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公开(公告)号：US08003330B2
公开(公告)日：2011-08-23
申请号：US12286119
申请日：2008-09-26
Applicant: Cheryl Heiner , Stephen Turner , Kevin Travers
Inventor： Cheryl Heiner , Stephen Turner , Kevin Travers
CPC classification number: C12Q1/6806 , C12Q1/6869 , C12Q2563/179 , C12Q2563/155 , C12Q2525/307
Abstract: Provided are methods of producing low copy number circularized nucleic acid variants that can be distributed to reaction volumes. The methods include providing a template nucleic acid; producing a population of clonal nucleic acids from the template nucleic acid; generating a set of partially overlapping nucleic acid fragments from the population of clonal nucleic acids; circularizing the partially overlapping nucleic acid fragments to produce circularized nucleic acid variants; and aliquotting the circularized nucleic acid variants into reaction volumes. Related compositions of nucleic acid templates are also provided.
Abstract translation: 提供了可以分配到反应体积的低拷贝数环状核酸变体的生产方法。 所述方法包括提供模板核酸; 从模板核酸产生克隆核酸群; 从克隆核酸群体产生一组部分重叠的核酸片段; 使部分重叠的核酸片段环化以产生环化的核酸变体; 并将环化的核酸变体等分成反应体积。 还提供了核酸模板的相关组合物。
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公开(公告)号：US09175341B2
公开(公告)日：2015-11-03
申请号：US12635618
申请日：2009-12-10
Applicant: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
Inventor： Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
IPC: C12Q1/68
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
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公开(公告)号：US08658364B2
公开(公告)日：2014-02-25
申请号：US13427725
申请日：2012-03-22
Applicant: Thang Pham , Arunashree Bhamidipati , Kevin Travers , Eric Olivares , Tyson A. Clark , Jonas Korlach
Inventor： Thang Pham , Arunashree Bhamidipati , Kevin Travers , Eric Olivares , Tyson A. Clark , Jonas Korlach
CPC classification number: C12Q1/6874 , B01L9/523 , C12Q1/6869 , C40B60/14 , G01N33/54313 , G01N33/54353 , G01N2333/922 , C12Q2521/543 , C12Q2523/303 , C12Q2525/301
Abstract: Compositions, methods and systems are provided for the isolation of polymerase-nucleic acid complexes. Complexes can be separated from free enzyme by using hook molecules to target single stranded regions on the nucleic acid. Active complexes can be isolated from mixtures having both active and inactive complexes by initiating nucleic acid synthesis so as to open up a portion of a double stranded region rendering that region single stranded. Hook molecules are targeted to bind the sequences that are thus exposed. The hook molecules bound to active polymerase-nucleic acid complex are isolated, and the active polymerase-nucleic acid complexes released.
Abstract translation: 提供了用于分离聚合酶 - 核酸复合物的组合物，方法和系统。 通过使用钩分子将复合物与游离酶分离以靶向核酸上的单链区域。 通过引发核酸合成，可以从具有活性和非活性复合物的混合物中分离活性配合物，以便打开一部分双链区域，使该区域成为单链。 钩状分子被靶向以结合由此暴露的序列。 分离与活性聚合酶 - 核酸复合物结合的钩分子，释放活性聚合酶 - 核酸复合物。
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公开(公告)号：US08535886B2
公开(公告)日：2013-09-17
申请号：US13494729
申请日：2012-06-12
Applicant: Pranav Patel , Keith Bjornson , Kevin Travers , Cheryl Heiner
Inventor： Pranav Patel , Keith Bjornson , Kevin Travers , Cheryl Heiner
Abstract: Provided are methods and compositions for the production of linear single-stranded nucleic acids, which can be used as templates in high-throughput sequencing systems. Also provided are methods and compositions for the production of closed single-stranded nucleic acid loops, which can be used as templates in high-throughput sequencing systems.
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公开(公告)号：US08455193B2
公开(公告)日：2013-06-04
申请号：US13403789
申请日：2012-02-23
Applicant: Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
Inventor： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC classification number: G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
Abstract: Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.
Abstract translation: 用于核酸测序的组合物和方法包括在部分或完全邻接的构建体中包含双链部分的模板构建体，以通过测序有义链和反义链中的一个或两个进行冗余序列测定，并对整个构建体进行多次迭代测序。 额外的序列成分也可选地包括在这样的模板构建体内。 还提供了这些构建体的使用和制备方法以及其应用的测序组合物。
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Patent Agency Ranking
公开(公告)号：US20130029853A1
公开(公告)日：2013-01-31
申请号：US13633673
申请日：2012-10-02
Applicant: Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
Inventor： Benjamin Flusberg , Stephen Turner , Jessica Lee , Lei Jia , Jonas Korlach , Jon Sorenson , Dale Webster , John Lyle , Kevin Travers , Jeremiah Hanes , Joseph Puglisi
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q2561/113 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
Abstract translation: 提供了方法，组合物和系统用于表征修饰的核酸。 在某些优选实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基，酶修饰的碱基，脱碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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公开(公告)号：US08309330B2
公开(公告)日：2012-11-13
申请号：US13019220
申请日：2011-02-01
Applicant: Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
Inventor： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC classification number: G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
Abstract: Methods, substrates, and systems for diagnostic sequencing are provided. Small circles of nucleic acids from about 10 bases to about 200 bases can be sequenced, for example using template dependent sequencing by synthesis. The use of small circles of nucleic acids allows for repeated sequencing of the same portions of the nucleic acid, providing for higher accuracy sequence determinations.
