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124 条记录 (耗时 0.027 秒)

    Method of Identifying Sugar Chain Structure and Apparatus For Analyzing the Same
    91.
    发明申请
    Method of Identifying Sugar Chain Structure and Apparatus For Analyzing the Same 审中-公开
    识别糖链结构的方法及其分析方法

    公开(公告)号：US20080139396A1

    公开(公告)日：2008-06-12

    申请号：US10593157

    申请日：2005-03-18

    申请人： Akihiko Kameyama ,  Hisashi Narimatsu ,  Norihiro Kikuchi ,  Shuuichi Nakaya

    发明人： Akihiko Kameyama ,  Hisashi Narimatsu ,  Norihiro Kikuchi ,  Shuuichi Nakaya

    IPC分类号： C40B20/08

    CPC分类号： H01J49/0027

    摘要： An object of the present invention is to provide a system for analyzing a sugar chain structure which can determine a complete primary structure of a sugar chain simply and rapidly with a small amount of sample. In a method of identifying an analysis-objective sugar chain structure using a mass spectrometer by comparing a measured MS3 fragment pattern with a reference MS3 fragment pattern stored in a database, where the measured MS3 fragment pattern is a fragmentation pattern of each MS2 fragment ion included in a measured MS2 fragment pattern obtained by subjecting the analysis-objective sugar chain to a fragmentation mass spectroscopy, the present invention is characterized in that, among a plurality of MS2 fragment ions included in a measured MS2 fragment pattern, a fragmentation mass spectroscopy is performed on only selected MS2 fragment ions, where each of the selected fragmentations has a plurality of reference MS3 fragment patterns stored in a database whose mutual similarity index is smaller than a predetermined value, wherein the plurality of reference MS3 fragment patterns have the same precursor ion mass to charge ratio as that of the selected MS2 fragment ion.

    摘要翻译： 本发明的目的是提供一种用于分析糖链结构的系统，其可以用少量样品简单快速地确定糖链的完整的一级结构。 在使用质谱仪鉴定分析目标糖链结构的方法中，通过将测量的MS3片段模式与存储在数据库中的参考MS3片段模式进行比较，其中测量的MS3片段模式是包含每个MS2片段离子的片段化模式 在通过使分析目的糖链进行裂解质谱法测定的MS2片段图谱中，本发明的特征在于，在测定的MS2片段图案中包含的多个MS2片段离子中，进行碎片质谱法 在所选择的MS2片段离子上，其中每个选择的片段具有存储在相互相似性指数小于预定值的数据库中的多个参考MS3片段模式，其中多个参考MS3片段图案具有相同的前体离子质量 与所选MS2碎片离子的电荷比。

    DRUG SCREENING AND TARGET IDENTIFICATION USING SPR IMAGING OF TARGET PROTEIN ARRAYS
    93.
    发明申请
    DRUG SCREENING AND TARGET IDENTIFICATION USING SPR IMAGING OF TARGET PROTEIN ARRAYS 审中-公开
    使用目标蛋白质阵列的SPR成像进行药物筛选和目标鉴定

    公开(公告)号：US20080090734A1

    公开(公告)日：2008-04-17

    申请号：US11871078

    申请日：2007-10-11

    申请人： Hao XU ,  Feimeng Zhou

    发明人： Hao XU ,  Feimeng Zhou

    IPC分类号： C40B20/08

    CPC分类号： C40B20/08 ,  B01J2219/00725 ,  G01N33/6803 ,  G01N33/6845

    摘要： Drug screening and target identification using surface plasmon resonance imaging (SPRI) of a target protein array is provided. The Target Protein Array/SPRI system is used as parts of a strategy or method for label-free and real-time identification of a target protein or proteins of any inhibitor, agonist or other ligand. Methods that allow for rapidly classifying the mechanism of action of any inhibitor, agonist or other ligand; and for simultaneous identification and purification of an active ingredient of a natural product extract, which acts specifically on a target protein or proteins are provided. Techniques that can be used to prioritize inhibitors, agonists or ligands for lead compound development; to determine the therapeutic index of drug candidates; to assess the range of target proteins of drugs or drug candidates or lead compounds in organisms; and to discover novel target proteins of known drugs or known inhibitors are described.

    摘要翻译： 提供使用靶蛋白阵列的表面等离子体共振成像（SPRI）进行药物筛选和靶标鉴定。 靶蛋白阵列/ SPRI系统被用作无标记和实时鉴定任何抑制剂，激动剂或其它配体的靶蛋白或蛋白质的策略或方法的一部分。 允许快速分类任何抑制剂，激动剂或其他配体的作用机制的方法; 并且提供特异性作用于靶蛋白或蛋白质的天然产物提取物的活性成分的同时鉴定和纯化。 可用于优先使用抑制剂，激动剂或配体进行铅化合物开发的技术; 确定候选药物的治疗指标; 评估生物体内药物或药物候选物或铅化合物的靶蛋白范围; 并发现已知药物或已知抑制剂的新型靶蛋白。

