公开(公告)号：US20140327349A1

公开(公告)日：2014-11-06

申请号：US14266501

申请日：2014-04-30

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Jeffry M. Ceremony ,  Tom McCollum

IPC: B01L7/00

CPC classification number: B01L7/52 ,  B01L2300/041 ,  B01L2300/046 ,  B01L2300/0829 ,  B01L2300/1827

Abstract: A drawer apparatus having a drawer chassis and a drawer adapted to move relative to the drawer chassis from a closed position to an open position. The drawer has first and second lateral sides. A lid assembly is moveable from an upward position to a downward position relative to the drawer chassis. The lid assembly has first and second roller members. A first lever arm is rotatable about a first pivot on the first lateral side. The first lever arm has a first surface for moveably engaging with the first roller member. A second lever arm is rotatable about a second pivot on the second lateral side. The second lever arm has a second surface for moveably engaging with the second roller. A handle connects the first and second lever arms. The handle is adapted to move the first and second lever arms respectively about the first and second pivots to move the lid assembly from the downward position to the upward position.

Abstract translation: 一种抽屉设备，其具有抽屉底盘和适于相对于抽屉底盘从关闭位置移动到打开位置的抽屉。 抽屉有第一和第二侧面。 盖组件可相对于抽屉底座从向上位置移动到向下位置。 盖组件具有第一和第二辊构件。 第一杠杆臂可围绕第一侧面的第一枢轴转动。 第一杠杆臂具有用于与第一辊构件可移动地接合的第一表面。 第二杠杆臂可绕第二横向侧上的第二枢轴旋转。 第二杠杆臂具有用于与第二辊可动地接合的第二表面。 手柄连接第一和第二杠杆臂。 手柄适于分别围绕第一和第二枢轴移动第一和第二杠杆臂，以将盖组件从向下位置移动到向上位置。

公开(公告)号：US20140293412A1

公开(公告)日：2014-10-02

申请号：US14262559

申请日：2014-04-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael Griffin ,  Daniel Yue Man Chu ,  Tom McCollum

IPC: G02B21/06

CPC classification number: G02B21/06 ,  G02B21/125 ,  G02B21/16 ,  G02B21/24 ,  G02B21/365

Abstract: A system, apparatus and method for using modular microscopes is disclosed. Connecting the housings of the individual microscope modules provide the structural framework of the modular microscope. Furthermore, the modular microscope can include specialized software, the distribution and use of which can be controlled using security keys or identifiers stored on one or more of the microscope modules. The security keys and identifiers can be based on calibration data associated with the physical, electrical, or optical properties of one of more of the modules. The illumination modules disclosed provide for selectable wavelengths and controllable levels of output illumination for both bright field and dark field illumination.

Abstract translation: 公开了一种使用模块显微镜的系统，装置和方法。 连接各个显微镜模块的外壳提供了模块化显微镜的结构框架。 此外，模块化显微镜可以包括专门的软件，可以使用存储在一个或多个显微镜模块上的安全密钥或标识符来控制其分布和使用。 安全密钥和标识符可以基于与多个模块之一的物理，电或光学特性相关联的校准数据。 所公开的照明模块为亮场和暗场照明提供了可选择的波长和输出照明的可控电平。

公开(公告)号：US20140260970A1

公开(公告)日：2014-09-18

申请号：US14209974

申请日：2014-03-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Nathan Wrench ,  William Mainwaring-Burton

IPC: B01D19/00

CPC classification number: B01D19/0031

Abstract: Disclosed herein is an apparatus for debubbling or degassing a liquid. The apparatus includes a tube with a hollow lumen and a gas-permeable, liquid-impermeable wall surrounding the lumen. Also disclosed is a method for performing the debubbling or degassing, wherein the liquid is passed through the tube and gas is allowed to escape through the wall of the tube. The application additionally discloses a system for debubbling or degassing a liquid, wherein a pump is connected to the upstream end of the tube and a device to impede the flow of liquid out the tube is connected to the downstream end.

