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    Cell Settler Apparatus Systems and Methods for Perfusion Processes
    112.
    发明申请
    Cell Settler Apparatus Systems and Methods for Perfusion Processes 有权

    公开(公告)号：US20220145230A1

    公开(公告)日：2022-05-12

    申请号：US17609351

    申请日：2020-05-13

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Stephan KAISER

    IPC: C12M1/00 ,  C12M1/26 ,  C12M1/04 ,  C12M1/34

    Abstract: The present set of embodiments relate to a system, method, and apparatus for separating cells from a suspension using a gravity driven cell separation device. The apparatus may include a chamber that is separated into a gas compartment and a liquid compartment allowing for transfer of gas through a membrane into the liquid compartment where the cell suspension flows during operation. The embodiments are designed to maximize the gravitational separation effect on the suspension while mitigating the loss of surface area through which gas transfer can occur through a membrane to provide nutrients to cells in the suspension by tilting the cell separation device on two or more axes.

    SYSTEMS AND METHODS FOR RUN-TIME SEQUENCING RUN QUALITY MONITORING
    117.
    发明申请
    SYSTEMS AND METHODS FOR RUN-TIME SEQUENCING RUN QUALITY MONITORING 有权

    公开(公告)号：US20220072681A1

    公开(公告)日：2022-03-10

    申请号：US17478096

    申请日：2021-09-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Yerramalli Subramaniam ,  Puneet Suri ,  Sylvia Chang ,  Chengyong Yang

    IPC: B24B41/06 ,  G16B30/00 ,  G05B11/01 ,  B24B5/04

    Abstract: Systems and methods of providing run-time quality control and monitoring of a single or multiple sequencing runs are provided herein. In some embodiments, the run-time system includes or is in communication with a processor capable of determining various types of run-time information relating to the quality, progress, etc. of various sequencing runs. In some embodiments, the system can also be in communication with a user interface, for example, a GUI, capable of representing and communicating various types of information to a user regarding the quality of the individual or multiple runs, the functioning of the instrument, an error event, etc. Additionally, the system can capable of receiving actionable information from a user via the GUI thereby allowing the user to terminate or repeat various sequencing steps in a particular run, terminate a entire run, terminate all runs, allow a run to proceed, etc.

    Device, System and Method for Fluid Delivery for Sequencing
    118.
    发明申请
    Device, System and Method for Fluid Delivery for Sequencing 有权

    公开(公告)号：US20220065890A1

    公开(公告)日：2022-03-03

    申请号：US17524840

    申请日：2021-11-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Albert L. Carrillo ,  John Donohue

    IPC: G01N35/10 ,  G01N35/00 ,  B01L3/00

    Abstract: Various embodiments of a fluidic system of the present teachings are configured to execute a sequence of fluidic operations over the course of a next generation sequencing analysis for the sequential delivery of various solutions used over the course of analysis to a multilane sensor device. Exemplary fluidic operations include washing, priming and nucleotide reagent delivery through a fluidic multiplexer block that is configured to provide independent fluid distribution to each lane of a multilane sensor device used for detection during an analysis. Accordingly, any number or combination of lanes can be used during an analysis, so that during an analysis one lane in any position can be used singly during a run, all four lanes can be used simultaneously during a run, or any combination of lanes can be used simultaneously during a run.

    Flat-Field Imaging System and Methods of Use
    119.
    发明申请
    Flat-Field Imaging System and Methods of Use 有权

    公开(公告)号：US20220065767A1

    公开(公告)日：2022-03-03

    申请号：US17525477

    申请日：2021-11-12

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Mark ANDERSEN ,  Michael PALLAS ,  Haopeng Wang

    IPC: G01N15/04 ,  C12Q1/6816 ,  C12Q1/686

    Abstract: A method of aligning a plurality of targets is provided. The method includes generating a plurality of targets. A third phase includes the plurality of targets. The method further includes combining a first phase, a second phase, and the third phase in a volume. The first phase, the second phase, and the third phase are substantially immiscible, and the third phase is in fluid communication with the first phase and the second phase, and the first phase, the second phase, and the third phase are operable to be in a configuration of the third phase between the first phase and the second phase in the volume.

    SYSTEM AND METHOD INCORPORATING SOLID BUFFER
    120.
    发明申请
    SYSTEM AND METHOD INCORPORATING SOLID BUFFER 有权

    公开(公告)号：US20220057413A1

    公开(公告)日：2022-02-24

    申请号：US17465303

    申请日：2021-09-02

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： James A. BALL ,  Jonathan SCHULTZ

    IPC: G01N33/84 ,  C12Q1/6869 ,  B01J19/00

    Abstract: A buffered suspension includes a surfactant and a solid buffer particulate having a point of zero charge at least 1.2 pH units different that the pH of the buffered suspension. The buffered suspension can be prepared by mixing a stock solution with the solid buffer particulate and titrating. A method of preforming a pH sensitive process includes drawing the buffered suspension from a reservoir, filtering the solid buffer particulate from the buffered suspension, and applying the filtered solution to a sensor.

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