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    Method for culturing a microorganism and promoting microbial growth and metabolism
    1.
    发明授权
    Method for culturing a microorganism and promoting microbial growth and metabolism 失效
    培养微生物并促进微生物生长和代谢的方法

    公开(公告)号：US06660516B1

    公开(公告)日：2003-12-09

    申请号：US09025424

    申请日：1998-02-18

    申请人： Takeshi Imamura ,  Yasutsugu Yamada ,  Tetsuya Yano ,  Akira Kuriyama ,  Shinya Kozaki

    发明人： Takeshi Imamura ,  Yasutsugu Yamada ,  Tetsuya Yano ,  Akira Kuriyama ,  Shinya Kozaki

    IPC分类号： B09B300

    CPC分类号： C12N1/38 ,  C02F1/4618 ,  C02F3/34 ,  C02F2209/04 ,  C02F2209/06 ,  C12R1/19 ,  Y10S435/822 ,  Y10S435/849

    摘要： A method is provided for culturing a microorganism in a culture medium containing a carbon source and an electrolyzed water containing not more than 0.4 ppm chlorine produced during electrolyzing water containing an electrolyte in an electrolytic cell. The culture medium may also contain a carbon source and an acidic water having a pH value of 1-4 and a redox potential from 800 mV to 1500 mV. The acidic water is obtained by electrolysis of water in an electrolytic cell. Further, the culture medium may contain a carbon source and an alkaline water having a pH value of 10-13 and a redox potential from −1000 mV to 800 mV. The alkaline water is obtained by electrolysis of water in an electrolytic cell. The redox potential for the alkaline water is determined by the use of a platinum electrode as a working electrode and a silver-silver chloride electrode as a reference electrode. The microorganism may be selected from Escherichia coli, strain J1, strain JM2N, or an artificial recombinant. The microorganism can decompose a pollutant, such as phenol, toluene, cresol, trichlorethylene and dichloroethylene.

    摘要翻译： 提供了一种在含有碳源的培养基中培养微生物的方法，以及在电解槽中含有电解质中含有电解质的水中产生的含有不超过0.4ppm氯的电解水。 培养基也可以含有碳源和pH值为1-4，氧化还原电位为800mV〜1500mV的酸性水。 酸性水通过在电解池中电解水而获得。 此外，培养基可以含有碳源和pH值为10-13，氧化还原电位为-1000mV至800mV的碱性水。 碱性水通过在电解槽中电解电解而获得。 碱性水的氧化还原电位通过使用铂电极作为工作电极和银 - 氯化银电极作为参比电极来确定。 微生物可以选自大肠杆菌，菌株J1，菌株JM2N，或人工重组体。 微生物可以分解苯酚，甲苯，甲酚，三氯乙烯和二氯乙烯等污染物。

    Polyhydroxyalkanoate copolymer including unit having bromo group in side chain and method for producing the same
    4.
    发明授权
    Polyhydroxyalkanoate copolymer including unit having bromo group in side chain and method for producing the same 失效
    聚羟基链烷酸酯共聚物，包括在侧链中具有溴基的单元及其制备方法

    公开(公告)号：US07135540B2

    公开(公告)日：2006-11-14

    申请号：US10359600

    申请日：2003-02-07

    申请人： Tsutomu Honma ,  Shinya Kozaki ,  Takeshi Imamura ,  Takashi Kenmoku ,  Tatsuki Fukui ,  Etsuko Sugawa ,  Tetsuya Yano

    发明人： Tsutomu Honma ,  Shinya Kozaki ,  Takeshi Imamura ,  Takashi Kenmoku ,  Tatsuki Fukui ,  Etsuko Sugawa ,  Tetsuya Yano

    IPC分类号： C08G63/02

    CPC分类号： C12P7/625 ,  C08G63/6822 ,  C08G63/6882 ,  C12P11/00

    摘要： The invention provides a polyhydroxyalkanoate having a bromo group in a unit and being thermally stable and capable of arbitrarily controlling physical properties, and a producing method thereof. According to the invention, there are provided a polyhydroxyalkanoate copolymer including a 3-hydroxy-ω-bromoalkanoic acid unit represented by a formula—[OCH((CH2)nBr)CH2C(O)]— (n=1 to 8) (1) and a unit represented by a formula —[OCH((CH2)mR)CH2C(O)]— (2) within a same molecule, and a method for producing a polyhydroxyalkanoate copolymer by microorganisms, utilizing a ω-bromoalkanoic acid represented by a formula Br(CH2)pCH2CH2COOH (p=1 to 8) (20) and a compound represented by a formula R(CH2)qCH2CH2COOH (21) as raw materials.

    摘要翻译： 本发明提供一种具有溴基团并且是热稳定的并能够任意控制物理性能的聚羟基链烷酸酯及其制备方法。 根据本发明，提供了一种包含由式 - [OCH（（CH 2）n）n Br表示的3-羟基-ω-溴代链烷酸单元的聚羟基链烷酸酯共聚物 ）CH 2 C（O）] - （n = 1-8）（1）和由式 - [OCH（（CH 2）2） （O）] - （2），以及通过微生物制备聚羟基链烷酸酯共聚物的方法，使用由以下化合物表示的ω-溴烷酸： 式（Br 2 CH 2 CH 2 CH 2 CH 2 CH 2 CH 2 CH 2 COOH（p = 1〜8）（20 ）和由式R（CH 2 CH 2）q CH 2 CH 2 COOH（21）表示的化合物 作为原料。