    APPARATUS, COMPOSITIONS, AND METHODS FOR ASSESSMENT OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE PROGRESSION AMONG RAPID AND SLOW DECLINE CONDITIONS
    94.
    发明申请
    APPARATUS, COMPOSITIONS, AND METHODS FOR ASSESSMENT OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE PROGRESSION AMONG RAPID AND SLOW DECLINE CONDITIONS 有权
    用于评估慢性阻塞性慢性阻塞性慢性疾病进展的装置，组合物和方法

    公开(公告)号：US20080026949A1

    公开(公告)日：2008-01-31

    申请号：US11770074

    申请日：2007-06-28

    申请人： John Hoidal ,  Mary Scholand ,  Mark Leppert ,  Michael Paul ,  Robert Gritz ,  Joel Pounds ,  Richard Smith

    发明人： John Hoidal ,  Mary Scholand ,  Mark Leppert ,  Michael Paul ,  Robert Gritz ,  Joel Pounds ,  Richard Smith

    IPC分类号： C40B20/08 ,  C40B30/00 ,  C40B40/10

    CPC分类号： C12Q1/6883 ,  G01N33/6848 ,  G01N2800/122 ,  G06F19/18

    摘要： Methods are disclosed for generating and isolating an informative content repository of respiratory related biomarkers to accurately determine whether an individual has normal or abnormal pulmonary function. Specifically, methods are directed to determination of whether individuals have chronic obstructive pulmonary disease, and if so, whether the affected individuals experience rapid long decline or slow lung decline as a result of COPD. Also disclosed is an informative content repository of chronic obstructive pulmonary disease biomarkers, which when linked with other informative content provides a powerful tool for diagnosis, study, therapeutic discovery and development, condition management, health maintenance, and linking chronic obstructive pulmonary disease through pattern of life style, environmental exposure, and genetic susceptibility and inheritance. Disclosed herein is a chronic obstructive pulmonary disease biomarker informative content repository comprising at least one COPD biomarker, apparatus and methods to diagnose, assess, address, and ameliorate related conditions.

    摘要翻译： 公开了用于产生和分离呼吸相关生物标志物的信息内容库的方法，以准确地确定个体是否具有正常或异常的肺功能。 具体来说，方法是针对确定个体是否患有慢性阻塞性肺疾病，如果是，受影响的个体是否经历COPD的快速长期衰退或肺缓慢缓慢。 还披露了慢性阻塞性肺疾病生物标志物的信息内容库，当与其他信息内容联系时，通过模式的方式为诊断，研究，治疗发现和发展，病症管理，健康维持和慢性阻塞性肺疾病连接提供了强大的工具 生活方式，环境暴露和遗传易感性和遗传。 本文公开了一种慢性阻塞性肺疾病生物标志物信息内容库，其包含至少一种COPD生物标志物，用于诊断，评估，解决和改善相关病症的装置和方法。

    Bacterial delivery vehicles comprising tracer nucleic acid sequences
    97.
    发明授权
    Bacterial delivery vehicles comprising tracer nucleic acid sequences 有权

    公开(公告)号：US11421222B2

    公开(公告)日：2022-08-23

    申请号：US16905656

    申请日：2020-06-18

    申请人： Eligo Bioscience

    发明人： Igor Stzepourginski ,  Daniel Garry

    IPC分类号： C12N15/10 ,  C12N7/00 ,  C12Q1/70 ,  C40B20/08

    摘要： The present disclosure relates generally to genetically tagged bacterial delivery vehicles comprising unique tracer nucleic acid sequences (herein referred to as “tracers”) for use in detecting and/or quantitating the presence of two or more different said bacterial delivery vehicles within a mixture of vehicles. The present disclosure relates to methods wherein the bacterial delivery vehicles are detected through, for example, performance of multiple cycles of amplification using primers that bind to sequences within the unique tracer. Such methods can be advantageously used in quality control to detect and quantitate mixtures of bacterial delivery vehicles within a pharmaceutical composition.

    Method and apparatus for producing sequence verified DNA
    99.
    发明授权
    Method and apparatus for producing sequence verified DNA 有权

    公开(公告)号：US10822605B2

    公开(公告)日：2020-11-03

    申请号：US14268832

    申请日：2014-05-02

    申请人： GENSINTA, INC.

    发明人： Austen Heinz ,  Anselm Levskaya ,  John T. Mulligan

    IPC分类号： C12N15/10 ,  C40B20/08 ,  C40B50/14

    摘要： A method of retrieving a subset of polynucleotide molecules from a mixture of polynucleotide molecules includes receiving a mixture of nucleotide sequences comprising one or more polynucleotide molecules, synthesizing one or more identifier (ID) regions onto the one or more polynucleotide molecules, and sequencing members of the population of polynucleotide molecules to associate the sequence of one or more of the molecules (the “Polynucleotide Sequence”) with the sequence of the attached ID region (the “ID Sequence”). The method also includes generating a bead-bound library of one or more beads comprising subsets of identical polynucleotide molecules. Each bead is identified by the ID Sequence of the associated Polynucleotide Sequence. The method further includes sequencing the one or more ID regions of each bead to generate ID Sequence information for each bead, combining the Polynucleotide Sequence information, the one or more ID Sequences, and coordinates of each bead to identify the Polynucleotide Sequence on the bead, and retrieving the bead with its associated Polynucleotide Sequence from the flow cell based on the absolute coordinate position of the bead.