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    Preparation of tissue equivalents by contraction of a collagen gel layered on a collagen gel
    11.
    发明授权
    Preparation of tissue equivalents by contraction of a collagen gel layered on a collagen gel 失效
    通过层叠在胶原凝胶上的胶原凝胶的收缩来制备组织当量

    公开(公告)号：US5536656A

    公开(公告)日：1996-07-16

    申请号：US193809

    申请日：1994-02-09

    申请人： Paul Kemp ,  Eugene Bell ,  David T. Kagan ,  Valerie Mason ,  John Cavallaro

    发明人： Paul Kemp ,  Eugene Bell ,  David T. Kagan ,  Valerie Mason ,  John Cavallaro

    IPC分类号： A61L27/00 ,  A61L27/60 ,  C12M3/06 ,  C12N5/071 ,  G01N33/50 ,  C12N5/00 ,  A61F2/00 ,  A61F13/00 ,  C12M3/04

    CPC分类号： C12N5/0698 ,  A61L27/60 ,  C12M25/04 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2533/54

    摘要： Tissue equivalents are produced by applying a solution of collagen without cells to a permeable membrane, gelling to produce a collagen gel on the membrane, applying a mixture of collagen and a contractile agent to the collagen gel, gelling the mixture and allowing the resultant gel to undergo radial contraction which is controlled by the collagen gel on the membrane. A suitable contractile agent is fibroblast cells. A skin tissue equivalent is produced by disposing human epidermal cells on the contracted collagen gel and allowing epidermalization to occur. A nutrient medium can be supplied to the cells. An absorbent member may be disposed adjacent the permeable membrane opposite the collagen gel on the membrane. The permeable membrane can be the bottom of an inner well disposed in an outer well for containing a nutrient medium in contact with the membrane. The nutrient medium may be contained in the outer well in an agarose gel in contact with the membrane.

    摘要翻译： 组织当量是通过将无细胞的胶原溶液施用于可渗透的膜，凝胶化以在膜上产生胶原凝胶，将胶原蛋白和收缩剂的混合物施用于胶原凝胶，胶凝该混合物并使所得凝胶 经受由膜上的胶原凝胶控制的径向收缩。 合适的收缩剂是成纤维细胞。 通过将人表皮细胞置于收缩的胶原凝胶上并允许发生表皮化而产生皮肤组织当量。 可以向细胞提供营养培养基。 吸收构件可以设置在与膜上的胶原凝胶相对的可渗透膜附近。 渗透膜可以是设置在外壁中的用于容纳与膜接触的营养培养基的内部井的底部。 营养培养基可以包含在与膜接触的琼脂糖凝胶的外孔中。

    Data formater/converter for use with solid-state disk memory using storage devices with defects
    12.
    发明授权
    Data formater/converter for use with solid-state disk memory using storage devices with defects 失效

    公开(公告)号：US5343426A

    公开(公告)日：1994-08-30

    申请号：US897164

    申请日：1992-06-11

    申请人： Charles Cassidy ,  Paul Kemp

    发明人： Charles Cassidy ,  Paul Kemp

    IPC分类号： G06F11/10 ,  G11C29/00 ,  H03M13/00

    CPC分类号： G06F11/1044 ,  G11C29/88

    摘要： A computer system includes a main memory that is able to make use of DRAM memory devices having a relatively high level of bad cells (hard faults). An EDC circuit is provided which uses combinatorial logic to perform a BCH code type of error detection and correction. A primary feature is the recognition that due to use of high density integrated circuits--gate arrays--it is no longer necessary to use sequential logic to decode the multiple-bit error correcting codes. An EDC with 128-bits of data and a check bit field 41-bits wide, using a BCH code, constructed in ASIC sea-of-gates technology using about 87,000 logic gates, can correct 5-bits in error and can detect 6-bits in error. By using multiple-bit EDC in the controller for main memory, it is no longer necessary that all DRAM devices be ostensibly "perfect." A certain density of non-functional memory cells can be tolerated, yet the memory system will still return perfect data. The added cost of multiple-bit EDC, including the added cost of extra storage for the check bits and the EDC circuit itself, is more than compensated by reduced cost of the DRAMs. In a addition the computer system includes a solid-state disk type memory for a computer system is able to make use of DRAM memory devices having a relatively high level of bad cells (hard faults). An EDC circuit is employed to perform a Reed-Solomon code type of error detection and correction. A primary feature is the recognition that it is no longer necessary that all DRAM devices be ostensibly "perfect." A certain density of non-functional memory cells can be tolerated, yet the memory system will still return perfect data. The added cost of multiple-bit EDC, including the added cost of extra storage for the check bits and the EDC circuit itself, is more than compensated by reduced cost of the DRAMs. A preferred data formatter circuit to convert between symbol and word data is also described.