Abstract translation: 本文公开了一种用于对液体进行脱泡或脱气的装置。 该装置包括具有中空腔的管和围绕内腔的透气的液体不可渗透的壁。 还公开了一种用于进行脱泡或脱气的方法，其中液体通过管，并且允许气体通过管的壁逸出。 该应用还公开了一种用于对液体进行脱泡或脱气的系统，其中泵连接到管的上游端，并且阻止液体流出管的装置连接到下游端。

公开(公告)号：US20140228239A1

公开(公告)日：2014-08-14

申请号：US14173562

申请日：2014-02-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam M. McCoy ,  Svilen Tzonev ,  Frank Hsiung

IPC: G01N33/566

CPC classification number: C12Q1/00 ,  G01N33/542 ,  G01N33/581

Abstract: The present invention provides methods of detecting a target molecule in a sample comprising incubating the sample with two or more detectably labeled probes, partitioning the sample into multiple partitions, and detecting the presence of the two or more probes in the same partition.

Abstract translation: 本发明提供了检测样品中靶分子的方法，包括用两个或多个可检测标记的探针孵育样品，将样品分成多个分区，以及检测两个或更多个探针在同一分区中的存在。

公开(公告)号：US20140220577A1

公开(公告)日：2014-08-07

申请号：US13924353

申请日：2013-06-21

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： YAN WANG ,  PETER VANDER HORN ,  LEI XI

IPC: C12Q1/68

CPC classification number: C12N15/10 ,  C07K14/195 ,  C07K2319/00 ,  C07K2319/80 ,  C12N9/1252 ,  C12N15/62 ,  C12P19/34 ,  C12Q1/686 ,  C12Y207/07007

Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.

Abstract translation: 本发明提供使用改进的核酸聚合酶的产生来测序和进行聚合酶反应的方法。 改进是以提高聚合酶的持续性的方式将序列非特异性核酸结合结构域与酶的融合。

公开(公告)号：US20140157859A1

公开(公告)日：2014-06-12

申请号：US14100397

申请日：2013-12-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Adam Darmstadt ,  Michael Waite ,  Theresa Forni

IPC: G01N33/49

CPC classification number: G01N35/00693 ,  G01N35/0095 ,  G01N2030/047 ,  G01N2035/00702

Abstract: An improved calibration process for a medical testing machine. The machine automatically recognizes that a package of calibration material has been inserted into it, and performs a calibration sequence to ascertain a calibration parameter to be used in performing future tests with the medical testing machine. The calibration package may include machine-readable indicators that the package is to be used for calibration, and of a calibration setpoint of a calibration material in the package. A calibration material may be stored in a lyophilized state in the package, and the medical testing machine may automatically reconstitute the lyophilized material.

Abstract translation: 用于医疗测试机器的改进的校准过程。 机器自动识别出已经插入校准材料包，并执行校准顺序，以确定用于在医学测试机器中进行将来测试中使用的校准参数。 校准包可以包括用于校准的包装的机器可读指示器以及包装中的校准材料的校准设定点。 校准材料可以以冻干状态存储在包装中，并且医学检测机器可以自动重构冻干的材料。

公开(公告)号：US08715476B2

公开(公告)日：2014-05-06

申请号：US12963430

申请日：2010-12-08

Applicant: Matthew Latham ,  William Strong ,  Nikolas Chmiel ,  Jeff Xu ,  Robert Iovanni

Inventor： Matthew Latham ,  William Strong ,  Nikolas Chmiel ,  Jeff Xu ,  Robert Iovanni

IPC: G01N27/447

CPC classification number: G01N27/44739 ,  B01D57/02 ,  G01N27/44782

Abstract: Electrotransfer is performed in an instrument that receives electroblotting cassettes and that contains an integrated power supply, controls, and a display that allows the user to monitor and control each of a plurality of cassettes individually through electrical contacts within the housing that mate with corresponding electrical contacts on the cassettes.

