公开(公告)号：US20100257635A1

公开(公告)日：2010-10-07

申请号：US12752175

申请日：2010-04-01

Applicant: Knut Meyer ,  Howard Glenn Damude ,  John D. Everard ,  Kevin G. Ripp ,  Kevin L. Stecca

Inventor： Knut Meyer ,  Howard Glenn Damude ,  John D. Everard ,  Kevin G. Ripp ,  Kevin L. Stecca

IPC: A01H5/00 ,  C12N15/82

CPC classification number: C12N15/8247 ,  C12N15/8201

Abstract: A recombinant DNA construct comprising a polynucleotide encoding an ODP1 polypeptide operably linked to a sucrose synthase 2 promoter where this construct can be used to increase oil content in the seeds of a cruciferous oilseed plant while maintaining normal germination is disclosed. A method for increasing oil content in the seeds of a cruciferous oilseed plant while maintaining normal germination using this construct is also disclosed.

Abstract translation: 公开了一种重组DNA构建体，其包含编码与蔗糖合酶2启动子可操作地连接的ODP1多肽的多核苷酸，其中该构建体可用于增加十字花科油籽植物的种子中的油含量，同时保持正常发芽。 还公开了一种在使用该构建体保持正常发芽的同时增加十字花科油籽植物的种子中的油含量的方法。

公开(公告)号：US20100162435A1

公开(公告)日：2010-06-24

申请号：US12530481

申请日：2008-04-16

Applicant: Howard Glenn Damude ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Quinn Qun Zhu

IPC: A01H1/00 ,  C07H21/00 ,  C12N15/74 ,  C12N5/10 ,  A01H5/00 ,  C07C57/02

CPC classification number: C12N9/1029 ,  A23D9/00 ,  A23D9/007 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  A23V2250/1862 ,  A23V2250/187 ,  A23V2250/1868

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) and using these delta-9 elongases in plants.

Abstract translation: 分离的核酸片段和包含编码Δ-9延伸酶的片段的重组构建物以及制备长链多不饱和脂肪酸（PUFA）的方法并在植物中使用这些δ-9延伸酶。

公开(公告)号：US20100099764A1

公开(公告)日：2010-04-22

申请号：US12524363

申请日：2008-02-12

Applicant: Howard Glenn Damude ,  Anthony J. Kinney

Inventor： Howard Glenn Damude ,  Anthony J. Kinney

IPC: A61K31/20 ,  A01H5/00 ,  A23L1/30 ,  A23L1/36

CPC classification number: C12N15/8247 ,  A23D9/00 ,  A23K10/30 ,  A23K20/163 ,  A23K50/80 ,  A23L33/12 ,  C12N9/0083 ,  C12N9/1029 ,  Y02A40/818

Abstract: Oilseed plants which have been transformed to produce arachidonic acid, recombinant constructs used in such transformations, methods for producing arachidonic acid in a plant are described and uses of oils and seeds obtained from such transformed plants in a variety of food and feed applications are described.

Abstract translation: 已经转化生产花生四烯酸的油籽植物，用于这种转化的重组构建体，植物中花生四烯酸的生产方法，并描述了在各种食品和饲料应用中从这些转化植物获得的油和种子的用途。

公开(公告)号：US07588931B2

公开(公告)日：2009-09-15

申请号：US11264784

申请日：2005-11-01

Applicant: Howard Glenn Damude ,  Peter John Gillies ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  Dana M. Walters Pollak ,  James John Ragghianti ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Peter John Gillies ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  Dana M. Walters Pollak ,  James John Ragghianti ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

IPC: C12N1/16 ,  C12N9/02 ,  C12P7/64 ,  C07H21/04

CPC classification number: C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/746 ,  C12N15/815 ,  C12P7/6472 ,  A23V2250/187 ,  A23V2200/30

Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 10% arachidonic acid (ARA, an ω-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of ARA. Production host cells are claimed, as are methods for producing ARA within said host cells.

Abstract translation: 描述了能够在总油馏分中产生大于10％花生四烯酸（ARA，ω-6多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。 这些菌株包含表达异源去饱和酶，延伸酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使ARA能够合成和高度积累。 要求生产宿主细胞，以及在所述宿主细胞内产生ARA的方法。

公开(公告)号：US20090119795A1

公开(公告)日：2009-05-07

申请号：US11992899

申请日：2006-11-16

Applicant: Howard Glenn Damude ,  Brian McGonigle ,  Quinn Qun Zhu ,  Zhixiong Xue

Inventor： Howard Glenn Damude ,  Brian McGonigle ,  Quinn Qun Zhu ,  Zhixiong Xue

IPC: A01H1/00 ,  C07H21/04 ,  C12N9/10 ,  A01H5/10 ,  C12P7/64 ,  C12N5/14 ,  C12N15/82

CPC classification number: C12N15/8247 ,  C11B1/025 ,  C12N9/1029 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.

Abstract translation: 分离的核酸片段和包含编码Δ-9延伸酶的这种片段的重组构建物以及在植物中使用这些δ-9延长酶制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US08927809B2

公开(公告)日：2015-01-06

申请号：US13295345

申请日：2011-11-14

Applicant: Knut Meyer ,  William D. Hitz ,  Narendra S. Yadav ,  Howard Glenn Damude

Inventor： Knut Meyer ,  William D. Hitz ,  Narendra S. Yadav ,  Howard Glenn Damude

IPC: A01H5/00 ,  A01H4/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/54 ,  C12P21/00 ,  C12P7/64 ,  A23L1/20

CPC classification number: C12N15/8247 ,  C12N9/1029 ,  C12Y203/01158

Abstract: Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from Yarrowia Lipolytica are used to achieve the increase in seed storage lipids.