    Skin Equivalent Culture
    13.
    发明申请
    Skin Equivalent Culture 有权
    皮肤等效文化

    公开(公告)号：US20100203135A1

    公开(公告)日：2010-08-12

    申请号：US11886113

    申请日：2006-03-14

    申请人： Paul Kemp ,  David Shering ,  Andrew Shering ,  Penny Johnson ,  Damian Marshall

    发明人： Paul Kemp ,  David Shering ,  Andrew Shering ,  Penny Johnson ,  Damian Marshall

    IPC分类号： A61K9/14 ,  C07K14/78 ,  A61K38/39 ,  C07D233/74 ,  C07D307/62 ,  C07C53/128 ,  C07K7/64 ,  C07D213/80 ,  C07D281/10 ,  C07C255/43 ,  A61P17/02 ,  C12P21/02

    CPC分类号： C12N5/0698 ,  A61L27/20 ,  A61L27/225 ,  A61L27/24 ,  A61L27/3687 ,  A61L27/3691 ,  A61L27/3804 ,  A61L27/3839 ,  A61L27/3886 ,  A61L27/3895 ,  A61L27/54 ,  A61L27/60 ,  C12N2500/25 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2501/11 ,  C12N2501/15 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2533/56

    摘要： Methods of forming soft connective tissue compositions such as skin equivalents, compositions made by the methods and their uses. In particular, a method of forming a connective tissue equivalent, comprising the steps of: (i) incubating collagen-producing cells in or on a support matrix; (ii) inducing and/or enhancing collagen production by the collagen-producing cells to form a collagenous construct and degradation and replacement of the support matrix; (iii) freeze-drying the construct; and (iv) re-populating the freeze-dried construct with collagen-producing cells and/or epithelial cells and/or endothelial cells and/or mesenchymal cells, thereby forming a connective tissue equivalent, wherein: (a) the collagen-producing cells are substantially fibroblasts; for example human neonatal dermal fibroblasts; (b) the support matrix is a provisional support matrix in which the support matrix is a fibrin matrix, for example formed by thrombin-mediated polymerisation of fibrinogen; and (c) as a result of the collagen production by the collagen-producing cells the provisional fibrin support matrix is digested by the cells and is replaced by collagen, thereby essentially replacing the provisional fibrin matrix with a collagen matrix synthesised in situ by the cells.

    摘要翻译： 形成软结缔组织组合物如皮肤当量的方法，通过方法制备的组合物及其用途。 特别地，形成结缔组织当量的方法，包括以下步骤：（i）在载体基质中或载体上孵育胶原产生细胞; （ii）诱导和/或增强胶原产生细胞的胶原产生以形成胶原构建物并降解和置换支持基质; （iii）冷冻干燥该构造体; 和（iv）用胶原产生细胞和/或上皮细胞和/或内皮细胞和/或间充质细胞重新填充冷冻干燥的构建体，从而形成结缔组织当量，其中：（a）胶原产生细胞 基本上是成纤维细胞; 例如人类新生儿真皮成纤维细胞; （b）载体基质是临时支持基质，其中载体基质是纤维蛋白基质，例如通过凝血酶介导的纤维蛋白原聚合形成; 和（c）作为胶原蛋白产生细胞产生胶原的结果，临床纤维蛋白载体基质被细胞消化并被胶原替代，从而基本上用由细胞原位合成的胶原基质代替临时纤维蛋白基质 。

    Air-bearing center-guiding apparatus and method
    14.
    发明授权
    Air-bearing center-guiding apparatus and method 失效
    空气轴承中心导向装置及方法

