Method and Kit for Extracting Cells from Soil Samples

    公开(公告)号:US20240044755A1

    公开(公告)日:2024-02-08

    申请号:US18166739

    申请日:2023-02-09

    CPC classification number: G01N1/34 G01N1/10 G01N2001/1025

    Abstract: A method of extracting viable bacterial cells from a soil sample by mixing a dispersant-surfactant solution with a soil sample to form a soil slurry, adding the soil slurry to a centrifuge tube containing a density gradient medium, centrifuging the centrifuge tube to form a solvent layer above the density gradient medium, wherein the solvent layer comprises viable bacterial cells, extracting the solvent layer containing the viable bacterial cells, combining the extracted solvent layer with a PBS solution to form a PBS-cell mixture, filtering the PBS-cell mixture to form a cell filtrate, depositing the cell filtrate into a second centrifuge tube containing a quantity of the density gradient medium, centrifuging the second centrifuge tube to form a second solvent layer comprising the viable bacterial cells, and extracting the second solvent layer from the second centrifuge medium to form a second cell filtrate comprising the viable bacterial cells.

    Dead Detector Element Detection
    19.
    发明申请

    公开(公告)号:US20230079742A1

    公开(公告)日:2023-03-16

    申请号:US17930294

    申请日:2022-09-07

    Abstract: A method comprises: performing training and testing of an initial machine model to create a final machine model, wherein the training and testing use focal loss; performing detection of dead detector elements in a digital detector of a second digital radiographic (DR) imaging system using the final machine model; and determining whether to replace or keep the digital detector based on the detection. An apparatus comprises: a memory; and a processor coupled to the memory and configured to: perform training and testing of an initial machine model to create a final machine model, wherein the training and testing use focal loss; perform detection of dead detector elements in a digital detector of a second digital radiographic (DR) imaging system using the final machine model; and determine whether to replace or keep the digital detector based on the detection.

    Apparatus and method for improving air quality in street canyons

    公开(公告)号:US11598540B2

    公开(公告)日:2023-03-07

    申请号:US17501410

    申请日:2021-10-14

    Inventor: Lee A. Fithian

    Abstract: A double skin façade (DSF) for a building is disclosed. The DSF has a first façade, a second façade, and a gap extending therebetween. Movement of air is driven in an upward direction toward an upper end of the DSF, causing movement of air contaminants from a lower end of the DSF to the upper end of the DSF, and thereby enhancing the air quality of the street canyon by removing air contaminants from a street level adjacent the building. Also disclosed is a building having a DSF, and a method of enhancing air quality in a street canyon by constructing a DSF on a building.

Patent Agency Ranking