公开(公告)号：US06596532B1

公开(公告)日：2003-07-22

申请号：US09502324

申请日：2000-02-11

Applicant: Jones M. Hyman ,  Paul M. Matsumura ,  Scott R. Jeffrey ,  Martin J. Maresch ,  Thurman C. Thorpe

Inventor： Jones M. Hyman ,  Paul M. Matsumura ,  Scott R. Jeffrey ,  Martin J. Maresch ,  Thurman C. Thorpe

IPC: C12M134

CPC classification number: C12M25/14 ,  C12Q1/04 ,  C12Q1/045 ,  C12Q1/24

Abstract: A device and method are provided for isolating and culturing microorganisms from a bulk fluid sample. The device comprises a container having therein a polymeric immobilization layer having interstitial spaces between polymer chains such as a gel matrix. The interstitial spaces are of an average size less than an average size of microorganisms to be separated from the sample and cultured. A bulk fluid sample is applied to the immobilization layer where fluid is absorbed by the layer and microorganisms remain on the surface of the layer. After culturing, microorganism colonies are readily accessible on the surface of the layer for harvest and testing. The immobilization layer may contain one or more of nutrients for microorganism growth, lytic agents, lytic enzymes, antibiotics, antibiotic neutralizers, indicators, detergents and selective agents. An adjacent support layer may be above and/or below the immobilization layer. The immobilization layer may be in combination with a sensor layer that changes color in areas corresponding to portions of the layer having microorganisms thereon. A membrane may be embedded in the immobilization layer for enhancing microorganism visibility and facilitating microorganism harvest.

Abstract translation: 提供了用于从大量流体样品中分离和培养微生物的装置和方法。 该装置包括其中具有聚合物固定化层的容器，其在聚合物链之间具有间隙，例如凝胶基质。 间隙空间的平均尺寸小于从样品中分离的微生物的平均尺寸并进行培养。 将大量流体样品施加到固定层，其中流体被层吸收，微生物保留在层的表面上。 培养后，微生物菌落易于在层的表面进行收获和测试。 固定层可以含有一种或多种用于微生物生长的营养物，溶解剂，溶解酶，抗生素，抗生素中和剂，指示剂，洗涤剂和选择剂。 相邻的支撑层可以在固定层的上方和/或下方。 固定层可以与在其上具有微生物层的部分相对应的区域中改变颜色的传感器层组合。 膜可以嵌入在固定层中以增强微生物的可见性并促进微生物的收获。

公开(公告)号：US06416969B2

公开(公告)日：2002-07-09

申请号：US09843202

申请日：2001-04-26

Applicant: Paul M. Matsumura ,  Jones M. Hyman ,  Scott R. Jeffrey ,  Martin J. Maresch ,  Thurman C. Thorpe ,  William G. Barron

Inventor： Paul M. Matsumura ,  Jones M. Hyman ,  Scott R. Jeffrey ,  Martin J. Maresch ,  Thurman C. Thorpe ,  William G. Barron

IPC: C12Q118

CPC classification number: C12M41/46 ,  C12M41/36 ,  C12Q1/04 ,  C12Q1/18 ,  G01N21/253 ,  G01N2035/0425

Abstract: Disposable plates for performing microbial antibiotic susceptibility testing with multiple channels can be inoculated with a microorganism and antimicrobial agent. The antimicrobial agent may be presented as a gradient of concentrations in the plate. The susceptibility testing plates are configured to allow viewing of microbial growth, and/or to be received in an automated instrument which analyzes same.

Abstract translation: 用多个通道进行微生物抗生素敏感性试验的一次性板可以用微生物和抗微生物剂接种。 抗微生物剂可以以板中的浓度梯度表示。 敏感性测试板被配置为允许观察微生物生长，和/或被接收在分析其的自动化仪器中。

公开(公告)号：US06197577B1

公开(公告)日：2001-03-06

申请号：US09320386

申请日：1999-05-27

Applicant: Scott R. Jeffrey ,  Paul M. Matsumura ,  Martin J. Maresch ,  Jones M. Hyman ,  Thurman C. Thorpe

Inventor： Scott R. Jeffrey ,  Paul M. Matsumura ,  Martin J. Maresch ,  Jones M. Hyman ,  Thurman C. Thorpe

IPC: C12M134

CPC classification number: C12Q1/04 ,  C12M23/04 ,  C12M23/22 ,  C12M23/24 ,  C12M25/06 ,  C12M25/14 ,  C12Q1/045 ,  C12Q1/24

Abstract: A device and method allow for detecting the presence of microorganisms in clinical and non-clinical specimens. The device, a sensor, provides an environment to culture microbial organism colonies from a fluid sample, and a means to facilitate microbial detection and quantification, either manually or with an instrument. The sensor has a microorganism immobilization matrix layer and a sensor layer. Detected microbial colonies are immediately available for further testing. The sensor provides an area for accepting a fluid sample, a mechanism to immobilize the fluid sample on an interior surface of the plate, nutrients to facilitate growth of microorganisms in the sample, and a sensor for allowing the detection and/or enumeration of microorganism colonies within the sample.

