公开(公告)号：US20070292879A1

公开(公告)日：2007-12-20

申请号：US11781986

申请日：2007-07-24

Applicant: ANUP SOOD ,  SHIV KUMAR ,  CARL FULLER ,  JOHN NELSON

Inventor： ANUP SOOD ,  SHIV KUMAR ,  CARL FULLER ,  JOHN NELSON

IPC: C12Q1/68

CPC classification number: C07H19/20 ,  C07H19/10 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6851 ,  C12Q1/6869 ,  C12Q2525/186

Abstract: The present invention describes terminal phosphate blocked nucleoside polyphosphates that are stable at high temperature and their use in nucleic acid amplification and analysis. Current invention further describes charge modified terminal phosphate blocked nucleoside polyphosphates for improved incorporation and direct loading of nucleic acid sequencing reactions onto separating media.

Abstract translation: 本发明描述了在高温下稳定的磷酸封端的磷酸核苷多磷酸，并且其用于核酸扩增和分析。 本发明进一步描述了电荷修饰的末端磷酸酯封闭的核苷多磷酸盐，用于改进核酸测序反应在分离介质上的掺入和直接加载。

公开(公告)号：US07217726B2

公开(公告)日：2007-05-15

申请号：US10469648

申请日：2003-08-21

Applicant: Shiv Kumar Agarwal ,  Mrinal Kanti Guha ,  Surendrakumar Satyanarayan Pandey ,  Matte Marianna Samuel

Inventor： Shiv Kumar Agarwal ,  Mrinal Kanti Guha ,  Surendrakumar Satyanarayan Pandey ,  Matte Marianna Samuel

IPC: C07D413/10 ,  A61K31/422

CPC classification number: C07D263/20 ,  C07D413/12

Abstract: The present invention provides novel compounds of the general formula (I), their derivatives, their analogs, their tautomeric forms, their stereoisomers, their polymorphs, their hydrates, their solvates, their pharmaceutically acceptable salts and pharmaceutically acceptable compositions containing them. The present invention more particularly provides novel oxazolidinone derivatives of the general formula (I)

Abstract translation: 本发明提供了通式（I）的新化合物，它们的衍生物，它们的类似物，它们的互变异构形式，它们的立体异构体，它们的多晶型物，它们的水合物，它们的溶剂合物，它们的药学上可接受的盐和含有它们的药学上可接受的组合物。 本发明更具体地提供了通式（I）的新的恶唑烷酮衍生物，

公开(公告)号：US07033762B2

公开(公告)日：2006-04-25

申请号：US10113025

申请日：2002-04-01

Applicant: John Nelson ,  Carl Fuller ,  Anup Sood ,  Shiv Kumar

Inventor： John Nelson ,  Carl Fuller ,  Anup Sood ,  Shiv Kumar

IPC: C12Q1/68

CPC classification number: C12Q1/6823 ,  C12Q2545/114 ,  C12Q2521/525 ,  C12Q2521/101

Abstract: A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, a non-hydrolyzable primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and an enzyme having 3′→5′ exonuclease activity which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species characteristic of the sample; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on the detection.

公开(公告)号：US06855503B2

公开(公告)日：2005-02-15

申请号：US10326039

申请日：2002-12-20

Applicant: Satyam Nampalli ,  Weihong Zhang ,  Sudhakar Rao ,  Shiv Kumar

Inventor： Satyam Nampalli ,  Weihong Zhang ,  Sudhakar Rao ,  Shiv Kumar

IPC: C09B11/08 ,  C09B11/24 ,  C09B23/08 ,  C12Q1/68 ,  C07D1/02

CPC classification number: C09B23/083 ,  C09B11/08 ,  C09B11/24 ,  C12Q1/6818 ,  C12Q1/6869

Abstract: Exploitation of suitably functionalized heterocyclic molecules, in the design and synthesis of Fluorescence Resonance Energy Transfer (FRET) cassettes and their corresponding dideoxynucleotide terminators culminated into efficient reagents for DNA sequencing. Additionally, these FRET cassettes/terminators, of the present invention, derived from different classes of heterocyclic systems have high potential to be used for general labelling of biological molecules to generate highly sensitized signals. Their preparation, energy transfer efficiency, and use as labels, specifically, in DNA sequencing reactions is disclosed.

