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    High performance inverter charger system
    141.
    发明申请
    High performance inverter charger system 失效
    高性能逆变器充电器系统

    公开(公告)号：US20070081372A1

    公开(公告)日：2007-04-12

    申请号：US11544104

    申请日：2006-10-06

    Applicant: James Xiong Zeng ,  David Liu

    Inventor： James Xiong Zeng ,  David Liu

    IPC: H02M7/5387

    CPC classification number: H02M7/5387

    Abstract: Techniques for high performance inverter charger systems are described herein. In one embodiment, a power supply system includes, but is not limited to, an inverter to generate an AC (alternating current) output based on a DC (direct current) input, a current sensing circuit coupled to the inverter to sense an amount of current drawn from the inverter, and a microcontroller coupled to the inverter and the current sensing circuit to reduce the AC output of the inverter according to a predetermined algorithm stored within the microcontroller, in response to a detection that the amount of current drops below a predetermined threshold. Other methods and apparatuses are also described.

    Abstract translation: 本文描述了用于高性能逆变器充电器系统的技术。 在一个实施例中，电源系统包括但不限于基于DC（直流）输入产生AC（交流）输出的逆变器，耦合到逆变器的电流感测电路以感测 响应于检测到电流量下降到低于预定值的电流，耦合到逆变器和电流感测电路的微控制器根据存储在微控制器内的预定算法减少逆变器的AC输出， 阈。 还描述了其它方法和装置。

    Ligand-dependent protein splicing
    145.
    发明申请
    Ligand-dependent protein splicing 有权
    配体依赖性蛋白质拼接

    公开(公告)号：US20050272072A1

    公开(公告)日：2005-12-08

    申请号：US11093808

    申请日：2005-03-30

    Applicant: David Liu ,  Allen Buskirk

    Inventor： David Liu ,  Allen Buskirk

    IPC: C07H21/04 ,  C07K14/35 ,  C07K14/395 ,  C07K14/72 ,  C12N9/14 ,  C12N15/62 ,  C12Q1/68

    CPC classification number: C07H21/04 ,  C07K14/35 ,  C07K14/395 ,  C07K2319/90 ,  C12N9/14 ,  C12N15/62

    Abstract: Ligand-dependent inteins allow for modulation of a protein's activity in vivo. Upon binding of the ligand to the ligand-dependent intein inserted into the protein of interest, the hybrid protein undergoes protein splicing removing the intein. The activity of the spliced protein is then restored. A 4-hydroxytamoxifen-dependent intein based on the M. tuberculosis RecA intein is prepared and demonstrated in a variety of exteins contexts. The invention provides a system for engineering other ligand-dependent inteins and using them, including the ligand-dependent inteins themselves, hybrid proteins with the inserted ligand-dependent inteins, polynucleotides encoding inteins and hybrid proteins, and engineered cells. Kits with the materials and reagents necessary for preparing and using ligand-dependent inteins are also included.

    Abstract translation: 依赖于配体的内含肽允许在体内调节蛋白质的活性。 当配体与插入目的蛋白质中的配体依赖内含肽结合时，杂交蛋白经蛋白质剪接除去内含肽。 然后恢复剪接的蛋白质的活性。 基于结核分枝杆菌RecA内含肽的4-羟基他莫昔芬依赖性内含肽在各种exteins背景下制备和证实。 本发明提供了一种用于工程化其他配体依赖性内含肽并使用它们的系统，包括配体依赖性内含肽本身，与插入的配体依赖内含肽的杂交蛋白，编码内含肽和杂交蛋白的多核苷酸和工程细胞。 还包括使用制备和使用配体依赖内含肽所必需的材料和试剂的试剂盒。

    Directed evolution of proteins
    146.
    发明申请
    Directed evolution of proteins 有权
    蛋白质的定向进化

    公开(公告)号：US20050260655A1

    公开(公告)日：2005-11-24

    申请号：US11107335

    申请日：2005-04-15

    Applicant: David Liu ,  Joshua Bittker ,  Jane Liu

    Inventor： David Liu ,  Joshua Bittker ,  Jane Liu

    IPC: C12N15/09 ,  C12N15/10 ,  C12N15/62 ,  C12P21/06 ,  C12Q1/68

    CPC classification number: C12N15/62 ,  C12N15/1027 ,  C12N15/1058 ,  C12Q1/6806 ,  C12Q2533/107 ,  C12Q2525/203 ,  C12Q2521/301

    Abstract: Disclosed is a method of altering a nucleic acid such as RNA or DNA. The method comprises fragmenting a parent nucleic acid strand to generate nucleic acid fragments. At least a subset of the fragments are ligated to generate shuffled nucleic acid strands. A selected strand is identified from the shuffled nucleic acid strands for a criterion. The methods of the invention can also be used to diversify proteins.