Abstract translation: 一种装置和方法允许检测临床和非临床标本中微生物的存在。 该装置，传感器，提供了从流体样品培养微生物菌落的环境，以及手动或用仪器促进微生物检测和定量的手段。 传感器具有微生物固定基质层和传感器层。 检测到的微生物菌落立即可用于进一步测试。 传感器提供用于接受流体样品的区域，将流体样品固定在板的内表面上的机制，营养物质以促进样品中微生物的生长，以及用于允许微生物菌落的检测和/或计数的传感器 在样品内。

公开(公告)号：US6153400A

公开(公告)日：2000-11-28

申请号：US267863

申请日：1999-03-12

Applicant: Paul M. Matsumura ,  Jones M. Hyman ,  Scott R. Jeffrey ,  Martin J. Maresch ,  Thurman C. Thorpe ,  William G. Barron

Inventor： Paul M. Matsumura ,  Jones M. Hyman ,  Scott R. Jeffrey ,  Martin J. Maresch ,  Thurman C. Thorpe ,  William G. Barron

IPC: C12M1/34 ,  C12Q1/18 ,  C12Q1/20 ,  C12R1/01 ,  G01N21/25 ,  C12M1/00 ,  C12M1/22 ,  C12Q1/04

CPC classification number: G01N21/253 ,  C12Q1/18

Abstract: A method and apparatus for performing microbial antibiotic susceptibility testing include disposable, multi-chambered susceptibility plates and an automated plate handler and image acquisition and processing instrument. The susceptibility plates are inoculated with a microorganism (any suitable organism such as bacteria, fungi, protozoa, algae or viruses) and anti-microbial agent(s) are applied such that the microorganism is exposed to a variety of concentrations, or a gradient of each anti-microbial agent. The plates are then placed in the instrument, which monitors and measures the growth (or lack thereof) of the microorganisms. This data is used to determine the susceptibility of the microorganism to the antibiotics. Such a system automates antimicrobial susceptibility testing using solid media and Kirby-Bauer standardized result reporting. The system provides a level of automation previously associated only with broth microdilution testing, while retaining the advantages of the manual disk diffusion test.

Abstract translation: 用于进行微生物抗生素敏感性试验的方法和装置包括一次性多室敏感性板和自动板处理器和图像采集和处理仪器。 用微生物（诸如细菌，真菌，原生动物，藻类或病毒的任何合适的生物体）接种敏感性板，并施用抗微生物剂，使得微生物暴露于多种浓度或梯度 每种抗微生物剂。 然后将板放置在仪器中，其监测和测量微生物的生长（或不存在）。 该数据用于确定微生物对抗生素的敏感性。 这种系统使用固体培养基和Kirby-Bauer标准化结果报告自动化抗微生物药敏试验。 该系统提供了以前仅与肉汤微量稀释测试相关联的自动化水平，同时保留了手动盘扩散测试的优点。

公开(公告)号：US2448402A

公开(公告)日：1948-08-31

申请号：US57510345

申请日：1945-01-29

Applicant: BRIAN JONES M

Inventor： THOMPSON BOYD S

IPC: H01R11/15

CPC classification number: H01R11/15

公开(公告)号：US10774300B2

公开(公告)日：2020-09-15

申请号：US13554704

申请日：2012-07-20

Applicant: Jones M. Hyman ,  John D. Walsh ,  Ronnie J. Robinson ,  Christopher Ronsick

Inventor： Jones M. Hyman ,  John D. Walsh ,  Ronnie J. Robinson ,  Christopher Ronsick

IPC: C12Q1/689 ,  C12Q1/6888 ,  C12M1/00 ,  C12Q1/04 ,  C12N1/02 ,  C12N1/06 ,  C12Q1/6895 ,  C12Q1/24

Abstract: The present invention is directed to methods and kits for separating, accumulating, characterizing and/or identifying microorganisms known to be present or that may be present in a test sample. The method of the invention comprises optional lysing non-microorganism cells and/or particulates that may be present in a test sample, followed by a subsequent filtration step for isolation and/or accumulation of microorganisms. The kit of the present invention may comprise at least one filter membrane or an integrated filtration and sample transfer device for isolation and/or accumulation of microorganisms.

公开(公告)号：US09822389B2

公开(公告)日：2017-11-21

申请号：US14444708

申请日：2014-07-28

Applicant: Jones M. Hyman ,  John D. Walsh ,  Thurman C. Thorpe

Inventor： Jones M. Hyman ,  John D. Walsh ,  Thurman C. Thorpe

IPC: C12Q1/04

CPC classification number: C12Q1/04

Abstract: The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.

