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公开(公告)号：US10106850B2
公开(公告)日：2018-10-23
申请号：US15683252
申请日：2017-08-22
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia Van Eijk , Anker Preben Sørensen , Marco Gerardus Maria Van Schriek
IPC: C12Q1/6874 , C12Q1/6827 , C12Q1/6869 , C12Q1/6855 , C12Q1/6883 , C12Q1/6876
Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
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公开(公告)号：US20180247017A1
公开(公告)日：2018-08-30
申请号：US15961512
申请日：2018-04-24
Applicant: Keygene N.V.
IPC: G06F19/22
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.
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公开(公告)号：US20180216122A1
公开(公告)日：2018-08-02
申请号：US15757020
申请日：2016-09-05
Applicant: Keygene N.V.
Inventor： Peter Johannes VAN DIJK , Diana RIGOLA , Marinus Willem PRINS , Adrianus Johannes VAN TUNEN
IPC: C12N15/82 , C12Q1/68 , C12N15/113 , C12N15/62
CPC classification number: C12N15/8201 , C12N15/113 , C12N15/625 , C12N15/8202 , C12N15/8223 , C12N15/8287 , C12Q1/68 , C12Q1/6895 , C12Q2525/207 , C12Q2600/13
Abstract: The invention provides nucleotide sequences and amino acid sequences of the Dipgene as well as (functional) homologues, fragments and variants thereof, which provides diplospory as a part of apomixis. Also diplospory plants and methods for making these are provided, as are methods of using these, and methods of making apomictic seed.
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公开(公告)号：US09896721B2
公开(公告)日：2018-02-20
申请号：US15350441
申请日：2016-11-14
Applicant: Keygene N.V.
CPC classification number: G06F19/22 , C12N15/1065 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6858 , C12Q1/6874 , C12Q1/6883 , C12Q2600/156 , C40B30/04 , C40B30/10 , C12Q2539/107 , C12Q2531/113 , C12Q2525/191
Abstract: The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe.
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公开(公告)号：US20180016631A1
公开(公告)日：2018-01-18
申请号：US15539273
申请日：2015-12-23
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia VAN EIJK
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12N15/10 , C12N15/1093 , C12N15/64 , C12N15/66 , C12Q1/68 , C12Q1/6806 , C12Q1/6809 , C12Q1/683 , C12Q1/6855 , C12Q1/6869 , C12Q2521/301 , C12Q2522/101 , C12Q2525/191 , C12Q2563/179 , C12Q2525/131 , C12Q2525/155 , C12Q2535/122
Abstract: Disclosed is a method suitable for (long-range) mate pair sequencing wherein the mate pairs are located within a certain distance from each other on the same nucleotide sequence. By ligating a DNA fragment into an identifier section—containing backbone, a digestable circularized construct is provided to which adaptors can be ligated after digestion. Amplification yields amplicons that contain a combination of the identifier section with the terminal part of the fragments. The fragments are subsequently mated to each other to obtain a mated pair by identifying the corresponding identifier section in both amplicons. The mated pairs can be used in the construction of genome scaffolds or in the generation of draft genome sequences.
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Patent Agency Ranking
公开(公告)号：US20170356042A1
公开(公告)日：2017-12-14
申请号：US15683252
申请日：2017-08-22
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia VAN EIJK , Anker Preben SØRENSEN , Marco Gerardus Maria VAN SCHRIEK
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6876 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16 , C12Q2565/301 , C12Q2535/138 , C12Q2535/122 , C12Q2527/107
Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
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公开(公告)号：US20170342486A1
公开(公告)日：2017-11-30
申请号：US15674126
申请日：2017-08-10
Applicant: Keygene N.V.
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q1/6827 , C12Q1/6846 , C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , C12Q1/6869 , C12Q2600/13 , G16B30/00 , C12Q2525/155 , C12Q2563/179 , C12Q2563/155 , C12Q2537/143
Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.
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公开(公告)号：US20170306347A1
公开(公告)日：2017-10-26
申请号：US15627725
申请日：2017-06-20
Applicant: Keygene N.V.
Inventor： Adrianus Johannes VAN TUNEN
IPC: C12N15/82 , C12Q1/68 , C07K14/415
CPC classification number: C12N15/8273 , C07K14/415 , C12Q1/6895
Abstract: The present invention provides use of a plant gene JAZ5a for improving drought-resistance of a plant. It further provides a method for improving the drought-resistance of a plant, comprising enhancing the expression or activity of Jaz5A in said plant.
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公开(公告)号：US20170166911A1
公开(公告)日：2017-06-15
申请号：US15384644
申请日：2016-12-20
Applicant: Keygene N.V.
IPC: C12N15/82
CPC classification number: C12N15/8206 , C12N15/8201 , C12N15/8218 , C12N15/8266
Abstract: The invention relates to a method for the introduction of one or more molecules of interest in a plant cell protoplast by providing plant cell protoplasts, performing a first transfection of the plant cell protoplast with a composition that is capable of altering the regulation of one or more pathways selected from the group consisting of Mismatch Repair System and Non-Homologous End Joining and/or a composition that is capable of introducing DSBs, performing a second transfection of the plant cell protoplast with one or more molecules of interest such as mutagenic oligonucleotides and allowing the cell wall to form.
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公开(公告)号：US20170159120A1
公开(公告)日：2017-06-08
申请号：US15434801
申请日：2017-02-16
Applicant: Keygene N.V.
CPC classification number: C12Q1/6874 , C12Q1/6806 , C12Q1/6827 , C12Q1/6846 , C12Q1/6851 , C12Q1/6855 , C12Q1/6858 , C12Q1/6869 , C12Q2600/13 , G06F19/22 , C12Q2525/155 , C12Q2563/179 , C12Q2563/155 , C12Q2537/143
Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.
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