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    Method of detecting genetic polymorphism
    101.
    发明授权
    Method of detecting genetic polymorphism 有权
    检测遗传多态性的方法

    公开(公告)号：US07888024B2

    公开(公告)日：2011-02-15

    申请号：US12058318

    申请日：2008-03-28

    申请人： Naoya Hosono ,  Mitsuaki Kubo ,  Yusuke Nakamura

    发明人： Naoya Hosono ,  Mitsuaki Kubo ,  Yusuke Nakamura

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02

    CPC分类号： C12Q1/6827 ,  C12Q2561/109 ,  C12Q2537/157

    摘要： The present invention provides a novel polymorphism detecting method suitable for the detection and identification of copy number variation.Provided is a method of determining the genotype of a subject in a genomic region comprising an SNP site, comprising a step for performing typing of the SNP site by the invader assay with a DNA-containing sample comprising the genomic region from the subject as the template, wherein fluorescence is measured on a real time basis. The copy number ratio of both alleles is determined using the fluorescence intensity ratio of each allele at a time before saturation of fluorescence intensity. Preferably, the present method further comprises a step for amplifying the genomic region comprising an SNP site prior to the invader step. In this step of amplification, a plurality of regions comprising a plurality of SNP sites can be simultaneously amplified. Furthermore, the present method enables the determination of the copy number of each allele when combined with quantitative PCR.

    摘要翻译： 本发明提供了一种适用于检测和鉴定拷贝数变异的新型多态性检测方法。 提供了确定包含SNP位点的基因组区域中受试者的基因型的方法，其包括通过使用含有来自受试者的基因组区域作为模板的含有DNA的样品通过侵入测定进行SNP位点的分型的步骤 其中荧光是实时测量的。 使用荧光强度饱和之前的每个等位基因的荧光强度比来确定两个等位基因的拷贝数比。 优选地，本方法还包括在入侵者步骤之前扩增包含SNP位点的基因组区域的步骤。 在该扩增步骤中，可以同时扩增包含多个SNP位点的多个区域。 此外，当与定量PCR组合时，本方法能够确定每个等位基因的拷贝数。

    Method of Dispensing in Reaction Vessel and Reaction Vessel Processing Apparatus
    103.
    发明申请
    Method of Dispensing in Reaction Vessel and Reaction Vessel Processing Apparatus 审中-公开
    反应容器和反应容器加工装置中的分配方法

    公开(公告)号：US20100196209A1

    公开(公告)日：2010-08-05

    申请号：US11887507

    申请日：2006-03-30

    申请人： Nobuhiro Hanafusa ,  Koretsugu Ogata ,  Ryuh Konoshita ,  Yusuke Nakamura ,  Yozo Ohnishi

    发明人： Nobuhiro Hanafusa ,  Koretsugu Ogata ,  Ryuh Konoshita ,  Yusuke Nakamura ,  Yozo Ohnishi

    IPC分类号： G01N35/10

    CPC分类号： B01L3/0262 ,  B01L2400/022 ,  B01L2400/024 ,  G01N35/1016 ,  G01N35/1095

    摘要： It is intended to facilitate dispensing of a minute amount of a nonvolatile liquid. In a preferred embodiment, in dispensing of mineral oil (40) onto a reaction solution (170) that is previously dispensed to a probe arrangement part (18), a liquid droplet (40a) of the mineral oil (40) is formed on a tip end of a tip (70) of the nozzle, and the liquid droplet (40a) is transferred into the reaction well while it is in contact with the inner wall face of the reaction well or the surface of the reaction solution (170) previously dispensed to the reaction well.

    摘要翻译： 旨在便于分配微量的非挥发性液体。 在优选的实施方案中，在将矿物油（40）分配到预先分配到探针装置部分（18）的反应溶液（170）上时，矿物油（40）的液滴（40a）形成在 喷嘴的尖端（70）的尖端，并且液滴（40a）在与反应井的内壁面或反应溶液（170）的表面接触的同时被转移到反应槽中 分配到反应良好。

    Method of judging inflammatory disease
    104.
    发明授权
    Method of judging inflammatory disease 失效
    炎症性疾病的诊断方法

    公开(公告)号：US07754422B2

    公开(公告)日：2010-07-13

    申请号：US10523723

    申请日：2003-08-08

    申请人： Toshihiro Tanaka ,  Yozo Ohnishi ,  Koichi Ozaki ,  Aritoshi Iida ,  Yusuke Nakamura ,  Masatsugu Hori

