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107 条记录 (耗时 0.065 秒)

    Enzyme-mediated modification of fivrin for tissue engineering
    101.
    发明授权
    Enzyme-mediated modification of fivrin for tissue engineering 有权
    酶介导的组织工程纤维蛋白修饰

    公开(公告)号：US06607740B1

    公开(公告)日：2003-08-19

    申请号：US09695466

    申请日：2000-10-24

    申请人： Jeffrey A. Hubbell ,  Jason Schense

    发明人： Jeffrey A. Hubbell ,  Jason Schense

    IPC分类号： C12N910

    CPC分类号： A61L31/046 ,  A61F2310/00377 ,  A61L24/106 ,  A61L27/225 ,  A61L27/227 ,  C07K2319/00 ,  C12N5/0068 ,  C12N2533/56

    摘要： The invention provides fibrin-based, biocompatible materials useful in promoting cell growth, wound healing, and tissue regeneration. These materials are provided as part of several cell and tissue scaffolding structures that provide particular application for use in wound-healing and tissue regenerating. Methods for preparing these compositions and using them are also disclosed as part of the invention. A variety of peptides may be used in conjunction with the practice of the invention, in particular, the peptide IKVAV, and variants thereof. Generally, the compositions may be described as comprising a protein network (e.g., fibrin) and a peptide having an amino acid sequence that comprises a transglutaminase substrate domain (e.g., a factor XIIIa substrate domain) and a bioactive factor (e.g., a peptide or protein, such as a polypeptide growth factor), the peptide being covalently bound to the protein network. Other applications of the technology include their use on implantable devices (e.g., vascular graphs), tissue and cell scaffolding. Other applications include use in surgical adhesive or sealant, as well as in peripheral nerve regeneration and angiogenesis.

    摘要翻译： 本发明提供了用于促进细胞生长，伤口愈合和组织再生的基于纤维蛋白的生物相容性材料。 提供这些材料作为几种细胞和组织脚手架结构的一部分，其提供用于伤口愈合和组织再生的特定应用。 制备这些组合物并使用它们的方法也作为本发明的一部分公开。 多种肽可以与本发明的实践结合使用，特别是肽IKVAV及其变体。 通常，组合物可以被描述为包括蛋白质网络（例如，纤维蛋白）和具有包含转谷氨酰胺酶底物结构域（例如，因子XIIIa底物结构域）和生物活性因子（例如肽或其衍生物）的氨基酸序列的肽 蛋白质，例如多肽生长因子），肽共价结合蛋白质网络。 该技术的其他应用包括它们在可植入装置（例如血管图），组织和细胞支架上的使用。 其他应用包括用于手术粘合剂或密封剂，以及在周围神经再生和血管生成中。

    Enzyme-mediated modification of fibrin for tissue engineering: incorporation of proteins
    102.
    发明授权
    Enzyme-mediated modification of fibrin for tissue engineering: incorporation of proteins 有权
    酶介导的纤维蛋白修饰组织工程：掺入蛋白质

    公开(公告)号：US06468731B1

    公开(公告)日：2002-10-22

    申请号：US09675922

    申请日：2000-09-29

    申请人： Jeffrey A. Hubbell ,  Jason C. Schense ,  Shelly E. Sakiyama

    发明人： Jeffrey A. Hubbell ,  Jason C. Schense ,  Shelly E. Sakiyama

    IPC分类号： A01N102

    CPC分类号： C07K14/475

    摘要： Disclosed are materials that may be used in the design of improved devices and wound treatment platforms though covalent and/or non-covalent attachment of bioactive proteins. The proteins comprise any variety of cell growth and/or healing promoting proteins, such as growth factor. The incorporation of these whole proteins may be designed to provide controlled release thereof in a biological system through further use of enzyme degradation sites. Heparin-binding protein or fusion proteins synthesized to contain a heparin binding domain are two mechanisms that may be used in providing these properties to a matrix, such as a fibrinogen matrix. The proteins will be used to provide enhanced healing in various tissues including vasculature, skin, nerve, and liver. The materials disclosed will be used to enhance would healing and other generative processes by engineering the fibrin gel to contain appropriate proteins with specifically designed release and/or degradation characteristics.

    摘要翻译： 公开了可以用于设计改进的装置和伤口治疗平台的材料，尽管共价和/或非共价连接生物活性蛋白质。 蛋白质包括任何多种细胞生长和/或愈合促进蛋白质，例如生长因子。 可以将这些全蛋白的掺入设计成通过进一步使用酶降解位点来提供其在生物系统中的受控释放。 合成含有肝素结合结构域的肝素结合蛋白或融合蛋白是可用于向基质例如纤维蛋白原基质提供这些性质的两种机制。 蛋白质将用于在各种组织中提供增强的愈合，包括脉管系统，皮肤，神经和肝脏。 所公开的材料将用于通过将纤维蛋白凝胶工程化以含有具有专门设计的释放和/或降解特征的合适蛋白质来增强愈合和其它生成过程。

    Multifunctional organic polymers
    105.
    发明授权
    Multifunctional organic polymers 失效
    多功能有机聚合物

    公开(公告)号：US5627233A

    公开(公告)日：1997-05-06

    申请号：US465949

    申请日：1995-06-06

    申请人： Jeffrey A. Hubbell ,  Donald Elbert ,  Jennifer L. Hill-West ,  Paul D. Drumheller ,  Sanghamitra Chowdhury ,  Amarpreet Sawhney

