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    Method of Controlling Plant Growth and Architecture by Controlling Expression of Gibberellin 2-Oxidase
    13.
    发明申请
    Method of Controlling Plant Growth and Architecture by Controlling Expression of Gibberellin 2-Oxidase 有权
    通过控制赤霉素2氧化酶的表达控制植物生长和结构的方法

    公开(公告)号：US20100095406A1

    公开(公告)日：2010-04-15

    申请号：US12604808

    申请日：2009-10-23

    Applicant: Su-May YU ,  Shuen-Fang LO ,  Liang-Jwu CHEN

    Inventor： Su-May YU ,  Shuen-Fang LO ,  Liang-Jwu CHEN

    IPC: C12N15/82

    CPC classification number: C12N15/8297 ,  C12N9/0071 ,  C12N15/8273

    Abstract: Novel gibberellin 2-oxidase (GA2ox) genes were identified. Differential expression of GA2ox genes correlated with flower development, seed germination, tiller growth and other developmental processes. In addition, the early and increased growth of tiller and adventitious root and altered root architecture caused by overexpression of GA2oxs further suggest the pleiotropic role of GA2oxs in controlling growth and architecture in plants such as rice. GA2ox5, GA2ox6 and GA2ox9 were three genes encoding class C20 GA2oxs in rice. Mutants or transgenic rice overexpressing class C20 GA2oxs exhibited a broad range of mutant phenotypes, including semi-dwarfism, increased root system and higher tiller numbers that may favor grain yield. Mutations in the conserved domain III were found to affect the physiological activity of class C20 GA2oxs. Methods are described for controlling plant growth and architecture by controlling gene expression of gibberellin 2-oxidase in the plant.

    Abstract translation: 鉴定了新型赤霉素2-氧化酶（GA2ox）基因。 GA2ox基因的差异表达与花的发育，种子发芽，分蘖生长等发育过程相关。 此外，由于GA2ox过表达引起的分蘖和不定根的早期增长和根系的改变进一步表明GA2oxs在控制水稻等植物生长和结构方面具有多方位的作用。 GA2ox5，GA2ox6和GA2ox9是水稻中编码C20 GA2oxs的三个基因。 突变体或转基因水稻超过C20类型的GA2oxs表现出广泛的突变表型，包括半侏儒症，增加的根系和较高的分蘖数，可能有利于谷物产量。 发现保守结构域III中的突变会影响C20类GA2氧化酶的生理活性。 描述了通过控制植物中赤霉素2-氧化酶的基因表达来控制植物生长和结构的方法。

    Rice Glutelin Gene Promoters
    14.
    发明申请
    Rice Glutelin Gene Promoters 有权
    水稻谷蛋白基因启动子

    公开(公告)号：US20080250528A1

    公开(公告)日：2008-10-09

    申请号：US12053792

    申请日：2008-03-24

    Applicant: Su-may Yu ,  Chwan-yang Hong

    Inventor： Su-may Yu ,  Chwan-yang Hong

    IPC: C12N15/11 ,  C07H21/00 ,  C12N15/82 ,  C12N5/10 ,  A01H5/00

    CPC classification number: C12N15/8234 ,  C12N15/8257

    Abstract: A nucleic acid containing a glutelin gene promoter. Disclosed are transformed plant cells and transgenic plants containing a nucleic acid that includes the promoter operably linked to a sequence encoding heterologous protein. Also disclosed are methods of making the transformed plant cells and transgenic plants and methods for expressing a polypeptide.