    发明人： Toshihiro Tanaka ,  Yozo Ohnishi ,  Koichi Ozaki ,  Aritoshi Iida ,  Yusuke Nakamura ,  Masatsugu Hori

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/172

    摘要： The object of the present invention is to determine inflammatory diseases such as myocardial infarction by identifying single nucleotide polymorphisms (SNPs) associated with myocardial infarction and utilizing these SNPs. The present invention provides a method for determining an inflammatory disease, which comprises detecting at least one gene polymorphism existing in at least one gene selected from the group consisting of a lymphotoxin-α (LT-α) gene, an I Kappa B-like (IKBL) gene, and a BAT1 gene; an oligonucleotide used in said method; a kit for diagnosing an inflammatory disease which comprises said oligonucleotide; and use thereof. The present invention further provides a method for treating an inflammatory disease; and a method for screening for a therapeutic agent for an inflammatory disease.

    摘要翻译： 本发明的目的是通过鉴定与心肌梗塞相关的单核苷酸多态性（SNP）和利用这些SNP来确定诸如心肌梗死的炎性疾病。 本发明提供了一种测定炎性疾病的方法，该方法包括检测至少一种存在于选自淋巴毒素-α（LT-α）基因，IκB样（LT-α） IKBL）基因和BAT1基因; 所述方法中使用的寡核苷酸; 用于诊断包含所述寡核苷酸的炎性疾病的试剂盒; 及其用途。 本发明还提供一种治疗炎性疾病的方法; 以及筛选炎性疾病治疗剂的方法。

    Liquid crystal display device operated by remote control
    106.
    发明授权
    Liquid crystal display device operated by remote control 有权
    液晶显示装置由遥控器操作

    公开(公告)号：US07679592B2

    公开(公告)日：2010-03-16

    申请号：US11336866

    申请日：2006-01-23

    申请人： Yusuke Nakamura

    发明人： Yusuke Nakamura

    IPC分类号： G09G3/36

    CPC分类号： G09G3/3655 ,  G09G2320/0247 ,  H04N21/4221 ,  H04N2005/4428

    摘要： An LCD device operated by a remote controller comprises: an LCD unit having a display electrode supplied with voltage based on a video signal and a counter-electrode supplied with counter-electrode voltage; a remote control receiver for receiving a flicker adjustment control signal from the remote controller; a microcomputer for outputting a flicker voltage control signal based on the flicker adjustment control signal; a counter-electrode voltage control unit for generating a flicker adjustment voltage whose value varies according to the flicker voltage control signal, and outputting a DC counter-electrode voltage based on the flicker adjustment voltage; and a DC-AC converter for converting the DC counter-electrode voltage to AC voltage to be applied to the counter-electrode. An operator (human) can make flicker adjustment at a position on the front side of, and if necessary distanced from, the LCD device where the operator can directly and easily view an image on the LCD.

    摘要翻译： 由遥控器操作的LCD装置包括：LCD单元，具有基于视频信号提供电压的显示电极和提供有对电极电压的对电极; 遥控接收器，用于从遥控器接收闪烁调节控制信号; 微计算机，用于基于闪烁调整控制信号输出闪烁电压控制信号; 对置电极电压控制单元，用于产生闪烁调整电压，其值根据闪烁电压控制信号而变化，并基于闪烁调整电压输出DC对置电极电压; 以及用于将DC对置电极电压转换为施加到对电极的AC电压的DC-AC转换器。 操作人员（人类）可以在液晶显示器的正面，如果需要远离LCD设备的位置进行闪烁调节，操作员可以直接轻松地在LCD上观看图像。

    METHOD OF DIAGNOSING SMALL CELL LUNG CANCER
    109.
    发明申请
    METHOD OF DIAGNOSING SMALL CELL LUNG CANCER 审中-公开
    诊断小细胞肺癌的方法