    公开(公告)号：US4892243A

    公开(公告)日：1990-01-09

    申请号：US247224

    申请日：1988-09-21

    申请人： Michael Long ,  Thomas W. Palone ,  Paul Kemp

    发明人： Michael Long ,  Thomas W. Palone ,  Paul Kemp

    IPC分类号： B65H20/10 ,  B65H23/02 ,  B65H23/032 ,  B65H23/24

    CPC分类号： B65H23/02 ,  B65H23/032 ,  B65H23/24 ,  B65H2406/111

    摘要： An air-bearing center-guiding apparatus 10 and a method are disclosed for supporting and laterally center-guiding thin flexible webs of paper or plastic under 15 mils in thickness. The apparatus comprises a web support and guide member 22 having an inner surface and an outer web-facing surface. Parallel rows of elongated spaced-apart guide apertures 34 extend longitudinally along edge regions of the web. Each guide aperture 34 is formed from a cylindrical opening in the web support and guide member 22 and extends substantially perpendicular to the web. Within each aperture a flat-sided dowel 60 is mounted to define a flow passage shaped in cross-section like a segment of a circle 62. Each circular-segment guide aperture 34 has a straight side 54 and a curved side 52 for directing a jet of air against the web edge 56. A plurality of the circular-segment guide apertures 34 develop a guiding force for holding lateral movement of the web to plus or minus 0.001 inch (0.0254 mm). A row of web-support apertures 32, separate from the guide apertures 34, is provided between the rows of guide apertures 34 to form an air bearing for supporting the web.

    摘要翻译： 公开了一种空气轴承中心引导装置10和方法，用于支撑和横向中心地引导厚度小于15密耳的纸或塑料的薄柔性幅材。 该装置包括具有内表面和面向外网的表面的腹板支撑件和引导构件22。 细长间隔开的引导孔34的平行排沿腹板的边缘区域纵向延伸。 每个引导孔34由幅材支撑件和引导构件22中的圆柱形开口形成并基本上垂直于幅材延伸。 在每个孔内，平面定位销60被安装以限定如同圆形部分62的横截面形状的流动通道。每个圆形段导向孔34具有直的侧面54和用于引导射流的弯曲侧面52 多个圆形段导向孔34形成用于将幅材的横向运动保持在正或负0.001英寸（0.0254mm）的引导力。 与引导孔34分开的一排腹板支撑孔32设置在引导孔34的行之间，以形成用于支撑腹板的空气轴承。

    Web center-guiding apparatus
    15.
    发明授权
    Web center-guiding apparatus 失效
    网络中心引导装置

    公开(公告)号：US4889269A

    公开(公告)日：1989-12-26

    申请号：US254341

    申请日：1988-09-21

    申请人： Michael Long ,  Thomas W. Palone ,  Paul Kemp

    发明人： Michael Long ,  Thomas W. Palone ,  Paul Kemp

    IPC分类号： B65H20/10 ,  B65H23/02 ,  B65H23/032 ,  B65H23/24

    CPC分类号： B65H23/24 ,  B65H23/02 ,  B65H23/032 ,  B65H2406/111

    摘要： An air-bearing center-guiding apparatus is disclosed for supporting and laterally center-guiding thin, flexible webs of paper or plastic under 15 mils in thickness. The apparatus comprises a web support and guide member 10 having an inner surface 58 and an outer web-facing surface 60. The web support and guide member 10 comprises a base member 14 for supporting an inner ring 22, an end cap 38, and a center ring 28 interposed therebetween. Parallel, circumferentially extending rows of circumferentially elongated and spaced-apart guide apertures 62 are provided in the web support and guide member 10 along edge regions of the web. The guide apertures 62 are formed between end surfaces 46 of the center ring 28 and interengaging end surfaces of the inner ring 22 and end cap rings 38. Each guide aperture 62 extends substantially perpendicular to the surface of the web and defines in cross-section an opening having a straight side and a curved side for directing jets of air against the web edges for developing a guiding force for holding lateral movement of the web to plus or minus 0.001 inch (0.0254 mm). A row of support apertures 56, separate from the guide apertures 62, is provided in the center ring 28 between the rows of guide apertures 62 to form an air-bearing for the web.

    摘要翻译： 公开了一种空气轴承中心引导装置，用于支撑和横向中心地引导厚度小于15密耳的薄而柔软的纸或塑料幅材。 该装置包括腹板支撑件和引导构件10，该构件具有内表面58和外腹板相对表面60.腹板支撑件和导向构件10包括用于支撑内圈22，端盖38和 中间环28插入其间。 平行的沿周向细长且间隔开的导向孔62的周向延伸的排沿腹板的边缘区域设置在腹板支撑件和引导构件10中。 引导孔62形成在中心环28的端面46和内环22和端盖环38的相互接合的端面之间。每个引导孔62基本上垂直于网的表面延伸并且在横截面中限定 具有直边和弯曲侧的开口，用于将空气的喷射引导到幅材边缘上，用于形成用于将幅材的横向移动保持在正或负0.001英寸（0.0254mm）的引导力。 在引导孔62的行之间的中心环28中设置有与引导孔62分离的一排支撑孔56，以形成腹板的空气轴承。