Abstract translation: 在荧光共振能量转移（FRET）盒及其相应的双脱氧核苷酸终止子的设计和合成中，适当官能化的杂环分子的开发最终成为DNA测序的有效试剂。 此外，衍生自不同种类的杂环体系的本发明的这些FRET盒/终止子具有很高的潜力用于生物分子的一般标记以产生高致敏信号。 公开了它们的制备，能量转移效率和用作标记，特别是在DNA测序反应中。

公开(公告)号：US06600028B1

公开(公告)日：2003-07-29

申请号：US09402048

申请日：2000-02-01

Applicant: Daniel Brown ,  David Loakes ,  David Williams ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall ,  Alan Hamilton ,  Clifford Smith ,  Adrian Christopher Simmonds ,  William Jonathan Cummins ,  Patrick Finn

Inventor： Daniel Brown ,  David Loakes ,  David Williams ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall ,  Alan Hamilton ,  Clifford Smith ,  Adrian Christopher Simmonds ,  William Jonathan Cummins ,  Patrick Finn

IPC: C07H2100

CPC classification number: C07D498/16 ,  C07H19/04 ,  C07H21/00

Abstract: Nucleoside analogues have structure (2) wherein Q is H or a sugar moiety or sugar analogue or a modified sugar or a nucleic backbone or backbone analogue, W is an alkylene or alkenylene chain of 0-5 carbon atoms, any of which may carry a substituent R8, X is O or N or NR12 or CR10, X′ is O or S or N, provided that when X′ is O or S, then X is C, Y is CH or N, R6 is NH2 or SMe or SO2Me or NHNH2, each of R7 and R8 is independently H or F or alkyl or alkenyl or aryl or acyl or a reporter moiety, R12 is independently H or alkyl or alkenyl or aryl or acyl or a reporter moiety, and R10 is H or ═O or F or alkyl or aryl or a reporter moiety.

Abstract translation: 核苷类似物具有结构（2），其中Q是H或糖部分或糖类似物或修饰的糖或核心或骨架类似物，W是0-5个碳原子的亚烷基或亚烯基链，其中任何一个可携带 取代基R8，X为O或N或NR12或CR10，X'为O或S或N，条件是当X'为O或S时，则X为C，Y为CH或N，R6为NH2或SMe或SO2Me 或NHNH 2，R 7和R 8各自独立地为H或F，或烷基或链烯基或芳基或酰基或报道部分，R 12独立地为H或烷基或链烯基或芳基或酰基或报道部分，R 10为H或= O 或F或烷基或芳基或报道部分。

公开(公告)号：US06444682B1

公开(公告)日：2002-09-03

申请号：US09463501

申请日：2000-04-18

Applicant: Adrian Christopher Simmonds ,  Alan Hamilton ,  Clifford Smith ,  David Loakes ,  Daniel Brown ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall

Inventor： Adrian Christopher Simmonds ,  Alan Hamilton ,  Clifford Smith ,  David Loakes ,  Daniel Brown ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall

IPC: A61K3170

CPC classification number: G01N33/5308 ,  C07H21/00

Abstract: Nucleotide or base analogues having structure (3) or (4) wherein X═O or NH or S and each R6 is independently H or alkyl or alkenyl or alkoxy or aryl or a reporter moiety.

