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    Optical interferometer, data acquisition device, and data acquisition method
    168.
    发明授权
    Optical interferometer, data acquisition device, and data acquisition method 有权

    公开(公告)号：US09599454B2

    公开(公告)日：2017-03-21

    申请号：US14348495

    申请日：2012-09-27

    Applicant: TOKYO UNIVERSITY OF SCIENCE FOUNDATION

    Inventor： Hiroharu Yui

    IPC: G01J3/45 ,  G01J3/44 ,  G01B9/02 ,  G01J3/453 ,  G01N21/65 ,  G01J9/04

    CPC classification number: G01B9/02041 ,  G01J3/44 ,  G01J3/4535 ,  G01J9/04 ,  G01N21/65 ,  G01N2021/655

    Abstract: An optical interferometer includes: a light source that emits a coherent first beam and a second beam that has a frequency difference corresponding to the natural frequency of a target molecule; amplitude modulating means that modulates the amplitude of the second beam; splitting means that splits the first beam into a reference beam and a first applied beam; optical path length adjusting means that adjusts the optical path length of the reference beam; and detecting means that detects an interference pattern between the reference beam and the first beam (a signal beam) that has experienced a stimulated Raman loss or gain in accordance with the amplitude modulation as a result of the frequency difference resonating with the target molecule when the first applied beam and a second applied beam (the amplitude modulated second beam) have been applied to a measurement position of an object.

    Method for detecting protein that interacts with target substance
    170.
    发明授权
    Method for detecting protein that interacts with target substance 有权
    检测与目标物质相互作用的蛋白质的方法

    公开(公告)号：US09513295B2

    公开(公告)日：2016-12-06

    申请号：US14347707

    申请日：2012-09-28

    Applicant: TOKYO UNIVERSITY OF SCIENCE FOUNDATION

    Inventor： Etsuko Miyamoto ,  Shigeo Fujimori

    IPC: C40B30/04 ,  G01N33/68 ,  C12N15/10 ,  C12Q1/68

    CPC classification number: G01N33/6818 ,  C12N15/1034 ,  C12N15/1062 ,  C40B30/04

    Abstract: The present invention provides a method for detecting an interaction, which method can solve not only the problem of false negatives but also the problem of false positives. This method is a method for detecting a protein(s) that interact(s) with a target substance(s), the method comprising repeating a (1) transcription step, (2) assignment step, (3) selection step, and (4) amplification step, wherein: (a) in each of a plurality of times of preparation of a cDNA library among the initial preparation of a cDNA library and the round(s) of preparation of a cDNA library in the later amplification step(s), the cDNA library is prepared using a primer(s) having a sequence(s) specific to the time of preparation; (b) the cDNA libraries prepared using the primers having sequences specific to the times of preparation are mixed, and sequences in the cDNA library mixture are determined; (c) the determined sequences are subjected to measurement of the number(s) of molecules encoding the same candidate protein(s) for each time of preparation based on the sequence(s) specific to the time of preparation; and (d) a candidate protein(s) encoded by a molecule(s) that significantly increase(s) as the preparation rounds proceed is/are detected as the protein(s) that interact(s) with the target substance(s).

    Abstract translation: 本发明提供一种检测相互作用的方法，该方法不仅可以解决假阴性的问题，而且可以解决假阳性的问题。 该方法是用于检测与目标物质相互作用的蛋白质的方法，该方法包括重复（1）转录步骤，（2）分配步骤，（3）选择步骤和（ 4）扩增步骤，其中：（a）在cDNA文库的初始制备中的cDNA文库的制备中的每一次中，以及后续扩增步骤（s）中cDNA文库的制备循环 ），使用具有与制备时间相关的序列的引物制备cDNA文库; （b）使用具有与制备时间特异性序列的引物制备的cDNA文库混合，并确定cDNA文库混合物中的序列; （c）基于制备时间特有的序列，对每个制备时间对测定的序列进行编码相同候选蛋白质的分子的数目的测定; 和（d）当与制备循环进行时显着增加的分子编码的候选蛋白质被检测为与目标物质相互作用的蛋白质， 。

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