match a refractive index of the aqueous droplets to reduce light scattered from boundaries of the droplets. A volume of the droplets can also controlled.

    SYSTEM AND METHODS
    5.
    发明申请
    SYSTEM AND METHODS 有权

    公开(公告)号：US20210308682A1

    公开(公告)日：2021-10-07

    申请号：US17349068

    申请日：2021-06-16

    Applicant: SPHERE FLUIDICS LIMITED

    Inventor： Frank F. CRAIG ,  Marian Rehak ,  David Holmes ,  Clive A. Smith ,  Xin Liu ,  Giuseppe Benazzi ,  Xin Li ,  Vinayaka Pawate ,  Robert Frank Marchington ,  Richard Francis Day ,  Michael Stewart Hazell

    IPC: B01L3/00 ,  G01N35/08 ,  G01N15/14 ,  C12M1/00

    Abstract: We describe instruments, and cartridges therefor, for processing droplets of water in oil-in-water emulsions, the droplets containing biological entities such as cells. In one embodiment a method of such processing comprises: providing a plurality of the entities in a fluid: preparing a droplet from said fluid: determining whether said droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of said determination; and dispensing said sorted droplet into a reservoir, wherein said dispensing comprises: identifying said sorted droplet for dispensing; extracting said sorted droplet from a first fluidic flow path of said fluid by transferring said sorted droplet from said first fluidic flow path into a second fluidic flow path; and ejecting said sorted droplet from said second fluidic flow path into said reservoir by applying pressure to said second fluidic flow path. In preferred embodiments the droplet contents are tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and afterwards retrieved for further processing or analysis, or for subsequent use, for example in fermenting or the like.

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