Abstract translation: 具有结构（3）或（4）的核苷酸或碱基类似物，其中X = O或NH或S，每个R6独立地为H或烷基或链烯基，烷氧基或芳基或报道部分。

公开(公告)号：US11795191B2

公开(公告)日：2023-10-24

申请号：US16371646

申请日：2019-04-01

Applicant: Jingyue Ju ,  Shiv Kumar ,  Chuanjuan Tao ,  Minchen Chien ,  James J. Russo ,  John J. Kasianowicz ,  Joseph W. F. Robertson

Inventor： Jingyue Ju ,  Shiv Kumar ,  Chuanjuan Tao ,  Minchen Chien ,  James J. Russo ,  John J. Kasianowicz ,  Joseph W. F. Robertson

IPC: C12Q1/68 ,  C07H19/00 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/6869 ,  C07H19/06 ,  C07H19/16 ,  G01N33/487 ,  C12Q1/6883 ,  B82Y5/00

CPC classification number: C07H19/00 ,  C07H19/06 ,  C07H19/16 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/6869 ,  C12Q1/6883 ,  G01N33/48721 ,  B82Y5/00 ,  C12Q2600/158 ,  G01N2333/9125 ,  C12Q1/6869 ,  C12Q2521/525 ,  C12Q2521/543 ,  C12Q2537/157 ,  C12Q2565/631 ,  C12Q1/6869 ,  C12Q2521/525 ,  C12Q2521/543 ,  C12Q2535/107 ,  C12Q2565/631 ,  C12Q1/6869 ,  C12Q2565/631

Abstract: This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.

公开(公告)号：US20230172961A1

公开(公告)日：2023-06-08

申请号：US17796629

申请日：2021-01-29

Applicant: Jingyue JU ,  Steffen JOCKUSCH ,  Chuanjuan TAO ,  Minchen CHIEN ,  James J. RUSSO ,  Shiv KUMAR ,  Xiaoxu LI ,  Xuanting WANG

Inventor： Jingyue Ju ,  Steffen Jockusch ,  Chuanjuan Tao ,  Minchen Chien ,  James J. Russo ,  Shiv Kumar ,  Xiaoxu Li ,  Xuanting Wang

IPC: A61K31/7072 ,  A61K31/7076 ,  A61K31/708 ,  A61K31/7068 ,  A61K45/06

CPC classification number: A61K31/7072 ,  A61K31/708 ,  A61K31/7068 ,  A61K31/7076 ,  A61K45/06

Abstract: This invention relates to the use of nucleoside, nucleotide and other compounds which are inhibitors or terminators of viral RNA dependent RNA polymerases or inhibitors of exonucleases as antiviral agents. These antiviral agents can be used alone or in combination with other polymerase or exonuclease inhibitors, helicase inhibitors, HCV NS5A inhibitors, HIV integrase inhibitors and HCV NS3-4A and other protease inhibitors to treat viral infections such as SARS-CoV-2, the causative agent of the COVID-19 infection.

公开(公告)号：US20220213542A1

公开(公告)日：2022-07-07

申请号：US17600593

申请日：2020-04-02

Applicant: Jingyue Ju ,  Shiv Kumar ,  James Russo ,  Xiaoxu Li ,  Steffen Jockusch ,  Minchen Chien ,  Chuanjuan Tao

Inventor： Jingyue Ju ,  Shiv Kumar ,  James Russo ,  Xiaoxu Li ,  Steffen Jockusch ,  Minchen Chien ,  Chuanjuan Tao

IPC: C12Q1/6869 ,  C12Q1/6848

Abstract: The invention provides nucleotide analogues and methods of use thereof for sequencing by synthesis using energy transfer dye pairs.

公开(公告)号：US20210285041A1

公开(公告)日：2021-09-16

申请号：US16498132

申请日：2018-03-28

Applicant: Jingyue Ju ,  Xiaoxu Li ,  Xin Chen ,  Shiv Kumar ,  James Russo ,  Minchen Chien

Inventor： Jingyue Ju ,  Xiaoxu Li ,  Xin Chen ,  Shiv Kumar ,  James Russo ,  Minchen Chien

IPC: C12Q1/6869 ,  C07H19/10 ,  C07H19/20

Abstract: Disclosed herein, inter alia, are nucleotide analogues, and methods of use thereof, having cleavable orthogonal linkers.