Abstract translation: 描述了与非转基因，无偏分离大豆种子的总脂肪酸含量相比，具有至少10％的总脂肪酸含量增加和改变的脂肪酸分布的转基因大豆种子。 来自解脂耶氏酵母的DGAT基因用于实现种子储存脂质的增加。

公开(公告)号：US20130006004A1

公开(公告)日：2013-01-03

申请号：US13611554

申请日：2012-09-12

Applicant: Howard Glenn Damude ,  Narendra S. Yadav

Inventor： Howard Glenn Damude ,  Narendra S. Yadav

IPC: C11B1/00 ,  A23D9/02 ,  C07F9/10

CPC classification number: C12N9/0083 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472

Abstract: The present invention relates to fungal Δ-15 fatty acid desaturases that are able to catalyze the conversion of linoleic acid (18:2, LA) to alpha-linolenic acid (18:3, ALA). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host plants and microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific omega-3 and omega-6 fatty acids by over-expression of the Δ-15 fatty acid desaturases are also described herein.

Abstract translation: 本发明涉及能够催化亚油酸（18:2，LA）转化为α-亚麻酸（18:3，ALA）的真菌和Dgr-15脂肪酸去饱和酶。 描述了编码去饱和酶的核酸序列，与其杂交的核酸序列，包含去饱和酶基因的DNA构建体和表达增加的去饱和酶水平的重组宿主植物和微生物。 本文还描述了通过过量表达＆Dgr-15脂肪酸去饱和酶来增加特定ω-3和ω-6脂肪酸的产生的方法。

公开(公告)号：US08298797B2

公开(公告)日：2012-10-30

申请号：US12825523

申请日：2010-06-29

Applicant: Howard Glenn Damude ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Quinn Qun Zhu

IPC: C12P7/66 ,  C12N15/74 ,  A01N63/00 ,  C07H21/04 ,  A61K48/00

CPC classification number: C12N9/1029 ,  A23D9/00 ,  A23D9/007 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  A23V2250/1862 ,  A23V2250/187 ,  A23V2250/1868

Abstract: The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid (LA; 18:2 ω-6) to eicosadienoic acid (EDA; 20:2 ω-6) and/or α-linolenic acid (ALA; 18:3 ω-3) to eicosatrienoic acid (ETrA; 20:3 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in oleaginous yeast are disclosed.

Abstract translation: 本发明涉及具有将亚油酸（LA; 18：2ω-6）转化成二十碳烯酸（EDA;20:2ω-6）和/或α-亚麻酸（ALA）的能力的Dgr。 ; 18：3ω-3）至二十碳三烯酸（ETrA; 20：3ω-3）。 公开了分离的核酸片段和重组构建体，其包含编码'Dgr。9延伸酶的这种片段以及在含油酵母中使用这些＆Dgr.9延伸酶制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20110268861A1

公开(公告)日：2011-11-03

申请号：US13182876

申请日：2011-07-14

Applicant: Howard Glenn DAMUDE ,  Anthony J. Kinney

Inventor： Howard Glenn DAMUDE ,  Anthony J. Kinney

IPC: C08G63/48 ,  A23D9/00 ,  A23L1/31 ,  A23C20/00 ,  A23D7/00 ,  A23K1/00 ,  A23L2/52 ,  A23C9/20 ,  A23L1/30 ,  C07C53/126 ,  A23C9/152

CPC classification number: C12N15/8247 ,  A23D9/00 ,  A23K10/30 ,  A23K20/163 ,  A23K50/80 ,  A23L33/12 ,  C12N9/0083 ,  C12N9/1029 ,  Y02A40/818

Abstract: Oilseed plants which have been transformed to produce arachidonic acid, recombinant constructs used in such transformations, methods for producing arachidonic acid in a plant are described and uses of oils and seeds obtained from such transformed plants in a variety of food and feed applications are described.

Abstract translation: 已经转化生产花生四烯酸的油籽植物，用于这种转化的重组构建体，植物中花生四烯酸的生产方法，并描述了在各种食品和饲料应用中从这些转化植物获得的油和种子的用途。

公开(公告)号：US08048653B2

公开(公告)日：2011-11-01

申请号：US12619706

申请日：2009-11-17

Applicant: Howard Glenn Damude ,  Brian McGonigle ,  Zhixiong Xue ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Brian McGonigle ,  Zhixiong Xue ,  Quinn Qun Zhu

IPC: C12P7/64 ,  C12N9/00 ,  C12N9/10 ,  C12N1/20 ,  C12N15/00

CPC classification number: C12N15/8247 ,  C11B1/025 ,  C12N9/1029 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472

Abstract: The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in plants and oleaginous yeast are disclosed.

Abstract translation: 本发明涉及具有将亚油酸[18：2，LA]转化成二十碳烯酸[20：2，EDA]的能力的“Dgr”9延伸酶。 公开了分离的核酸片段和重组构建体，其包含编码'Dgr 9延长酶的这种片段以及使用这些植物和油性酵母中的这些Dgr 9延伸酶制备长链多不饱和脂肪酸（PUFA）的方法。