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    Generation of Chemical Building Blocks from Plant Biomass by Selective Depolymerization
    18.
    发明申请
    Generation of Chemical Building Blocks from Plant Biomass by Selective Depolymerization 有权
    通过选择性解聚从植物生物量生成化学构件

    公开(公告)号：US20090004697A1

    公开(公告)日：2009-01-01

    申请号：US12138096

    申请日：2008-06-12

    Applicant: Andre Koltermann ,  Ulrich Kettling ,  Thomas Bruck ,  Markus Rarbach

    Inventor： Andre Koltermann ,  Ulrich Kettling ,  Thomas Bruck ,  Markus Rarbach

    IPC: C12P19/00

    CPC classification number: C12N9/2434 ,  C12N9/0061 ,  C12P19/02 ,  C12P19/12 ,  C12P19/14 ,  C12P19/16 ,  C12P19/18 ,  C12P19/20 ,  C12P19/22 ,  C12P19/24 ,  C13B35/005 ,  C13K1/02 ,  C13K1/06 ,  C13K13/002

    Abstract: The invention concerns a method for the enzymatic treatment of raw polymeric feedstock comprising the following steps: (a) preferably separation of soluble components from the raw polymeric feedstock, (b) treating the raw polymeric feedstock with an enzyme system in order to liberate defined soluble monomeric or oligomeric building blocks from the insoluble raw polymeric feedstock; and (c) separating the defined monomeric or oligomeric building blocks produced in step b) from the remainder of the raw polymeric feedstock. Preferably, the enzyme system used in step b) contains not more than 50%, preferably not more than 20%, more preferably not more than 10%, more preferably not more than 5%, more preferably not more than 2%, more preferably not more than 1% of other enzyme activities apart from the enzyme activity resulting in liberation of said defined monomeric or oligomeric building blocks from the raw polymeric feedstock according to step b). Further aspects of the invention concern the use of “less pure” and thus less costly enzyme systems in subsequent enzymatic treatment steps and methods for determining the optimum sequence of enzymatic treatment steps by analysis of the raw polymeric feedstock used.

    Abstract translation: 本发明涉及用于酶处理原料聚合物原料的方法，包括以下步骤：（a）优选将可溶性组分与原料聚合原料分离，（b）用酶体系处理原料聚合物原料，以释放限定的可溶性 来自不溶性原料聚合原料的单体或低聚结构单元; 和（c）将步骤b）中制备的限定的单体或低聚结构单元与原始聚合原料的其余部分分离。 优选地，步骤b）中使用的酶系统含有不超过50％，优选不超过20％，更优选不超过10％，更优选不超过5％，更优选不超过2％ 不超过1％的其它酶活性，除了酶活性，导致根据步骤b）从所述原料聚合物原料释放所述限定的单体或低聚结构单元。 本发明的另外方面涉及在随后的酶处理步骤中使用“较不纯”且因此较低成本的酶体系，以及通过分析所用原料聚合物原料来确定酶处理步骤的最佳顺序的方法。

    Method for the production of biopolymers with modified properties
    20.
    发明授权
    Method for the production of biopolymers with modified properties 有权
    生产具有改性特性的生物聚合物的方法

    公开(公告)号：US06821758B1

    公开(公告)日：2004-11-23

    申请号：US09708497

    申请日：2000-11-09

    Applicant: Andre Koltermann ,  Ulrich Kettling ,  Manfred Eigen

    Inventor： Andre Koltermann ,  Ulrich Kettling ,  Manfred Eigen

    IPC: C12P1934

    CPC classification number: C12N15/1027

    Abstract: A method for the production of biopolymers with modified properties wherein at least one cycle comprising the following steps is completed: (a) providing a population of single-stranded polynucleotide molecules, wherein individual polynucleotide molecules comprise homologous and heterologous sequence segments and wherein individual ones of said single-stranded polynucleotide molecules can form double-stranded polynucleotide molecules with other ones of said single-stranded polynucleotide molecules within said population; (b) forming double-stranded polynucleotide molecules from the population of single-stranded polynucleotide molecules provided according to step (a) comprising double-stranded polynucleotide molecules with different heterologous sequence segments; (c) partially and exonucleolytically degrading the single-strands of the double-stranded polynucleotide molecules produced according to step (b); and (d) temple-directed single-stand synthesizing the degraded ends of the partially degraded double strand produced according to step (c), wherein steps (c) and (d) may be carried out sequentially or contemporaneously.

    Abstract translation: 一种用于生产具有改进性质的生物聚合物的方法，其中完成包括以下步骤的至少一个循环：（a）提供一群单链多核苷酸分子，其中单个多核苷酸分子包含同源和异源序列区段， 所述单链多核苷酸分子可以与所述群体内的所述单链多核苷酸分子中的其它单链多核苷酸分子形成双链多核苷酸分子;（b）从根据步骤（I）提供的单链多核苷酸分子群体形成双链多核苷酸分子 a）包含具有不同异源序列片段的双链多核苷酸分子;（c）部分和外核解离降解根据步骤（b）制备的双链多核苷酸分子的单链; 和（d）合成根据步骤（c）制备的部分降解的双链的降解末端的神经导向单支，其中步骤（c）和（d）可以顺序或同时进行。

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