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203 条记录 (耗时 0.017 秒)

    Method for producing microbial cells and use thereof to produce oxidation products
    105.
    发明授权
    Method for producing microbial cells and use thereof to produce oxidation products 失效
    生产微生物细胞的方法及其生产氧化产物的用途

    公开(公告)号：US4375515A

    公开(公告)日：1983-03-01

    申请号：US215248

    申请日：1980-12-11

    申请人： Ramesh N. Patel ,  Ching-Tsang Hou ,  Allen I. Laskin

    发明人： Ramesh N. Patel ,  Ching-Tsang Hou ,  Allen I. Laskin

    IPC分类号： C07D301/02 ,  C07D303/04 ,  C12N1/16 ,  C12N1/20 ,  C12N1/30 ,  C12N1/32 ,  C12N9/02 ,  C12N9/04 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/26 ,  C12P7/28 ,  C12P17/02

    CPC分类号： C12N9/0073 ,  C07D301/02 ,  C07D303/04 ,  C12N1/16 ,  C12N1/20 ,  C12N1/30 ,  C12N1/32 ,  C12N9/0006 ,  C12P17/02 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/26 ,  C12P7/28 ,  C12Y114/13025 ,  Y02E50/17 ,  Y10S435/822 ,  Y10S435/858

    摘要： Disclosed are newly discovered and isolated methylotrophic microorganism strains and their natural and/or artificial mutants which grow well under aerobic conditions in a culture medium in the presence of methane as the major carbon and energy source. The methane-grown microbial cells possess a high content of protein and can be utilized as such as feedstuffs. The methane-grown microbial cells or enzyme preparations thereof are also useful in converting C.sub.1 -C.sub.6 alkanes to alcohols, particularly methane to methanol, C.sub.3 -C.sub.6 alkanes to the corresponding C.sub.3 -C.sub.6 sec. alcohols and methyl ketones, C.sub.3 -C.sub.6 sec. alcohols to the corresponding methyl ketones, cyclic hydrocarbons to cyclic hydrocarbyl alcohols (e.g., cyclohexane to cyclohexanol) C.sub.2 -C.sub.4 alkenes selected from the group consisting of ethylene, propylene, butene-1 and butadiene to 1,2-epoxides, styrene to styrene oxide, and converting other oxidizable substrates to oxidized products. The newly discovered and isolated methylotrophic microorganism strains may be aerobically grown on a plurality of methyl-radical donating carbon-containing compounds, such as methanol, methylamine, methyl formate, methyl carbonate, dimethyl ether, etc., to produce microbial cells or enzyme preparations capable of aerobically converting C.sub.3 -C.sub.6 linear secondary alcohols to the corresponding methyl ketones.

    摘要翻译： 公开了新发现和分离的甲基营养性微生物菌株及其天然和/或人工突变体，其在甲烷作为主要碳源和能源的存在下，在有氧条件下在培养基中生长良好。 甲烷生长的微生物细胞具有高含量的蛋白质，可以像饲料一样使用。 甲烷生长的微生物细胞或其酶制剂也可用于将C1-C6烷烃转化为醇，特别是甲烷转化为甲醇，C3-C6烷烃转化为相应的C3-C6秒。 醇和甲基酮，C3-C6秒。 醇与相应的甲基酮，环状烃与环状烃类醇（例如环己烷至环己醇）选自乙烯，丙烯，丁烯-1和丁二烯至1,2-环氧化物的C2-C4烯烃，苯乙烯至苯乙烯氧化物 ，并将其它可氧化底物转化为氧化产物。 新发现和分离的甲基营养微生物菌株可以在多个含甲基供体的含碳化合物如甲醇，甲胺，甲酸甲酯，甲基碳酸酯，二甲醚等上进行有氧生长，以产生微生物细胞或酶制剂 能够有用地将C3-C6直链仲醇转化为相应的甲基酮。

    Production of protein with reduced nucleic acid
    106.
    发明授权
    Production of protein with reduced nucleic acid 失效
    用减少的核酸生产蛋白质

    公开(公告)号：US4341802A

    公开(公告)日：1982-07-27

    申请号：US200367

    申请日：1980-10-24

    申请人： Thomas R. Hopkins

    发明人： Thomas R. Hopkins

    IPC分类号： A21D2/26 ,  A23J1/00 ,  C12N1/08 ,  C12N1/32 ,  C12P21/00 ,  A23L1/28

    CPC分类号： A21D2/267 ,  A23J1/008 ,  C12N1/08 ,  C12N1/32 ,  Y10S435/804 ,  Y10S530/82 ,  Y10S530/821 ,  Y10S530/825

    摘要： Microorganisms with low nucleic acid content are produced by acid treating of the microorganism. One embodiment relates to the extraction of so treated microorganisms after their separation from the acid treating fluid, using a strong base for the extraction. Another embodiment relates to the neutralization of the acid treating fluid containing nucleic acids prior to the introduction of this fluid into the fermentation zone, the anion of the acid and the metal cation used in the neutralization step forming minerals desirable for the growth medium in the fermentation step.

    摘要翻译： 具有低核酸含量的微生物通过对微生物进行酸处理而产生。 一个实施方案涉及如此处理的微生物在与酸处理液分离后，使用强碱进行萃取。 另一个实施方案涉及在将该流体引入发酵区之前，含有酸的酸处理液的中和，在中和步骤中使用的酸阴离子和金属阳离子形成发酵生长培养基所需的矿物质 步。

    Biopolymer production process
    109.
    发明授权
    Biopolymer production process 失效
    生物聚合物生产工艺

    公开(公告)号：US4006058A

    公开(公告)日：1977-02-01

    申请号：US634986

    申请日：1975-11-24

    申请人： Joseph George Savins

    发明人： Joseph George Savins

    IPC分类号： C12N1/32 ,  C12P19/04 ,  C12D13/04

    CPC分类号： C12N1/32 ,  C12P19/04 ,  Y10S435/858

    摘要： An improved process for the production of a heteropolysaccharide by fermentation of a methanol containing culture medium with a microorganism of Methylomonas mucosa NRRL B-5696 wherein an alkali metal glycerophosphate is incorporated into the culture medium to provide a source of assimilable phosphate. An iron chelating agent selected from the group consisting of the alkali metal and the ferric/alkali metal salts of ethylenediaminetetraacetic acid may be added to the culture medium and N-tris-(hydroxymethyl)methyl glycine may be employed as a buffering agent. The heteropolysaccharide thus produced may be employed in the in vivo form to provide a viscous aqueous solution injected into a subterranean oil reservoir for mobility control purposes.

    摘要翻译： 通过用含有Methylomonas粘膜微生物NRRL B-56​​96的含甲醇培养基的发酵制备杂多糖的改进方法，其中将碱金属甘油磷酸酯引入培养基中以提供可同化磷酸盐的来源。 可以向培养基中加入选自碱金属和乙二胺四乙酸的铁/碱金属盐的铁螯合剂，可以使用N-三（羟甲基）甲基甘氨酸作为缓冲剂。 由此产生的杂多糖可以以体内形式使用，以提供注入到地下油储存器中用于移动控制目的的粘性水溶液。