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11.发明申请公开(公告)号：US20080125326A1
公开(公告)日：2008-05-29
申请号：US11779915
申请日：2007-07-19
Applicant: Narendra S. Yadav , Zhixiong Xue , Quinn Qun Zhu
Inventor： Narendra S. Yadav , Zhixiong Xue , Quinn Qun Zhu
IPC: C12P17/08 , C07H21/04 , C12N1/00 , C12N1/12 , C12Q1/68 , C40B30/04 , C12N1/20 , C12N5/06 , C12N9/00
CPC classification number: C12P7/6427 , C12N9/0083 , C12P7/6472
Abstract: The present invention relates to Δ17 desaturases, which have the ability to convert ω-6 fatty acids into their ω-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ17 desaturases in oleaginous yeast are disclosed.
Abstract translation: 本发明涉及具有将ω-6脂肪酸转化成其ω-3对应物的能力的Delta17去饱和酶（即将花生四烯酸[20：4，ARA]转化为二十碳五烯酸[20：5，EPA]） 。 公开了分离的核酸片段和包含编码Δ17去饱和酶的片段的重组构建物以及使用这些Delta17去饱和酶在含油酵母中制备长链多不饱和脂肪酸（PUFA）的方法。
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12.发明申请公开(公告)号：US20080020415A1
公开(公告)日：2008-01-24
申请号：US11830023
申请日：2007-07-30
Applicant: Narendra Yadav , Zhixiong Xue , Hongxiang Zhang
Inventor： Narendra Yadav , Zhixiong Xue , Hongxiang Zhang
CPC classification number: C12N9/1029 , C12P7/6463 , C12P7/6472
Abstract: Acyltransferases are provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous yeast (e.g., Yarrowia lipolytica). Specifically, genes encoding diacylglycerol acyltransferase (DGAT1) have been isolated from Y. lipolytica and Mortierella alpina. These genes encode enzymes that participate in the terminal step in oil biosynthesis in yeast. Each is expected to play a key role in altering the quantity of polyunsaturated fatty acids produced in oils of oleaginous yeasts.
Abstract translation: 提供了酰基转移酶，适用于制造含油酵母（例如解脂耶氏酵母）富含ω脂肪酸的微生物油。 具体来说，从解脂耶氏酵母和高山被孢霉中分离出编码二酰基甘油酰基转移酶（DGAT1）的基因。 这些基因编码酵母中参与油生物合成的最终步骤的酶。 预计每一种在改变油质酵母油中产生的多不饱和脂肪酸的量方面起关键作用。
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13.发明授权Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast 有权甘油-3-磷酸O-酰基转移酶启动子用于油质酵母中的基因表达公开(公告)号：US07264949B2
公开(公告)日：2007-09-04
申请号：US11225354
申请日：2005-09-13
Applicant: Zhixiong Xue , Quinn Qun Zhu
Inventor： Zhixiong Xue , Quinn Qun Zhu
CPC classification number: C12N15/815 , C12N9/1029 , C12P7/6427 , C12P21/02 , C12Y203/01015
Abstract: The promoter region associated with the Yarrowia lipolytica glycerol-3-phosphate O-acyltransferase (gpat) gene has been found to be particularly effective for the expression of heterologous genes in oleaginous yeast. The promoter regions of the instant invention have been shown to be suitable to drive high-level expression of genes involved in the production of ω-3 and ω-6 fatty acids.
Abstract translation: 已经发现与解脂耶氏酵母甘油-3-磷酸O-酰基转移酶（gpat）基因相关的启动子区域在含油酵母中表达异源基因特别有效。 已经显示本发明的启动子区域适合于驱动涉及ω-3和ω-6脂肪酸生产的基因的高水平表达。
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公开(公告)号：US20060110806A1
公开(公告)日：2006-05-25
申请号：US11264737
申请日：2005-11-01
Applicant: Howard Damude , Peter Gillies , Daniel Macool , Stephen Picataggio , James Ragghianti , John Seip , Zhixiong Xue , Narendra Yadav , Hongxiang Zhang , Quinn Zhu
Inventor： Howard Damude , Peter Gillies , Daniel Macool , Stephen Picataggio , James Ragghianti , John Seip , Zhixiong Xue , Narendra Yadav , Hongxiang Zhang , Quinn Zhu
CPC classification number: C12P7/6427 , A23D9/00 , A23K20/158 , A23L33/12 , A23V2002/00 , C12N1/16 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12N15/746 , C12N15/815 , C12P7/6472 , A23V2250/187 , A23V2200/30
Abstract: An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.