    Abstract translation: 含有谷蛋白基因启动子的核酸。 公开了转化的植物细胞和含有核酸的转基因植物，所述核酸包括可操作地连接到编码异源蛋白质的序列的启动子。 还公开了制备转化的植物细胞和转基因植物的方法以及用于表达多肽的方法。

    Aarobacterium-mediated method for transforming rice
    15.
    发明授权
    Aarobacterium-mediated method for transforming rice 失效
    农杆菌介导的水稻转化方法

    公开(公告)号：US06215051B1

    公开(公告)日：2001-04-10

    申请号：US09072435

    申请日：1998-05-04

    Applicant: Su-May Yu ,  Li-Fei Liu ,  Ming-Tsair Chan

    Inventor： Su-May Yu ,  Li-Fei Liu ,  Ming-Tsair Chan

    IPC: C12N1529

    CPC classification number: C12N15/8222 ,  C12N15/8234 ,  C12N15/8238

    Abstract: The present invention is directed to a method for the production of a transgenic plant of rice crop comprising the steps of infecting an immature embryo of rice crop with the genus Agrobacterium for transformation; co-culturing the infected embryo with a dicot suspension culture during the step of transformation; allowing the transformed embryo to grow into a callus in a selective medium comprising a sufficient amount of a plant growth hormone for the growth of rice crop; and allowing the cultured callus to regenerate root and shoot in a regeneration medium comprising a pre-determined amount of nutrients for the growth of rice crop. The invention is further directed to a transformed rice plant made by methods of this invention.

    Abstract translation: 本发明涉及一种用于生产水稻作物转基因植物的方法，包括用农杆菌感染水稻作物的未成熟胚进行转化的步骤; 在转化步骤期间用双子叶悬浮培养物共同培养感染的胚胎; 使得转化的胚胎在包含足够量的用于水稻作物生长的植物生长激素的选择培养基中生长成愈伤组织; 并且允许培养的愈伤组织在包含用于水稻作物生长的预定量的营养物质的再生培养基中再生根和芽。 本发明进一步涉及通过本发明的方法制备的转化水稻植物。

    Rice glutelin gene promoters
    17.
    发明授权
    Rice glutelin gene promoters 有权
    水稻谷蛋白基因启动子

    公开(公告)号：US07928293B2

    公开(公告)日：2011-04-19

    申请号：US12053792

    申请日：2008-03-24

    Applicant: Su-may Yu ,  Chwan-yang Hong

    Inventor： Su-may Yu ,  Chwan-yang Hong

    IPC: A01H1/00 ,  C07H21/04 ,  C12N15/00

    CPC classification number: C12N15/8234 ,  C12N15/8257

    Abstract: A nucleic acid containing a glutelin gene promoter. Disclosed are transformed plant cells and transgenic plants containing a nucleic acid that includes the promoter operably linked to a sequence encoding heterologous protein. Also disclosed are methods of making the transformed plant cells and transgenic plants and methods for expressing a polypeptide.

    Abstract translation: 含有谷蛋白基因启动子的核酸。 公开了转化的植物细胞和含有核酸的转基因植物，所述核酸包括可操作地连接到编码异源蛋白质的序列的启动子。 还公开了制备转化的植物细胞和转基因植物的方法以及用于表达多肽的方法。

    Transgenic seeds expressing amylopullulanase and uses therefor
    19.
    发明授权
    Transgenic seeds expressing amylopullulanase and uses therefor 有权
    转基因种子表达amylopullululanase和用途

    公开(公告)号：US06737563B2

    公开(公告)日：2004-05-18

    申请号：US10050763

    申请日：2002-01-16

    Applicant: Su-May Yu ,  Jei-Fu Shaw

    Inventor： Su-May Yu ,  Jei-Fu Shaw

    IPC: C12N1582

    CPC classification number: C12N15/8251 ,  C12N15/8245 ,  C12P19/16

    Abstract: The invention provides DNA constructs and genetically engineered seeds for the expression of amylopullulanase in plant seeds such as rice seeds. Related methods are also provided for the production of sugars, modified starches, and high protein products, and use of the glutelin promoter in the methods.

    Abstract translation: 本发明提供DNA构建体和用于在植物种子例如水稻种子中表达淀粉样蛋白酶的基因工程种子。 还提供了相关方法用于生产糖，改性淀粉和高蛋白产物，以及在该方法中使用谷蛋白启动子。

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