公开(公告)号：US20040002114A1

公开(公告)日：2004-01-01

申请号：US10328916

申请日：2003-01-06

申请人： METABOLEX, INC.

发明人： Francine M. Gregoire ,  Jeffrey D. Johnson ,  John E. Blume

IPC分类号： G01N033/53 ,  A61K038/17 ,  C07K014/72 ,  C12P021/02 ,  C12N005/06 ,  C07H021/04

CPC分类号： C07K14/705

摘要： The invention provides isolated nucleic acid and amino acid sequences of human and murine islet cell G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, methods of screening for modulators of islet cell G-protein coupled receptors and methods of treating mammals with modulators of islet cell G-protein coupled receptor activity.

摘要翻译： 本发明提供分离的人和鼠胰岛细胞G蛋白偶联受体的核酸和氨基酸序列，对这种受体的抗体，检测这种核酸和受体的方法，筛选胰岛细胞G蛋白偶联受体的调节剂的方法和 用胰岛细胞G蛋白偶联受体活性调节剂治疗哺乳动物的方法。

公开(公告)号：US6020200A

公开(公告)日：2000-02-01

申请号：US609164

申请日：1996-03-01

申请人： Karl C. Enevold

发明人： Karl C. Enevold

IPC分类号： C12N11/00 ,  A61K9/16 ,  A61K9/50 ,  A61K35/39 ,  A61K47/30 ,  A61P3/08 ,  C12N1/04 ,  C12N5/00 ,  C12N5/06

CPC分类号： B82Y30/00 ,  A61K9/1652 ,  A61K9/5052 ,  B82Y5/00 ,  C12N5/0012 ,  C12N2533/54 ,  C12N2533/74 ,  Y10S514/866 ,  Y10S514/965 ,  Y10T428/2984 ,  Y10T428/2989

摘要： The present invention generally relates to novel encapsulation compositions and methods. In particular, the invention relates to stabilized microcapsule compositions which comprise a layer of a crosslinked, mixed functionality, polymer matrix, and methods for their preparation. The encapsulated compositions may comprise the crosslinked polymer matrix layer as an inner layer, an outer layer, or an intermediate layer of an overall encapsulated composition. The compositions will generally also comprise a biological material, e.g., cells, proteins, and the like, encapsulated within the composition. The compositions and methods of the invention are useful in a variety of applications, including cell culturing and transplant therapy.

摘要翻译： 本发明一般涉及新颖的包封组合物和方法。 特别地，本发明涉及包含交联的混合官能团层，聚合物基质层及其制备方法的稳定的微胶囊组合物。 包封的组合物可以包含作为整体封装组合物的内层，外层或中间层的交联聚合物基质层。 组合物通常还包含封装在组合物内的生物材料，例如细胞，蛋白质等。 本发明的组合物和方法可用于多种应用，包括细胞培养和移植治疗。

公开(公告)号：US5693514A

公开(公告)日：1997-12-02

申请号：US300053

申请日：1994-09-02

申请人： Randel E. Dorian ,  Kent C. Cochrum

发明人： Randel E. Dorian ,  Kent C. Cochrum

IPC分类号： A01N1/02 ,  A61K9/16 ,  A61K9/50 ,  A61K35/12 ,  A61L27/20 ,  A61L27/34 ,  A61L27/36 ,  A61L27/38 ,  B01J2/00 ,  C12N5/00 ,  C12N5/071 ,  C12N11/04 ,  C12N11/10 ,  C12N11/12 ,  C12N11/08

CPC分类号： A01N1/0205 ,  A01N1/02 ,  A01N1/0231 ,  A61K9/1652 ,  A61K9/5031 ,  A61K9/5073 ,  A61K9/5089 ,  A61L27/20 ,  A61L27/34 ,  A61L27/36 ,  A61L27/38 ,  B01J2/003 ,  C12N11/04 ,  C12N5/0012 ,  C12N5/0677 ,  A61K2035/128 ,  C12N2500/12 ,  C12N2533/74 ,  Y10S623/92

摘要： A transplant with a core of a viable, physiologically active, cell(s) and a non-fibrogenic coating of alkaline earth metal alginate having a high mannuronate to guluronate molar ratio and free from fibrogenic amounts of fucose, sulfate, phloroglucinol and protein moieties. The coating has a permeability sufficiently low and a thickness sufficiently large to protect the tissue cells from host immunological agents after transplantation, the coating also being sufficiently permeable and thin to permit the diffusion of cell sufficient nutrients and cell products through the coating required for cell viability. The alginate coating can be reacted with polylysine to form a polylysine-alginate complex on the outer surface thereof. The complex can then be reacted with polyaspartic acid to provide a physiologically acceptable negative surface charge.

摘要翻译： 具有活性的，生理活性的细胞和具有高甘露糖酸盐至古洛糖醛酸盐摩尔比并且不含纤维含量岩藻糖，硫酸盐，间苯三酚和蛋白质部分的碱土金属藻酸盐的核心的移植物。 涂层具有足够低的渗透性和足够大的厚度以在移植后保护组织细胞免受宿主免疫剂的侵害，涂层也具有足够的渗透性和薄度，以允许细胞扩散足够的营养物和细胞产物通过细胞活力所需的涂层 。 藻酸盐涂层可与聚赖氨酸反应，以在其外表面上形成聚赖氨酸 - 藻酸盐复合物。 然后可以将复合物与聚天冬氨酸反应以提供生理上可接受的负表面电荷。