    Abstract translation: 公开了一种改变核酸如RNA或DNA的方法。 该方法包括将母体核酸链断裂以产生核酸片段。 连接片段的至少一个子集以产生洗牌的核酸链。 从洗牌的核酸链中鉴定出选择的链，用于标准。 本发明的方法也可用于使蛋白多样化。

    Multifunction vehicular surface cleaning system
    147.
    发明申请
    Multifunction vehicular surface cleaning system 审中-公开
    多功能车辆表面清洗系统

    公开(公告)号：US20050211280A1

    公开(公告)日：2005-09-29

    申请号：US10809746

    申请日：2004-03-25

    Applicant: David Liu

    Inventor： David Liu

    IPC: B08B3/02 ,  B60S3/04

    CPC classification number: B08B3/028 ,  A46B11/06 ,  B05B1/3026 ,  B05B7/0408 ,  B05B7/1209 ,  B05B7/2445 ,  B05B9/01 ,  B08B3/026 ,  B08B2203/0217 ,  B60S3/047

    Abstract: The present invention provides a multifunction vehicular surface cleaning system comprised of pipe, soap box and switcher; wherein, said soap box is located on the outside of said pipe at the inlet end of the pipe with the its outlet; said switcher is correspondently set upon the outlet of the soap box and the inlet of the pipe, so that by turning said switcher the system can be shifted in cut-off said input pipe with soap box and output pipe, or cut-in the input pipe to output pipe directly only, or cut-in the input pipe to both the output pipe and the soap box, in order to carry out three working states of cut-off, or spraying clear water or soap water. A hollow brush is connected to the outlet of output pipe in socket joint, and there are some water gates built on bottom base of the brush for facilitating to cleaning operation directly. Said cleaning system is very convenient in shifting operation as changing functions, meanwhile the leak tightness is good, the cleaning operation is also convenient.

    Abstract translation: 本发明提供一种由管，肥皂盒和切换器组成的多功能车辆表面清洁系统; 其特征在于，所述肥皂盒在其出口处在管道的入口端处位于所述管道的外侧; 所述切换器相应地设置在肥皂盒的出口和管道的入口上，使得通过转动所述切换器，系统可以用肥皂盒和输出管切断所述输入管，或切入输入 管道直接输出管道，或将输入管切入输出管和肥皂盒，以进行三种工作状态的切断，或喷洒清水或肥皂水。 中空刷连接到插座接头的输出管出口，在刷子的底部有一些浇水口，便于直接清洗操作。 所述清洗系统作为变换功能在变速操作中非常方便，同时泄漏密封性好，清洗操作也方便。

    Health care shoe
    149.
    发明授权
    Health care shoe 失效
    保健鞋

    公开(公告)号：US06360457B1

    公开(公告)日：2002-03-26

    申请号：US09607516

    申请日：2000-06-28

    Applicant: Yi-Ming Qui ,  David Liu ,  Joanne Yang

    Inventor： Yi-Ming Qui ,  David Liu ,  Joanne Yang

    IPC: A61F514

    CPC classification number: A43B7/146 ,  A43B1/0054 ,  A43B7/00

    Abstract: A health care shoe, which can continuously provide adequate magnetic field arranged to penetrate through the wearer's foot for improving the wearer's blood circulation and metabolism, includes a sole; an insole placed on top the sole, which has a plurality of magnet cavities formed and distributed thereon; a shoe body rigidly attached on top of the sole to define a foot chamber between the shoe body and the insole; a plurality of base magnet elements fittingly received in the magnet cavities respectively provided on the insole; and a plurality of upper magnet elements embedded inside the shoe body with respect to the positions of the base magnet elements located on the insole, wherein each of the upper magnet elements is arranged to constitute a transversal magnetic field penetrating through the foot chamber and flowing towards one of the base magnet elements. Moreover, the sole further has at least a medicine receiving cavity to receive a medicine unit which contains a medicine tablet made of painkiller or Chinese medical herb being incased therein. The medicine unit further includes a bottom magnet element positioned thereunder so as to produce magnetic filed around the medicine unit.

    Abstract translation: 可以连续地提供足够的磁场穿过穿着者的足部以改善佩戴者的血液循环和新陈代谢的保健鞋，包括鞋底; 将鞋垫放置在鞋底上，其上形成有多个形成并分布在其上的磁体腔; 鞋体刚性地附接在鞋底的顶部上以在鞋身和鞋垫之间限定脚室; 分别设置在所述鞋垫上的所述磁体空腔中的多个基体磁体， 以及相对于位于鞋垫上的基部磁体元件的位置，嵌入鞋体内部的多个上部磁体元件，其中每个上部磁体元件被布置成构成穿过底座室的横向磁场并向 一个基本的磁铁元素。 此外，鞋底还具有至少一个药物容纳腔，以容纳药物单元，该药物单元含有由止痛药制成的药片或其中含有的中草药。 药物单元还包括位于其下方的底部磁体元件，以便产生药物单元周围的磁场。

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