    Air treating gel
    16.
    发明授权
    Air treating gel 失效
    空气处理凝胶

    公开(公告)号：US3997480A

    公开(公告)日：1976-12-14

    申请号：US527168

    申请日：1974-11-25

    申请人： Alan Singleton ,  Paul Kemp Montagnat

    发明人： Alan Singleton ,  Paul Kemp Montagnat

    IPC分类号： A61L9/04 ,  A61K7/46 ,  C11B9/00

    CPC分类号： A61L9/048

    摘要： An air treating gel comprises an aqueous medium, at least one volatile air treating component and a gelling agent, characterized in that the gelling agent consists of the reaction product of a water soluble cellulose derivative and a metal salt.The invention also covers an apparatus for continuously producing the above air treating gel comprising a pair of containers holding the aqueous solution of said cellulose derivative and the slurry of said metal salt and said volatile air treating component respectively, mixing means connected to the containers for mixing and reacting the aqueous solution and slurry to form the gel, and transfer means for continuously transferring the aqueous solution and slurry in predetermined volume proportion from the containers to the mixing means.

    摘要翻译： 空气处理凝胶包括水性介质，至少一种挥发性空气处理组分和胶凝剂，其特征在于胶凝剂由水溶性纤维素衍生物和金属盐的反应产物组成。

    Skin equivalent culture
    19.
    发明授权
    Skin equivalent culture 有权
    皮肤等效文化

    公开(公告)号：US08114670B2

    公开(公告)日：2012-02-14

    申请号：US11886113

    申请日：2006-03-14

    申请人： Paul Kemp ,  David Shering ,  Andrew Shering, legal representative ,  Penny Johnson ,  Damian Marshall

    发明人： Paul Kemp ,  David Shering ,  Penny Johnson ,  Damian Marshall

    IPC分类号： C12N5/00 ,  G01N33/53 ,  A01N63/00 ,  A61K9/14

    CPC分类号： C12N5/0698 ,  A61L27/20 ,  A61L27/225 ,  A61L27/24 ,  A61L27/3687 ,  A61L27/3691 ,  A61L27/3804 ,  A61L27/3839 ,  A61L27/3886 ,  A61L27/3895 ,  A61L27/54 ,  A61L27/60 ,  C12N2500/25 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2501/11 ,  C12N2501/15 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2533/56

    摘要： Methods of forming soft connective tissue compositions such as skin equivalents, compositions made by the methods and their uses. In particular, a method of forming a connective tissue equivalent, comprising the steps of: (i) incubating collagen-producing cells in or on a support matrix; (ii) inducing and/or enhancing collagen production by the collagen-producing cells to form a collagenous construct and degradation and replacement of the support matrix; (iii) freeze-drying the construct; and (iv) re-populating the freeze-dried construct with collagen-producing cells and/or epithelial cells and/or endothelial cells and/or mesenchymal cells, thereby forming a connective tissue equivalent, wherein: (a) the collagen-producing cells are substantially fibroblasts; for example human neonatal dermal fibroblasts; (b) the support matrix is a provisional support matrix in which the support matrix is a fibrin matrix, for example formed by thrombin-mediated polymerisation of fibrinogen; and (c) as a result of the collagen production by the collagen-producing cells the provisional fibrin support matrix is digested by the cells and is replaced by collagen, thereby essentially replacing the provisional fibrin matrix with a collagen matrix synthesised in situ by the cells.

    摘要翻译： 形成软结缔组织组合物如皮肤当量的方法，通过方法制备的组合物及其用途。 特别地，形成结缔组织当量的方法，包括以下步骤：（i）在载体基质中或载体上孵育胶原产生细胞; （ii）诱导和/或增强胶原产生细胞的胶原产生以形成胶原构建物并降解和置换支持基质; （iii）冷冻干燥该构造体; 和（iv）用胶原产生细胞和/或上皮细胞和/或内皮细胞和/或间充质细胞重新填充冷冻干燥的构建体，从而形成结缔组织当量，其中：（a）胶原产生细胞 基本上是成纤维细胞; 例如人类新生儿真皮成纤维细胞; （b）载体基质是临时支持基质，其中载体基质是纤维蛋白基质，例如通过凝血酶介导的纤维蛋白原聚合形成; 和（c）作为胶原蛋白产生细胞产生胶原的结果，临床纤维蛋白载体基质被细胞消化并被胶原替代，从而基本上用由细胞原位合成的胶原基质代替临时纤维蛋白基质 。