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103.发明申请Devices and Methods for Controlling Bubble Formation in Microfluidic Devices 审中-公开用于控制微流控装置中气泡形成的装置和方法公开(公告)号：US20070280856A1
公开(公告)日：2007-12-06
申请号：US11422057
申请日：2006-06-02
申请人： Umberto Ulmanella , Eric S. Nordman , Maengseok Song , Joon Mo Yang , Julie C. Lee , Nigel P. Beard , Min Yue , Carol Schembri , David Liu
发明人： Umberto Ulmanella , Eric S. Nordman , Maengseok Song , Joon Mo Yang , Julie C. Lee , Nigel P. Beard , Min Yue , Carol Schembri , David Liu
IPC分类号： B01L3/02
CPC分类号： B01L3/502746 , B01L3/502723 , B01L3/502784 , B01L2200/0642 , B01L2200/0673 , B01L2200/0684 , B01L2300/0816 , B01L2300/0864 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , B01L2400/086
摘要： A microfluidic device may include a sample distribution network including a plurality of sample chambers configured to be loaded with biological sample for biological testing of the biological sample while in the sample chambers, the biological sample having a meniscus that moves within the sample chambers during loading. The sample distribution network may further include a plurality of inlet channels, each inlet channel being in flow communication with and configured to flow biological sample to a respective sample chamber, and a plurality of outlet channels, each outlet channel being in flow communication and configured to flow biological sample from a respective sample chamber. At least some of the sample chambers may include a physical modification configured to control the movement of the meniscus so as to control bubble formation within the at least some sample chambers. At least some of the sample chambers may include a dried reagent positioned within the at least some sample chambers proximate the inlet channels in flow communication with the at least some sample chambers.
摘要翻译： 微流体装置可以包括样品分配网络，其包括多个样品室，其被配置为装载用于生物样品的生物样品的生物样品，而在样品室中，生物样品具有在加载期间在样品室内移动的弯液面。 样品分配网络还可以包括多个入口通道，每个入口通道与生物样品流动连通并配置成将生物样品流动到相应的样品室，以及多个出口通道，每个出口通道流动连通并且被配置为 从相应的样品室流动生物样品。 至少一些样品室可以包括物理修改，其被配置为控制弯液面的运动，以便控制至少一些样品室内的气泡形成。 至少一些样品室可以包括位于与至少一些样品室流动连通的入口通道附近的至少一些样品室内的干燥试剂。
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公开(公告)号：US20070264692A1
公开(公告)日：2007-11-15
申请号：US11688073
申请日：2007-03-19
申请人： David Liu , Allen Buskirk
发明人： David Liu , Allen Buskirk
IPC分类号： C12P21/06
CPC分类号： C07H21/04 , C07K14/35 , C07K14/395 , C07K2319/90 , C12N9/14 , C12N15/62
摘要： Ligand-dependent inteins allow for modulation of a protein's activity in vivo. Upon binding of the ligand to the ligand-dependent intein inserted into the protein of interest, the hybrid protein undergoes protein splicing removing the intein. The activity of the spliced protein is then restored. A 4-hydroxytamoxifen-dependent intein based on the M. tuberculosis RecA intein is prepared and demonstrated in a variety of exteins contexts. The invention provides a system for engineering other ligand-dependent inteins and using them, including the ligand-dependent inteins themselves, hybrid proteins with the inserted ligand-dependent inteins, polynucleotides encoding inteins and hybrid proteins, and engineered cells. Kits with the materials and reagents necessary for preparing and using ligand-dependent inteins are also included.
摘要翻译： 依赖于配体的内含肽允许在体内调节蛋白质的活性。 当配体与插入目的蛋白质中的配体依赖内含肽结合时，杂交蛋白经蛋白质剪接除去内含肽。 然后恢复剪接的蛋白质的活性。 基于结核分枝杆菌RecA内含肽的4-羟基他莫昔芬依赖性内含肽在各种exteins背景下制备和证实。 本发明提供了一种用于工程化其他配体依赖性内含肽并使用它们的系统，包括配体依赖性内含肽本身，与插入的配体依赖内含肽的杂交蛋白，编码内含肽和杂交蛋白的多核苷酸和工程细胞。 还包括使用制备和使用配体依赖内含肽所必需的材料和试剂的试剂盒。
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公开(公告)号：US20060197122A1
公开(公告)日：2006-09-07
申请号：US11382474
申请日：2006-05-09
申请人： Tsu-Jae King , David Liu
发明人： Tsu-Jae King , David Liu
IPC分类号： H01L29/76
CPC分类号： H01L29/42332 , H01L29/51 , H01L29/513 , H01L29/78 , H01L29/788 , H01L29/7881 , H01L29/792
摘要： A silicon based semiconductor device and method uses charge trapping to alter a density of carriers available in a channel of a field effect transistor (FET) for conduction. The charge trapping mechanism can be controlled by a source-drain bias voltages applied to the FET, so that the device can be turned off through a control mechanism separate from a gate voltage.
