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    Nuclease Inhibitor Cocktail
    2.
    发明申请
    Nuclease Inhibitor Cocktail 有权
    核酸酶抑制剂鸡尾酒

    公开(公告)号：US20070148174A1

    公开(公告)日：2007-06-28

    申请号：US11567651

    申请日：2006-12-06

    申请人： W. Kudlicki ,  Matthew Winkler ,  Brittan Pasloske

    发明人： W. Kudlicki ,  Matthew Winkler ,  Brittan Pasloske

    IPC分类号： A61K39/395 ,  C12Q1/68

    CPC分类号： C12N15/1003 ,  C07K16/40 ,  C12N15/1096 ,  C12Q1/34 ,  G01N33/573 ,  G01N2333/922

    摘要： Methods and compositions for inhibiting and/or inactivating nucleases by employing nuclease inhibitors are provided. The nuclease inhibitors comprise anti-nuclease antibodies and non-antibody nuclease inhibitors. The anti-nuclease antibodies of the present invention may be a polyclonal or monoclonal antibodies, and may be anti-ribonuclease antibodies, anti-deoxyribonuclease antibodies, or antibodies to non-specific nucleases. A preferred embodiment comprises at least two nuclease inhibitors, and is referred to as a nuclease inhibitor cocktail. In some specific embodiments, the invention concerns methods of performing in vitro translation comprising obtaining a first nuclease inhibitor, which inhibitor is further defined as an anti-nuclease antibody, and placing the anti-nuclease antibody in an in vitro translation reaction. In many cases, the in vitro translation reaction comprises at least one nuclease, which may be a ribonuclease, a deoxyribonuclease, or a nonspecific nuclease. The invention also relates to kits for the performance of various microbiological procedures, which kits comprise the nuclease inhibitors described herein.

    摘要翻译： 提供了通过使用核酸酶抑制剂抑制和/或灭活核酸酶的方法和组合物。 核酸酶抑制剂包括抗核酸酶抗体和非抗体核酸酶抑制剂。 本发明的抗核酸酶抗体可以是多克隆或单克隆抗体，并且可以是抗核糖核酸酶抗体，抗脱氧核糖核酸酶抗体或对非特异性核酸酶的抗体。 优选的实施方案包括至少两种核酸酶抑制剂，并且被称为核酸酶抑制剂混合物。 在一些具体实施方案中，本发明涉及进行体外翻译的方法，包括获得第一核酸酶抑制剂，该抑制剂被进一步定义为抗核酸酶抗体，并将抗核酸酶抗体置于体外翻译反应中。 在许多情况下，体外翻译反应包含至少一个核酸酶，其可以是核糖核酸酶，脱氧核糖核酸酶或非特异性核酸酶。 本发明还涉及用于执行各种微生物程序的试剂盒，该试剂盒包含本文所述的核酸酶抑制剂。

    Nuclease inhibitor cocktail
    3.
    发明申请
    Nuclease inhibitor cocktail 有权
    核酸酶抑制剂鸡尾酒

    公开(公告)号：US20050026284A1

    公开(公告)日：2005-02-03

    申请号：US10675860

    申请日：2003-09-30

    申请人： W. Kudlicki ,  Matthew Winkler ,  Brittan Pasloske

    发明人： W. Kudlicki ,  Matthew Winkler ,  Brittan Pasloske

    IPC分类号： C07K16/40 ,  C12N15/10 ,  C12Q1/34 ,  G01N33/573 ,  A61K48/00 ,  A61K39/395 ,  C12N15/85

    CPC分类号： C12N15/1003 ,  C07K16/40 ,  C12N15/1096 ,  C12Q1/34 ,  G01N33/573 ,  G01N2333/922

    摘要： Methods and compositions for inhibiting and/or inactivating nucleases by employing nuclease inhibitors are provided. The nuclease inhibitors comprise anti-nuclease antibodies and non-antibody nuclease inhibitors. The anti-nuclease antibodies of the present invention may be a polyclonal or monoclonal antibodies, and may be anti-ribonuclease antibodies, anti-deoxyribonuclease antibodies, or antibodies to non-specific nucleases. A preferred embodiment comprises at least two nuclease inhibitors, and is referred to as a nuclease inhibitor cocktail. In some specific embodiments, the invention concerns methods of performing in vitro translation comprising obtaining a first nuclease inhibitor, which inhibitor is further defined as an anti-nuclease antibody, and placing the anti-nuclease antibody in an in vitro translation reaction. In many cases, the in vitro translation reaction comprises at least one nuclease, which may be a ribonuclease, a deoxyribonuclease, or a nonspecific nuclease. The invention also relates to kits for the performance of various microbiological procedures, which kits comprise the nuclease inhibitors described herein.

