摘要:
Protein crystallization screening and optimization droplet actuators, systems and methods are provided. According to one embodiment, a screening droplet actuator is provided and includes: (a) a port for introduction of one or more crystallization reagents and/or one or more protein solutions; and (b) a substrate including: (i) an array of two or more mixing wells; and (ii) electric field mediated microfluidics for moving droplets comprising the crystallization reagents and protein solutions into the mixing wells. Optimization droplet actuators, systems including screening droplet actuators, methods of screening protein crystallization conditions, and methods of testing conditions for growing a crystal are also provided.
摘要:
The present invention relates to droplet-based affinity assays. According to one embodiment, a method of detecting a target analyte in a sample is provided, wherein the method includes: (a) executing droplet operations to combine affinity-based assay reagents on a droplet microactuator with a sample potentially comprising the target analyte to generate a signal indicative of the presence, absence and/or quantity of analyte; and (b) detecting the signal, wherein the signal corresponds to the presence, absence and/or quantity of the analyte in the sample.
摘要:
Methods and Systems are provided for a distributed Provider Edge (PE). A single Virtual Routing and Forwarding device (VRF) is associated with a single customer site. The VRF includes a single routing table (RIB) and a single forwarding table (FIB). The VRF also includes a plurality of Virtual Private Network (VPN) Protocol Instance Modules (VRP), where each VRP is associated with a different VPN from the customer site. Each VRP accesses the RIB directly and the FIB indirectly to acquiring addressing/routing information for a received data packet. Moreover, each VRP uses a data plane of the VRP to communicate the data packets to a PE backbone device. In turn, the PE backbone device uses the data plane to communicate with each of the VRPs, and the PE backbone device communicates with one or more tunnels.
摘要:
Methods, systems and computer program products for controlling scheduling in an ATM node in a network are provided which aggregate connection information for connections to the ATM node to provide a set of aggregate connection information for the ATM node. The set of aggregate connection information is distributed to other ATM nodes in the network and may be used in accepting connections to the ATM network based on the aggregate connection information. Scheduling may be provided by associating a timestamp value in each cell associated with an accepted connection as it is received by the ATM node. Received cells are grouped into a plurality of cell groups in timestamp order based upon characteristics of the cells and cells are selected from each group so as to transmit the cells in earliest deadline first order.