摘要翻译： 硅基半导体器件和方法使用电荷俘获来改变用于导电的场效应晶体管（FET）的通道中可用的载流子的密度。 可以通过施加到FET的源极 - 漏极偏置电压来控制电荷捕获机制，使得可以通过与栅极电压分离的控制机制来关闭器件。
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107.发明申请System and method for providing customized voice connection services via gatekeeper 失效通过网守提供定制语音连接服务的系统和方法公开(公告)号：US20050286711A1
公开(公告)日：2005-12-29
申请号：US10860590
申请日：2004-06-02
申请人： Fisher Lee , Ann Lee , David Liu
发明人： Fisher Lee , Ann Lee , David Liu
CPC分类号： H04L29/06027 , H04L12/5692 , H04L12/66 , H04L65/1009 , H04L65/1069 , H04L67/306 , H04L69/18 , H04M1/2535 , H04M3/42068 , H04M7/0057
摘要： A computer-implemented system is provided, including a network consisting of the Internet, PSTN, and CATV network. The network is connected to multiple users' client systems, and also to multiple service provider systems. Each user has a user profile stored in a User Preference Database, while each service provider has service parameters defining the type of its service stored in a Service Database, both of which are connected to the network. The system further includes a Gatekeeper Server, which establishes voice communication among the client systems and the service provider systems. In operation, upon receiving a user's request for service, the Gatekeeper Server identifies one or more service providers whose service parameters match the user's request for service and the user's profile. Upon the user's selection of one such service provider, the Gatekeeper server automatically selects and establishes a preferred mode of voice connection between the user and the selected service provider.
摘要翻译： 提供了一种计算机实现的系统，包括由因特网，PSTN和CATV网络组成的网络。 该网络连接到多个用户的客户端系统，并且还连接到多个服务提供商系统。 每个用户具有存储在用户偏好数据库中的用户简档，而每个服务提供商具有定义存储在服务数据库中的服务类型的服务参数，两者都连接到网络。 该系统还包括网守服务器，其在客户端系统和服务提供商系统之间建立语音通信。 在操作中，网守服务器在接收到用户的服务请求后，识别其服务参数与用户的服务请求和用户简档相匹配的一个或多个服务提供商。 当用户选择一个此类服务提供商时，网守服务器自动选择并建立用户与所选服务提供商之间的语音连接的优选模式。
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公开(公告)号：US20050281819A9
公开(公告)日：2005-12-22
申请号：US10744605
申请日：2003-12-23
申请人： David Liu , Zev Gartner , Matthew Kanan
发明人： David Liu , Zev Gartner , Matthew Kanan
IPC分类号： C12N15/09 , C07B61/00 , C08G85/00 , C12N15/10 , C12P1/00 , C12P19/34 , C12P21/00 , A61K39/395
CPC分类号： C12N15/1068 , C12P1/00 , C12P19/34 , C12P21/00
摘要： Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The present invention provides methods, compositions, and systems for synthesizing, selecting, amplifying, and evolving non-natural molecules based on nucleic acid templates. The sequence of a nucleic acid template is used to direct the synthesis of non-natural molecules such as unnatural polymers and small molecules. Using this method combinatorial libraries of these molecules can be prepared and screened. Upon selection of a molecule, its encoding nucleic acid template may be amplified and/or evolved to yield the same molecule or related molecules for re-screening. The inventive methods and compositions of the present invention allow for the amplification and evolution of non-natural molecules in a manner analogous to the amplification of natural biopolymer such as polynucleotides and protein.
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公开(公告)号：US20050248332A1
公开(公告)日：2005-11-10
申请号：US10923758
申请日：2004-08-23
申请人： David Liu
发明人： David Liu
CPC分类号： H02M1/10
摘要： A universal power converter comprises a body and a circuit module. The circuit module includes a power converter circuit; the power converter circuit being a power supply and charging circuit of a power supply; and a voltage adjusting circuit; the voltage adjusting circuit including a main controller; the main controller being installed with a plurality of output ports; each output port being serially connected to a respective transistor and a specific resistor value; each resistor value representing a specific output voltage which is feedback to the power converter circuit; the main controller serving for automatically opening or closing a selected voltage and for driving the signals from the data display screen and the actuating switch.
摘要翻译： 通用功率转换器包括主体和电路模块。 电路模块包括电源转换器电路; 电源转换器电路是电源的电源和充电电路; 和电压调节电路; 所述电压调整电路包括主控制器; 主控制器安装有多个输出端口; 每个输出端口串联连接到相应的晶体管和特定的电阻器值; 每个电阻值表示反馈到功率转换器电路的特定输出电压; 主控制器用于自动打开或关闭所选电压并用于驱动来自数据显示屏和致动开关的信号。
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公开(公告)号：US20050170376A1
公开(公告)日：2005-08-04
申请号：US10950367
申请日：2004-09-24
申请人： David Liu , Zev Gartner , Jeffrey Doyon , Christopher Calderone , Matthew Kanan , Xiaoyu Li , Thomas Snyder , Daniel Rosenbaum
发明人： David Liu , Zev Gartner , Jeffrey Doyon , Christopher Calderone , Matthew Kanan , Xiaoyu Li , Thomas Snyder , Daniel Rosenbaum
CPC分类号： C12Q1/68 , C12N15/1068
摘要： Nature evolves biological molecules such as proteins through iterated rounds of diversification, selection, and amplification. The power of Nature and the flexibility of organic synthesis are combined in nucleic acid-templated synthesis. The present invention provides a variety of template architectures for performing nucleic acid-templated synthesis, methods for increasing the selectivity of nucleic acid-templated reactions, methods for performing stereoselective nucleic acid-templated reactions, methods of selecting for reaction products resulting from nucleic acid-templated synthesis, and methods of identifying new chemical reactions based on nucleic acid-templated synthesis.
摘要翻译： 自然会通过迭代多样化，选择和扩增进化生物分子，如蛋白质。 自然的力量和有机合成的灵活性结合在核酸模板合成中。 本发明提供用于进行核酸模板合成的多种模板结构，用于增加核酸模板化反应的选择性的方法，用于进行立体选择性核酸模板化反应的方法，选择由核酸 - 模板合成，以及基于核酸模板合成鉴定新化学反应的方法。
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