Abstract translation: 电传递在接收电印箱的仪器中进行，并且包含集成的电源，控制和显示器，其允许用户通过壳体内的电接触单独地监测和控制多个盒中的每一个，从而与相应的电触点 在盒子上

公开(公告)号：US08709762B2

公开(公告)日：2014-04-29

申请号：US13039233

申请日：2011-03-02

Applicant: Benjamin J. Hindson

Inventor： Benjamin J. Hindson

IPC: C12N9/12 ,  C12P19/34

CPC classification number: C12N9/12 ,  C12P19/34

Abstract: System, including methods, apparatus, compositions, and kits, for making and using compound droplets of a multiple emulsion to supply an amplification reagent, such as a heat-stable DNA polymerase or DNA ligase, to an aqueous phase in which the compound droplets are disposed. The compound droplets may be induced to supply the amplification reagent by heating the multiple emulsion, to achieve hot-start amplification.

Abstract translation: 用于制备和使用多重乳液的复合液滴的方法，装置，组合物和试剂盒的系统将诸如热稳定的DNA聚合酶或DNA连接酶的扩增试剂提供到水相中，其中复合液滴是 处置。 可以通过加热多重乳液来诱导复合液滴供应扩增试剂，以实现热启动扩增。

公开(公告)号：US20140057293A1

公开(公告)日：2014-02-27

申请号：US13973596

申请日：2013-08-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Thomas R. Berkelman ,  Lisandra Martin ,  Rhiannon Jones ,  John Walker, II

IPC: G01N33/543 ,  C08F220/60

CPC classification number: G01N27/44747 ,  B01D15/3823 ,  C07D255/02 ,  C07F3/06 ,  C07F13/005 ,  C08F220/56 ,  C08F220/60 ,  G01N33/54306 ,  Y10T436/143333 ,  Y10T436/163333

Abstract: Conjugates of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes with a variety of conjugating members are used in the formation of dinuclear metal complexes which bind to phosphate esters. By virtue of their conjugated forms, the complexes are incorporated into chromatographic media, affinity binding reagents, and dyes, which make the complexes useful in a wide range of assays, separations, and purifications. In addition, dinuclear metal complexes of 1,3-bis(1,4,7-triazacyclonon-1-yl)-2-hydroxypropanes that are not so conjugated are used in the detection of phosphate esters of biological species by either MALDI-TOF mass spectrometry or by dye displacement.

Abstract translation: 将1,3-双（1,4,7-三氮杂环壬-1-烯）-2-羟基丙烷与多种共轭成员的共轭物用于形成与磷酸酯结合的双核金属络​​合物。 由于它们的共轭形式，复合物被并入到色谱介质，亲和力结合试剂和染料中，这使复合物可用于广泛的测定，分离和纯化。 此外，不结合的1,3-双（1,4,7-三氮杂环壬烷-1-基）-2-羟基丙烷的双核金属络​​合物用于通过MALDI-TOF检测生物物种的磷酸酯 质谱或染料置换。

公开(公告)号：US20140042027A1

公开(公告)日：2014-02-13

申请号：US13961626

申请日：2013-08-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Christopher Belisle

IPC: G01N27/447

CPC classification number: G01N27/44747 ,  G01N27/44713 ,  G01N27/44721 ,  G01N27/44726 ,  G01N27/44739

Abstract: Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products.

Abstract translation: 在凝胶中电泳分离的蛋白质通过将卤素取代的有机化合物引入凝胶两端的一个或两个电极缓冲溶液中衍生而产生荧光发射。 使用的卤素取代的化合物是在缓冲溶液和凝胶的pH下具有电荷的化合物，并且化合物上的电荷的极性使得化合物在电泳影响下从电极缓冲液迁移到凝胶中 随着蛋白质迁移到凝胶中。 一旦蛋白质被分离并分布在凝胶中并且凝胶被卤素取代的化合物完全渗透，则用紫外线照射凝胶以通过蛋白质上的色氨酸残基诱导卤素取代的化合物与蛋白质之间的反应 ，产生荧光反应产物。