公开(公告)号：US09128058B2

公开(公告)日：2015-09-08

申请号：US12589985

申请日：2009-10-30

Applicant: John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

Inventor： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC: C12Q1/04 ,  G01N21/65 ,  G01N33/68 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64

CPC classification number: C12Q1/24 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/6816 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

Abstract: The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for the use of one or more identifier agents and interrogating the microorganism sample and/or said one or more identifier agents to produce measurements which characterizing and/or identifying the microorganism based on the produced measurements and/or the presence or absence of the identifier agent or a metabolized form of the identifier agent in the microorganism sample.

Abstract translation: 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明进一步提供一种或多种识别剂的使用，并且询问微生物样品和/或所述一种或多种标识物以产生基于产生的测量值和/或存在或不存在来表征和/或鉴定微生物的测量结果 的识别剂或微生物样品中识别剂的代谢形式。

公开(公告)号：US08841118B2

公开(公告)日：2014-09-23

申请号：US12800467

申请日：2010-05-14

Applicant: Ronnie J. Robinson ,  Mark S. Wilson ,  Christopher S. Ronsick ,  John D. Walsh ,  Jones M. Hyman ,  Thurman C. Thorpe ,  Bradford G. Clay

Inventor： Ronnie J. Robinson ,  Mark S. Wilson ,  Christopher S. Ronsick ,  John D. Walsh ,  Jones M. Hyman ,  Thurman C. Thorpe ,  Bradford G. Clay

IPC: C12M1/00 ,  C12M3/00 ,  G01N35/00 ,  C12M1/34 ,  C12Q1/04 ,  G01N35/04

CPC classification number: C12Q1/04 ,  C12M41/36 ,  C12Q1/02 ,  G01N35/00603 ,  G01N35/0099 ,  G01N35/026 ,  G01N2035/00148 ,  G01N2035/0406 ,  G01N2035/0453 ,  G01N2035/0465

Abstract: A detection instrument determines whether a specimen container (e.g., blood culture bottle) is positive for presence of microbial agent growth therein. When the container is deemed positive it is made available (e.g, transferred or exposed to) to an automated instrument performing identification and/or characterization of the microbial agent. The identification and/or characterization instrument removes a portion of the sample from the specimen container and places it into a disposable separation and concentration device. The microbial agent is concentrated via optional selective lysis of non-microbial agent cellular material which may be present and centrifugation. A reading module reads the concentrated microbial agent using spectroscopic methods, e.g., measurements of intrinsic fluorescence. Such interrogation may occur while the microbial agent remains concentrated in the disposable device.

Abstract translation: 检测仪器确定样品容器（例如血培养瓶）是否对其中存在微生物剂生长是阳性的。 当容器被认为是正的时，它被提供（例如，转移或暴露于）到执行微生物剂的鉴定和/或表征的自动化仪器。 识别和/或表征仪器将样品的一部分从样品容器中取出并将其放入一次性分离和浓缩装置中。 微生物剂通过选择性裂解可能存在的和非离子的非微生物剂细胞材料而浓缩。 阅读模块使用光谱方法读取浓缩的微生物剂，例如固有荧光的测量。 当微生物剂保持集中在一次性装置中时，可能发生这种询问。

公开(公告)号：US20130045532A1

公开(公告)日：2013-02-21

申请号：US13554704

申请日：2012-07-20

Applicant: Jones M. Hyman ,  John D. Walsh ,  Ronnie J. Robinson ,  Christopher Ronsick

Inventor： Jones M. Hyman ,  John D. Walsh ,  Ronnie J. Robinson ,  Christopher Ronsick

IPC: C12M1/26 ,  C12M1/40

CPC classification number: C12M47/02 ,  C12N1/02 ,  C12N1/06 ,  C12Q1/24

Abstract: The present invention is directed to methods and kits for separating, accumulating, characterizing and/or identifying microorganisms known to be present or that may be present in a test sample. The method of the invention comprises optional lysing non-microorganism cells and/or particulates that may be present in a test sample, followed by a subsequent filtration step for isolation and/or accumulation of microorganisms. The kit of the present invention may comprise at least one filter membrane or an integrated filtration and sample transfer device for isolation and/or accumulation of microorganisms.

Abstract translation: 本发明涉及用于分离，积累，表征和/或鉴定已知存在或可能存在于测试样品中的微生物的方法和试剂盒。 本发明的方法包括任选地裂解可能存在于测试样品中的非微生物细胞和/或颗粒，随后进行用于分离和/或积累微生物的随后的过滤步骤。 本发明的试剂盒可以包括用于分离和/或积累微生物的至少一个过滤膜或一体化过滤和样品转移装置。