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15.发明申请Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 失效高山被孢霉甘油-3-磷酸O-酰基转移酶用于改变多不饱和脂肪酸和含油生物中的油含量公开(公告)号：US20060094091A1
公开(公告)日：2006-05-04
申请号：US11254173
申请日：2005-10-19
Applicant: Daniel Macool , Zhixiong Xue
Inventor： Daniel Macool , Zhixiong Xue
CPC classification number: C12P7/6472 , C12N9/1029 , C12P7/6463
Abstract: Glycerol-3-phosphate o-acyltransferase (GPAT) participates in the first step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids (PUFAs) produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment isolated from Mortierella alpina encoding a GPAT that is suitable for use in the manufacture of oils enriched in omega fatty acids in oleaginous organisms. Most desirably, the substrate specificity of the instant GPAT will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.
Abstract translation: 甘油-3-磷酸O-酰基转移酶（GPAT）参与石油生物合成的第一步，预计在改变含油生物油中产生的长链多不饱和脂肪酸（PUFA）的量方面发挥关键作用。 本申请提供了从编码GPAT的高山被孢霉分离的核酸片段，该GPAT适用于制造含油生物体中富含ω-脂肪酸的油。 最理想的是，本发明的GPAT的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母（Yarrowia lipolytica）中积累具有等于或大于C 20的链长度的长链PUFA。
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Patent Agency Ranking
16.发明申请Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms 有权高山被孢霉溶血磷脂酸酰基转移酶同源物，用于改变多不饱和脂肪酸和含油生物中的油含量公开(公告)号：US20060094090A1
公开(公告)日：2006-05-04
申请号：US11251466
申请日：2005-10-14
Applicant: Howard Damude , Zhixiong Xue
Inventor： Howard Damude , Zhixiong Xue
CPC classification number: C12P7/6472 , C12N9/1029 , C12P7/6463
Abstract: Lysophosphatidic acid acyltransferase (LPAAT) participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment (identified as “LPAAT2”) isolated from Mortierella alpina encoding a LPAAT homolog that is suitable for use in the manufacture of oils enriched in omega fatty acids in oleaginous organisms. Most desirably, the substrate specificity of the instant LPAAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.
Abstract translation: 溶血磷脂酰基转移酶（LPAAT）参与石油生物合成的第二步，预计在改变含油生物油中产生的长链多不饱和脂肪酸的量方面起关键作用。 本申请提供了从编码LPAAT同源物的高山被孢霉分离的核酸片段（鉴定为“LPAAT2”），其适用于制造含油生物体中富含ω-脂肪酸的油。 最理想的是，本发明的LPAAT2的底物特异性将特别适用于在含油酵母例如解脂耶氏酵母中积累具有等于或大于C 20的链长的长链PUFA。
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17.发明授权Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids 有权突变型HPGG基序和HDASH基序delta-5去饱和酶及其在制备多不饱和脂肪酸中的应用公开(公告)号：US08658413B2
公开(公告)日：2014-02-25
申请号：US13218673
申请日：2011-08-26
Applicant: Melissa D. Bosak , Michael W. Bostick , Hongxian He , Seung-Pyo Hong , Dana M. Walters Pollak , Pamela L. Sharpe , Yehong Jamie Wang , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
Inventor： Melissa D. Bosak , Michael W. Bostick , Hongxian He , Seung-Pyo Hong , Dana M. Walters Pollak , Pamela L. Sharpe , Yehong Jamie Wang , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC classification number: C12N9/001 , C12P7/6472 , C12Y103/01035
Abstract: Mutant delta-5 desaturases, having the ability to convert dihomo-gamma-linolenic acid [DGLA; 20:3 omega-6] to arachidonic acid [ARA; 20:4 omega-6] and/or eicosatetraenoic acid [ETA; 20:4 omega-3] to eicosapentaenoic acid [EPA; 20:5 omega-3] and possessing at least one mutation within the HPGG (SEQ ID NO:7) motif of the cytochome b5-like domain and at least one mutation within the HDASH (SEQ ID NO:8) motif are disclosed. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”], are also disclosed.