    摘要翻译： 提供了通过使用核酸酶抑制剂抑制和/或灭活核酸酶的方法和组合物。 核酸酶抑制剂包括抗核酸酶抗体和非抗体核酸酶抑制剂。 本发明的抗核酸酶抗体可以是多克隆或单克隆抗体，并且可以是抗核糖核酸酶抗体，抗脱氧核糖核酸酶抗体或对非特异性核酸酶的抗体。 优选的实施方案包括至少两种核酸酶抑制剂，并且被称为核酸酶抑制剂混合物。 在一些具体实施方案中，本发明涉及进行体外翻译的方法，包括获得第一核酸酶抑制剂，该抑制剂被进一步定义为抗核酸酶抗体，并将抗核酸酶抗体置于体外翻译反应中。 在许多情况下，体外翻译反应包含至少一个核酸酶，其可以是核糖核酸酶，脱氧核糖核酸酶或非特异性核酸酶。 本发明还涉及用于执行各种微生物程序的试剂盒，该试剂盒包含本文所述的核酸酶抑制剂。

    Enzymatic digestion of tissue
    6.
    发明申请
    Enzymatic digestion of tissue 审中-公开
    酶消化组织

    公开(公告)号：US20060188892A1

    公开(公告)日：2006-08-24

    申请号：US11076455

    申请日：2005-03-09

    申请人： Gary Latham ,  Brittan Pasloske ,  Heidi Peltier

    发明人： Gary Latham ,  Brittan Pasloske ,  Heidi Peltier

    IPC分类号： C12Q1/68 ,  C12N1/08

    CPC分类号： C12Q1/6806 ,  C12N15/1003

    摘要： The present invention concerns a compositions and method for isolating a nucleic acid from a cell-containing sample. There is disclosed a method comprising obtaining at least one cell-containing sample, which comprises a cell containing nucleic acid, obtaining at least one catabolic enzyme, obtaining at least one nuclease inhibitor, preparing an admixture of the sample, the catabolic enzyme, and the nuclease inhibitor, maintaining the admixture under conditions where the catabolic enzyme is active, and agitating the admixture, where the sample is digested to produce a nucleic acid-containing lysate of the sample.

    摘要翻译： 本发明涉及从含细胞的样品中分离核酸的组合物和方法。 公开了一种方法，包括获得至少一种含细胞的样品，其包含含有核酸的细胞，获得至少一种分解代谢酶，获得至少一种核酸酶抑制剂，制备样品，分解代谢酶和 核酸酶抑制剂，在分解代谢酶活性的条件下保持混合物，并搅拌混合物，其中样品被消化以产生样品的含核酸裂解物。

    Methods and compositions for preparing capped RNA
    7.
    发明申请
    Methods and compositions for preparing capped RNA 审中-公开
    用于制备加帽RNA的方法和组合物

    公开(公告)号：US20050287539A1

    公开(公告)日：2005-12-29

    申请号：US10880350

    申请日：2004-06-29

    申请人： Emmanuel Labourier ,  Brittan Pasloske

    发明人： Emmanuel Labourier ,  Brittan Pasloske

    IPC分类号： C12N15/79 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12N15/79

    摘要： The present invention concerns methods and compositions for increasing the yield of capped and full-length RNA transcripts produced in in vitro transcription reactions. Such methods and compositions can be used for cost-efficient, large scale production of capped full-length RNA transcripts that can be subsequently translated. Methods and compositions involve reaction conditions that promote such production, and includes the implementation of fed-batch introduction of GTP, which competes with a cap analog.

    摘要翻译： 本发明涉及用于增加在体外转录反应中产生的封端和全长RNA转录物的产量的方法和组合物。 这些方法和组合物可用于成本有效，大规模生产可随后翻译的封端全长RNA转录物。 方法和组合物涉及促进这种生产的反应条件，并且包括实施补料分批引入GTP，其与帽类似物竞争。

    Crude biological derivatives competent for nucleic acid detection
    10.
    发明申请
    Crude biological derivatives competent for nucleic acid detection 审中-公开
    用于核酸检测的粗生物衍生物

    公开(公告)号：US20050277121A1

    公开(公告)日：2005-12-15

    申请号：US10866525

    申请日：2004-06-11

    申请人： Brittan Pasloske ,  Xingwang Fang

    发明人： Brittan Pasloske ,  Xingwang Fang

    IPC分类号： C12N15/10 ,  C12Q1/68

    CPC分类号： C12N15/1096 ,  C12Q1/6806 ,  C12Q2527/119 ,  C12Q2521/119 ,  C12Q2521/107

    摘要： The invention relates generally to the fields of making biological unit lysates or admixtures of body fluids and of RNA analysis. More specifically, it relates to direct methods for the detection of a specific sequence of RNA in a biological unit, for example a virus, cell or tissue sample, or a body fluid, for example saliva, sputum, blood plasma, etc. More generally, the invention may be used to enzymatically manipulate and protect the RNA in lysate or bodily fluids for a number of applications.

    摘要翻译： 本发明一般涉及制造生物单元裂解物或体液的混合物和RNA分析的领域。 更具体地说，本发明涉及用于检测生物学单元例如病毒，细胞或组织样品或体液例如唾液，痰液，血浆等的特定序列RNA的直接方法。更一般地 ，本发明可用于在许多应用中酶促操纵和保护裂解物或体液中的RNA。