公开(公告)号：US20120040437A1

公开(公告)日：2012-02-16

申请号：US13223604

申请日：2011-09-01

Applicant: Svetlana Balatskaya ,  Birthe Borup ,  Ranjini Chatterjee ,  Ish Dhawan ,  Richard J. Fox ,  Kenneth W. Mitchell ,  Emily C. Mundorff ,  Les Partridge ,  Matthew Tobin

Inventor： Svetlana Balatskaya ,  Birthe Borup ,  Ranjini Chatterjee ,  Ish Dhawan ,  Richard J. Fox ,  Kenneth W. Mitchell ,  Emily C. Mundorff ,  Les Partridge ,  Matthew Tobin

IPC: C12N9/90

CPC classification number: C12N9/90 ,  C12P13/06

Abstract: The present disclosure relates generally to polypeptides having improved alanine 2,3-aminomutase (AAM) activity, the polynucleotides encoding the AAM polypeptides, and expression vectors and host cells for expressing the AAM polypeptides.

Abstract translation: 本公开一般涉及具有改进的丙氨酸2,3-氨基变位酶（AAM）活性的多肽，编码AAM多肽的多核苷酸，以及用于表达AAM多肽的表达载体和宿主细胞。

公开(公告)号：US07790432B2

公开(公告)日：2010-09-07

申请号：US11919271

申请日：2005-10-25

Applicant: Ranjini Chatterjee ,  Kenneth W. Mitchell ,  Susan Y. Louie ,  Richard J. Fox ,  Michelle Chen

Inventor： Ranjini Chatterjee ,  Kenneth W. Mitchell ,  Susan Y. Louie ,  Richard J. Fox ,  Michelle Chen

IPC: C12N9/88 ,  C12N1/20 ,  C12N1/00 ,  C12N15/00 ,  C07H21/02

CPC classification number: C12N9/90

Abstract: The present invention is directed to polypeptides that have enhanced alanine 2,3-aminomutase (AAM) activity and/or thermostability relative to the wild-type enzymes that have incidental AAM activity as a result of cross reactivity with alanine. In addition, the present invention is directed to a polynucleotides that encodes for the AAM polypeptides of the present invention, to nucleic acid sequences comprising the polynucleotides, to expression vectors comprising the polynucleotides operatively linked to a promoter, to host cells transformed to express the AAM polypeptides, and to a method for producing the AAM polypeptides of the present invention.

Abstract translation: 本发明涉及相对于具有与丙氨酸交叉反应性的结果具有附带的AAM活性的野生型酶具有增强的丙氨酸2,3-氨基变位酶（AAM）活性和/或热稳定性的多肽。 此外，本发明涉及编码本发明的AAM多肽的多核苷酸，包含多核苷酸的核酸序列与包含与启动子可操作连接的多核苷酸的表达载体，转染以表达AAM的宿主细胞 多肽，以及用于制备本发明的AAM多肽的方法。

公开(公告)号：US20120202272A1

公开(公告)日：2012-08-09

申请号：US13220658

申请日：2011-08-29

Applicant: Ranjini Chatterjee ,  Kenneth Zahn ,  Kenneth Mitchell ,  Yaoquan Liu

Inventor： Ranjini Chatterjee ,  Kenneth Zahn ,  Kenneth Mitchell ,  Yaoquan Liu

IPC: C12N1/21

CPC classification number: C12N9/1088 ,  C12N9/88 ,  C12P7/22 ,  C12P7/26 ,  C12P7/42 ,  C12Y205/01018

Abstract: The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes.

Abstract translation: 本文提供的教导通常涉及使用酶促生物转化过程将木质素衍生的化合物转化为有价值的芳族化学品的方法。 该教导提供（i）对木质素部分中存在的有毒化合物具有耐受性的宿主细胞的选择; （ii）在木质素部分生物转化成芳族化学产品时可用作酶的多肽; （iii）可用于转化宿主细胞以表达多肽选择的多核苷酸，作为木质素级分的生物转化中的酶; 和（iv）表达酶的转化体。

公开(公告)号：US20120202258A1

公开(公告)日：2012-08-09

申请号：US13220646

申请日：2011-08-29

Applicant: Ranjini Chatterjee ,  Kenneth Zahn ,  Kenneth Mitchell ,  Yaoquan Liu

Inventor： Ranjini Chatterjee ,  Kenneth Zahn ,  Kenneth Mitchell ,  Yaoquan Liu

IPC: C12P13/00 ,  C12N15/63 ,  C12P7/42 ,  C12P7/22 ,  C12P7/24 ,  C12N15/54 ,  C12N1/21

CPC classification number: C12N9/1088 ,  C12N9/88 ,  C12P7/22 ,  C12P7/26 ,  C12P7/42 ,  C12Y205/01018

Abstract: The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes.