Abstract translation: 提供了用于诊断测序的方法，底物和系统。 可以对约10个碱基至约200个碱基的小核酸核酸进行测序，例如使用通过合成进行模板依赖性测序。 核酸的小圈的使用允许对核酸的相同部分进行重复测序，提供更高精度的序列测定。
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公开(公告)号：US20120322666A1
公开(公告)日：2012-12-20
申请号：US13427725
申请日：2012-03-22
Applicant: Thang Pham , Arunashree Bhamidipati , Kevin Travers , Eric Olivares , Tyson A. Clark , Jonas Korlach
Inventor： Thang Pham , Arunashree Bhamidipati , Kevin Travers , Eric Olivares , Tyson A. Clark , Jonas Korlach
CPC classification number: C12Q1/6874 , B01L9/523 , C12Q1/6869 , C40B60/14 , G01N33/54313 , G01N33/54353 , G01N2333/922 , C12Q2521/543 , C12Q2523/303 , C12Q2525/301
Abstract: Compositions, methods and systems are provided for the isolation of polymerase-nucleic acid complexes. Complexes can be separated from free enzyme by using hook molecules to target single stranded regions on the nucleic acid. Active complexes can be isolated from mixtures having both active and inactive complexes by initiating nucleic acid synthesis so as to open up a portion of a double stranded region rendering that region single stranded. Hook molecules are targeted to bind the sequences that are thus exposed. The hook molecules bound to active polymerase-nucleic acid complex are isolated, and the active polymerase-nucleic acid complexes released.
Abstract translation: 提供了用于分离聚合酶 - 核酸复合物的组合物，方法和系统。 通过使用钩分子将复合物与游离酶分离以靶向核酸上的单链区域。 通过引发核酸合成，可以从具有活性和非活性复合物的混合物中分离活性配合物，以便打开一部分双链区域，使该区域成为单链。 钩状分子被靶向以结合由此暴露的序列。 分离与活性聚合酶 - 核酸复合物结合的钩分子，释放活性聚合酶 - 核酸复合物。
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公开(公告)号：US20110281768A1
公开(公告)日：2011-11-17
申请号：US13019220
申请日：2011-02-01
Applicant: Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
Inventor： Kevin Travers , Geoff Otto , Stephen Turner , Cheryl Heiner , Congcong Ma
CPC classification number: G06F19/22 , C12Q1/6806 , C12Q1/6855 , C12Q1/6869 , C12Q1/689 , C12Q2525/307 , C12Q2525/301 , C12Q2525/155 , C12Q2531/125 , C12Q2535/122
Abstract: Methods, substrates, and systems for diagnostic sequencing are provided. Small circles of nucleic acids from about 10 bases to about 200 bases can be sequenced, for example using template dependent sequencing by synthesis. The use of small circles of nucleic acids allows for repeated sequencing of the same portions of the nucleic acid, providing for higher accuracy sequence determinations.
Abstract translation: 提供了用于诊断测序的方法，底物和系统。 可以对约10个碱基至约200个碱基的小核酸核酸进行测序，例如使用通过合成进行模板依赖性测序。 核酸的小圈的使用允许对核酸的相同部分进行重复测序，提供更高精度的序列测定。
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公开(公告)号：US20110183320A1
公开(公告)日：2011-07-28
申请号：US12945767
申请日：2010-11-12
Applicant: Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
Inventor： Benjamin Flusberg , Jonas Korlach , Jeffrey Wegener , Tyson A. Clark , Igor Vilfan , Andrey Kislyuk , Stephen Turner , Jon Sorenson , Kevin Travers , Cheryl Heiner , Austin B. Tomaney , Patrick Marks , Jessica Lee , Lei Jia , Dale Webster , John Lyle , Jeremiah Hanes , Joseph Puglisi
IPC: C12Q1/68
CPC classification number: C12Q1/6869 , C12Q1/6837 , C12Q1/6858 , C12Q1/6874 , C12Q1/689 , C12Q2600/154 , C12Q2537/164 , C12Q2527/113 , C12Q2521/101 , C12Q2525/307 , C12Q2525/117
Abstract: Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.
Abstract translation: 提供了方法，组合物和系统用于表征修饰的核酸。 在某些优选实施方案中，提供单分子测序方法用于鉴定核酸序列内的修饰的核苷酸。 通过本文提供的方法可检测的修饰物包括化学修饰的碱基，酶修饰的碱基，脱碱基位点，非天然碱基，二级结构和与模板核酸结合的试剂。
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