Abstract translation: 具有将dihomo-γ-亚麻酸转化的能力的变体delta-5去饱和酶[DGLA; 20：3ω-6]花生四烯酸[ARA; 20：4ω-6]和/或二十碳四烯酸[ETA; 20：4ω-3]至二十碳五烯酸[EPA; 20：5ω-3]，并且在细胞色素b5样结构域的HPGG（SEQ ID NO：7）基序和HDASH（SEQ ID NO：8）基序内的至少一个突变中具有至少一个突变。 还公开了分离的核酸片段和包含编码Δ-5去饱和酶的片段的重组构建物，以及制备长链多不饱和脂肪酸[“PUFA”]的方法。
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18.发明申请DELTA 17 DESATURASE AND ITS USE IN MAKING POLYUNSATURATED FATTY ACIDS 审中-公开DELTA 17去饱和及其在制备聚氨酯脂肪酸中的应用公开(公告)号：US20130011846A1
公开(公告)日：2013-01-10
申请号：US13535775
申请日：2012-06-28
Applicant: Narendra S. Yadav , Zhixiong Xue , Quinn Qun Zhu
Inventor： Narendra S. Yadav , Zhixiong Xue , Quinn Qun Zhu
CPC classification number: C12P7/6427 , C12N9/0083 , C12P7/6472
Abstract: The present invention relates to Δ17 desaturases, which have the ability to convert ω-6 fatty acids into their ω-3 counterparts (i.e., conversion of arachidonic acid [20:4, ARA] to eicosapentaenoic acid [20:5, EPA]). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ17 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ17 desaturases in oleaginous yeast are disclosed.
Abstract translation: 本发明涉及具有将ω-6脂肪酸转化成其ω-3对应物的能力（即将花生四烯酸[20:4，ARA]转化为二十碳五烯酸[20:5，EPA ]）。 公开了分离的核酸片段和重组构建体，其包含编码＆amp; 17去饱和酶的这种片段以及使用这些在油性酵母中的这些＆Dgr.17去饱和酶制备长链多不饱和脂肪酸（PUFA）的方法。
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公开(公告)号：US08313911B2
公开(公告)日：2012-11-20
申请号：US12641929
申请日：2009-12-18
Applicant: Ethel Noland Jackson , David Richard Short , Dongming Xie , Zhixiong Xue , Quinn Qun Zhu
Inventor： Ethel Noland Jackson , David Richard Short , Dongming Xie , Zhixiong Xue , Quinn Qun Zhu
CPC classification number: C12P7/6427
Abstract: Methods for the production of omega-3 and/or omega-6 fatty acids in oleaginous yeasts grown on a fermentable carbon source selected from the group consisting of invert sucrose, glucose, fructose and combinations of these, provided that glucose is used in combination with invert sucrose and/or fructose. Specifically, methods are provided for production of linoleic acid, eicosadienoic acid, gamma-linolenic acid, dihomo-gamma-linolenic acid, arachidonic acid, n-6 docosapentaenoic acid, α-linolenic acid, stearidonic acid, eicosatrienoic acid, eicosatetraenoic acid, eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid.
Abstract translation: 在选自蔗糖，葡萄糖，果糖及其组合的可发酵碳源上生长的油质酵母中生产ω-3和/或ω-6脂肪酸的方法，其中葡萄糖与 反转蔗糖和/或果糖。 具体地说，提供了用于生产亚油酸，二十碳二烯酸，γ-亚麻酸，二高-γ-亚麻酸，花生四烯酸，n-6二十二碳五烯酸，α-亚麻酸，十八碳四烯酸，二十碳三烯酸，二十碳四烯酸，二十碳五烯酸 酸，二十二碳五烯酸和二十二碳六烯酸。
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20.发明申请YARROWIA DIACYLGLYCEROL ACYLTRANSFERASE PROMOTER REGIONS FOR GENE EXPRESSION IN YEAST 有权YARROWIA DIACYLGLYCEROL ACYLTRANSFERASE促销区域用于基因表达在YEAST公开(公告)号：US20120252079A1
公开(公告)日：2012-10-04
申请号：US13435246
申请日：2012-03-30
Applicant: Quinn Qun Zhu , Zhixiong Xue
Inventor： Quinn Qun Zhu , Zhixiong Xue
IPC: C12P7/64 , C12N15/113 , C12N1/19
CPC classification number: C12N15/815 , C12N9/1029 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12Y203/0102
Abstract: Promoter regions associated with the Yarrowia lipolytica diacylglycerol acyltransferase 2 (dgat2) gene are disclosed and have been found to be particularly effective for the expression of heterologous genes in yeast. These promoter regions will be useful for driving high-level expression of genes involved in the production of omega-3 and omega-6 fatty acids.
Abstract translation: 公开了与解脂耶氏酵母双酰基甘油酰基转移酶2（dgat2）基因相关的启动子区域，并且已经发现其对酵母中异源基因的表达特别有效。 这些启动子区域可用于驱动涉及ω-3和ω-6脂肪酸生产的基因的高水平表达。
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