Abstract translation: 本文提供的教导通常涉及使用酶促生物转化过程将木质素衍生的化合物转化为有价值的芳族化学品的方法。 该教导提供（i）对木质素部分中存在的有毒化合物具有耐受性的宿主细胞的选择; （ii）在木质素部分生物转化成芳族化学产品时可用作酶的多肽; （iii）可用于转化宿主细胞以表达多肽选择的多核苷酸，作为木质素级分的生物转化中的酶; 和（iv）表达酶的转化体。

公开(公告)号：US08030051B2

公开(公告)日：2011-10-04

申请号：US11581946

申请日：2006-10-16

Applicant: Svetlana Balatskaya ,  Birthe Borup ,  Ranjini Chatterjee ,  Ish Dhawan ,  Richard J. Fox ,  Kenneth W. Mitchell ,  Emily C. Mundorff ,  Les Partridge ,  Matthew Tobin

Inventor： Svetlana Balatskaya ,  Birthe Borup ,  Ranjini Chatterjee ,  Ish Dhawan ,  Richard J. Fox ,  Kenneth W. Mitchell ,  Emily C. Mundorff ,  Les Partridge ,  Matthew Tobin

IPC: C12N9/90 ,  C12N1/20 ,  C12N15/70 ,  C12P13/06

CPC classification number: C12N9/90 ,  C12P13/06

Abstract: The present disclosure relates generally to polypeptides having improved alanine 2,3-aminomutase (AAM) activity, the polynucleotides encoding the AAM polypeptides, and expression vectors and host cells for expressing the AAM polypeptides.

Abstract translation: 本公开一般涉及具有改进的丙氨酸2,3-氨基变位酶（AAM）活性的多肽，编码AAM多肽的多核苷酸，以及用于表达AAM多肽的表达载体和宿主细胞。

公开(公告)号：US07745186B2

公开(公告)日：2010-06-29

申请号：US12049839

申请日：2008-03-17

Applicant: Ranjini Chatterjee ,  Michelle M. Chen ,  Ranjan Patnaik ,  Stephen L. Schmidt

Inventor： Ranjini Chatterjee ,  Michelle M. Chen ,  Ranjan Patnaik ,  Stephen L. Schmidt

IPC: C12N9/02 ,  C12N9/10 ,  C12N9/00 ,  C12N15/00 ,  C12P21/06 ,  C07H21/04

CPC classification number: C12N9/88

Abstract: The present invention relates to novel ribulose-1,5-bisphosphate carboxylase/oxygenase polypeptides and the polynucleotides that encode them. The invention also provides related host cells and methods.

Abstract translation: 本发明涉及新的核酮糖-1,5-二磷酸羧化酶/加氧酶多肽和编码它们的多核苷酸。 本发明还提供相关的宿主细胞和方法。

公开(公告)号：US07351562B2

公开(公告)日：2008-04-01

申请号：US10425332

申请日：2003-04-28

Applicant: Ranjini Chatterjee ,  Michelle M. Chen ,  Ranjan Patnaik ,  Stephen L. Schmidt

Inventor： Ranjini Chatterjee ,  Michelle M. Chen ,  Ranjan Patnaik ,  Stephen L. Schmidt

IPC: C12N9/12 ,  C12N15/00 ,  C12P21/06 ,  C07H21/04

CPC classification number: C12N9/88

Abstract: The present invention relates to novel ribulose-1,5-bisphosphate carboxylase/oxygenase polypeptides and the polynucleotides that encode them. The invention also provides related host cells and methods.

Abstract translation: 本发明涉及新的核酮糖-1,5-二磷酸羧化酶/加氧酶多肽和编码它们的多核苷酸。 本发明还提供相关的宿主细胞和方法。

公开(公告)号：US6159738A

公开(公告)日：2000-12-12

申请号：US67931

申请日：1998-04-28

Applicant: Mark I. Donnelly ,  Cynthia Sanville-Millard ,  Ranjini Chatterjee

Inventor： Mark I. Donnelly ,  Cynthia Sanville-Millard ,  Ranjini Chatterjee

IPC: C12N15/70 ,  C12N15/90 ,  C12P7/46 ,  C12N15/00 ,  C12N15/74 ,  C12P7/00

CPC classification number: C12P7/46 ,  C12N15/70 ,  C12N15/902

Abstract: A fermentation process for producing succinic acid is provided comprising selecting a bacterial strain that does not produce succinic acid in high yield, disrupting the normal regulation of sugar metabolism of said bacterial strain, and combining the mutant bacterial strain and selected sugar in anaerobic conditions to facilitate production of succinic acid. Also provided is a method for changing low yield succinic acid producing bacteria to high yield succinic acid producing bacteria comprising selecting a bacterial strain having a phosphotransferase system and altering the phosphotransferase system so as to allow the bacterial strain to simultaneously metabolize different sugars.

Abstract translation: 提供了用于制备琥珀酸的发酵方法，其包括选择不以高产率生产琥珀酸的细菌菌株，破坏所述细菌菌株的糖代谢的正常调节，以及在厌氧条件下组合突变细菌菌株和选择的糖以促进 生产琥珀酸。 还提供了一种将低产琥珀酸生产细菌转化为高产琥珀酸生产细菌的方法，包括选择具有磷酸转移酶系统的细菌菌株和改变磷酸转移酶体系，以使细菌菌株同时代谢不同的糖。