    发明人： Jeffrey A. Hubbell ,  Donald Elbert ,  Jennifer L. Hill-West ,  Paul D. Drumheller ,  Sanghamitra Chowdhury ,  Amarpreet Sawhney

    IPC分类号： A61L27/00 ,  A61K9/50 ,  A61K47/48 ,  A61L31/06 ,  A61L33/00 ,  C08G63/91 ,  C08G73/00 ,  C08G81/00 ,  C08G81/02 ,  C08L77/00 ,  C08L79/00 ,  C08L101/00 ,  C09D187/00 ,  C08L1/00 ,  C08G69/48

    CPC分类号： C08G81/00 ,  A61K47/48238 ,  A61K47/48315 ,  A61K9/5031 ,  A61K9/5073 ,  A61L31/06

    摘要： Described herein is a multi-functional polymeric material for use in inhibiting adhesion and immune recognition between cells and cells, cells and tissues, and tissues and tissues. One component of the polymeric material adsorbs well to cells or tissue, and the other component of the polymeric material does not adsorb well to tissues. A water-soluble polymer that does not bear charge (polynonion) is used as the non-binding component, and a water soluble polymer that is positively charged at physiological pH (polycation) is used as the tissue binding component. When the bi-functional polymeric material contacts a tissue, the tissue-binding component binds and thus immobilizes the attached non-binding component, which will then extend generally away from the tissue surface and sterically block the attachment of other tissues. The method and compositions are useful in inhibiting formation of post-surgical adhesions, protecting damaged blood vessels from thrombosis and restenosis, and decreasing the extent of metastasis of attachment-dependent tumor cells.

    摘要翻译： 本文描述的是用于抑制细胞和细胞，细胞和组织以及组织和组织之间的粘附和免疫识别的多功能聚合物材料。 聚合物材料的一个组分很好地吸附到细胞或组织上，而聚合物材料的其它组分不能很好地吸附到组织上。 使用不带电荷的水溶性聚合物（多项式）作为非结合成分，使用在生理pH（聚阳离子）下带正电的水溶性聚合物作为组织结合成分。 当双官能聚合物材料接触组织时，组织结合组分结合并因此固定所连接的非结合组分，其然后将一般远离组织表面延伸，并空间阻断其它组织的附着。 该方法和组合物可用于抑制手术后粘连的形成，保护受损的血管免受血栓形成和再狭窄，并降低附着依赖性肿瘤细胞的转移程度。

    Methods for modifying cell contact with a surface
    106.
    发明授权
    Methods for modifying cell contact with a surface 失效
    修改细胞与表面接触的方法

    公开(公告)号：US5567440A

    公开(公告)日：1996-10-22

    申请号：US471390

    申请日：1995-06-06

    申请人： Jeffrey A. Hubbell ,  Donald Elbert ,  Jennifer L. Hill-West ,  Paul D. Drumbeller ,  Sanghamitra Chowdhury ,  Amarpreet Sawhney

    发明人： Jeffrey A. Hubbell ,  Donald Elbert ,  Jennifer L. Hill-West ,  Paul D. Drumbeller ,  Sanghamitra Chowdhury ,  Amarpreet Sawhney

    IPC分类号： A61L27/00 ,  A61K9/50 ,  A61K47/48 ,  A61L31/06 ,  A61L33/00 ,  C08G63/91 ,  C08G73/00 ,  C08G81/00 ,  C08G81/02 ,  C08L77/00 ,  C08L79/00 ,  C08L101/00 ,  C09D187/00 ,  A61K9/14

    CPC分类号： C08G81/00 ,  A61K47/48238 ,  A61K47/48315 ,  A61K9/5031 ,  A61K9/5073 ,  A61L31/06

    摘要： Described herein is a multi-functional polymeric material for use in inhibiting adhesion and immune recognition between cells and cells, cells and tissues, and tissues and tissues. One component of the polymeric material adsorbs well to cells or tissue, and the other component of the polymeric material does not adsorb well to tissues. A water-soluble polymer that does not bear charge (polynonion) is used as the non-binding component, and a water soluble polymer that is positively charged at physiological pH (polycation) is used as the tissue binding component. When the bi-functional polymeric material contacts a tissue, the tissue-binding component binds and thus immobilizes the attached non-binding component, which will then extend generally away from the tissue surface and sterically block the attachment of other tissues. The method and compositions are useful in inhibiting formation of post-surgical adhesions, protecting damaged blood vessels from thrombosis and restenosis, and decreasing the extent of metastasis of attachment-dependent tumor cells.

    摘要翻译： 本文描述的是用于抑制细胞和细胞，细胞和组织以及组织和组织之间的粘附和免疫识别的多功能聚合物材料。 聚合物材料的一个组分很好地吸附到细胞或组织上，而聚合物材料的其它组分不能很好地吸附到组织上。 使用不带电荷的水溶性聚合物（多项式）作为非结合成分，使用在生理pH（聚阳离子）下带正电的水溶性聚合物作为组织结合成分。 当双官能聚合物材料接触组织时，组织结合组分结合并因此固定所连接的非结合组分，其然后将一般远离组织表面延伸，并空间阻断其它组织的附着。 该方法和组合物可用于抑制手术后粘连的形成，保护受损的血管免受血栓形成和再狭窄，并降低附着依赖性肿瘤细胞的转移程度。