    公开(公告)号：US20090317392A1

    公开(公告)日：2009-12-24

    申请号：US11913273

    申请日：2006-07-26

    申请人： Yusuke Nakamura ,  Yataro Daigo ,  Shuichi Nakatsuru

    发明人： Yusuke Nakamura ,  Yataro Daigo ,  Shuichi Nakatsuru

    IPC分类号： A61K39/395 ,  A61K39/00 ,  C12Q1/68 ,  G01N33/53 ,  C12N15/63 ,  C40B30/04 ,  A61K31/7088 ,  C07H21/02

    CPC分类号： C12N15/1135 ,  C12N2310/14 ,  C12Q1/6886 ,  C12Q2600/136

    摘要： Objective methods for detecting and diagnosing small cell lung cancer (SCLC) are described herein. In one embodiment, the diagnostic method involves determining the expression level of an SCLC-associated gene that discriminates between SCLC cells and normal cells. In another embodiment, the diagnostic method involves determining the expression level of an SCLC-associated gene that distinguishes two major histological types of lung cancer, non-small cell lung cancer (NSCLC) and SCLC. Finally, the present invention provides methods of screening for therapeutic agents useful in the treatment of small cell lung cancer, methods of treating small cell lung cancer and method for vaccinating a subject against small cell lung cancer. Furthermore, the present invention provides chemotherapy resistant lung cancer- or SCLC-associated genes as diagnostic markers and/or molecular targets for therapeutic agent for these cancers. These genes are up-regulated in chemoresistant lung cancer or SCLC. Accordingly, chemoresistant lung cancer or SCLC can be predicted using expression level of the genes as diagnostic markers. As the result, any adverse effects caused by ineffective chemotherapy can be avoided, and more suitable and effective therapeutic strategy can be selected.

    摘要翻译： 本文描述了用于检测和诊断小细胞肺癌（SCLC）的客观方法。 在一个实施方案中，所述诊断方法涉及确定区分SCLC细胞和正常细胞的SCLC相关基因的表达水平。 在另一个实施方案中，诊断方法包括确定区分肺癌，非小细胞肺癌（NSCLC）和SCLC的两种主要组织学类型的SCLC相关基因的表达水平。 最后，本发明提供了筛选用于治疗小细胞肺癌的治疗剂，治疗小细胞肺癌的方法和接种针对小细胞肺癌的受试者的方法。 此外，本发明提供抗化学疗法的肺癌或SCLC相关基因作为用于这些癌症的治疗剂的诊断标记和/或分子靶标。 这些基因在化疗耐药性肺癌或SCLC中上调。 因此，可以使用基因的表达水平作为诊断标记来预测化学耐药性肺癌或SCLC。 结果，可以避免无效化疗引起的任何不良反应，可以选择更合适有效的治疗策略。

    METHOD OF DIAGNOSING BLADDER CANCER
    110.
    发明申请
    METHOD OF DIAGNOSING BLADDER CANCER 失效
    诊断乳腺癌的方法

    公开(公告)号：US20090175844A1

    公开(公告)日：2009-07-09

    申请号：US11815850

    申请日：2006-02-09

    申请人： Yusuke Nakamura ,  Toyomasa Katagiri ,  Shuichi Nakatsuru

    发明人： Yusuke Nakamura ,  Toyomasa Katagiri ,  Shuichi Nakatsuru

    IPC分类号： A61K39/395 ,  C12Q1/68 ,  C40B30/00 ,  C07K14/00 ,  C07H21/02 ,  C12N15/63 ,  C12N5/10 ,  C12P21/02 ,  C07K16/00 ,  A61K31/7105 ,  A61K38/16 ,  A61P35/00

    CPC分类号： C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N33/57407 ,  G01N2500/00

    摘要： Objective methods for detecting and diagnosing bladder cancer (BLC) are described herein. In one embodiment, the diagnostic method involves determining the expression level of a BLC-associated gene that discriminates between BLC cells and normal cells. The present invention further provides means for predicting and preventing bladder cancer metastasis using BLC-associated genes having unique altered expression patterns in bladder cancer cells with lymph-node metastasis. Finally, the present invention provides methods of screening for therapeutic agents useful in the treatment of bladder cancer, methods of treating bladder cancer and method for vaccinating a subject against bladder cancer. In particular, the present application provides novel human genes C2093, B5860Ns and C6055s whose expression is markedly elevated in bladder cancers. The genes and polypeptides encoded by the genes can be used, for example, in the diagnosis of bladder cancers, as target molecules for developing drugs against the disease, and for attenuating cell growth of bladder cancer.

    摘要翻译： 本文描述了膀胱癌（BLC）检测和诊断的客观方法。 在一个实施方案中，诊断方法涉及确定鉴别BLC细胞和正常细胞的BLC相关基因的表达水平。 本发明还提供了使用具有淋巴结转移的膀胱癌细胞中具有独特改变的表达模式的BLC相关基因来预测和预防膀胱癌转移的手段。 最后，本发明提供筛选可用于治疗膀胱癌的治疗剂，治疗膀胱癌的方法和接种疫苗接种膀胱癌的方法。 特别地，本申请提供了新的人基因C2093，B5860Ns和C6055，其表达在膀胱癌中显着升高。 由该基因编码的基因和多肽可用于例如膀胱癌的诊断，作为用于开发针对该疾病的药物的靶分子，以及用于减轻膀胱癌的细胞生长。