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    Droplet Actuator Devices and Methods Employing Magnetic Beads
    14.
    发明申请
    Droplet Actuator Devices and Methods Employing Magnetic Beads 审中-公开
    使用磁珠的液滴致动器装置和方法

    公开(公告)号：US20150314293A1

    公开(公告)日：2015-11-05

    申请号：US14719731

    申请日：2015-05-22

    Applicant: Advanced Liquid Logic, Inc. ,  Duke University

    Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

    IPC: B01L3/00 ,  G01N27/447

    CPC classification number: G01N33/54326 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0427 ,  B01L2400/043 ,  C12M25/01 ,  C12M25/16 ,  G01N27/44717 ,  G01N27/44786 ,  G01N33/54386 ,  G01N35/0098 ,  G01N2035/1046

    Abstract: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged for conducting droplet operations on a surface; (ii) a starting droplet comprising: (1) one or more magnetically responsive beads; (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone; (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet substantially lacking the magnetically responsive beads.

    Abstract translation: 提供与磁响应珠接触并具有减少的物质量的液滴的方法。 该方法通常包括以下步骤：（a）提供液滴致动器，其包括：（i）包括布置用于在表面上进行液滴操作的电极的基板; （ii）起始液滴，包括：（1）一个或多个磁响应珠; （2）物质的起始量; 和（3）起始体积; （b）将一个或多个磁响应珠磁性地固定在距离目标液滴分裂区一定距离的位置; （c）进行一个或多个液滴操作，其包括液滴分割操作，所述液滴分割操作被选择以分开所述组合的液滴以产生一组液滴，所述液滴包括：（i）包含基本上所有所述一个或多个磁响应珠粒并且具有减少量 物质相对于起始浓度; 和（ii）基本上不含磁响应珠的液滴。

    BIOTINIDASE ASSAYS
    15.
    发明申请
    BIOTINIDASE ASSAYS 审中-公开
    生物测定测定

    公开(公告)号：US20150111237A1

    公开(公告)日：2015-04-23

    申请号：US14399051

    申请日：2013-05-07

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Carrie A. Graham ,  Allen E. Eckhardt

    IPC: C12Q1/34

    CPC classification number: C12Q1/34 ,  G01N33/542 ,  G01N33/582

    Abstract: The invention relates to methods for enzymatic detection of biotinidase activity. In certain aspects, the invention provides methods for bench-based enzymatic detection of biotinidase activity. In other aspects, the invention provides methods for bench-based enzymatic detection in droplets in oil. The droplet-based methods may be performed in an automated manner using digital microfluidics technology on a droplet actuator device. The enzymatic assays for biotinidase activity may be used for newborn testing for biotinidase deficiency, and may be combined with other droplet-based enzymatic assays in a panel of tests for newborn testing.

    Abstract translation: 本发明涉及酶检测生物素酶活性的方法。 在某些方面，本发明提供了用于基于酶的酶检测生物素酶活性的方法。 在其它方面，本发明提供了用于油中液滴的基于酶的酶检测方法。 基于液滴的方法可以使用液滴致动器装置上的数字微流体技术以自动方式进行。 用于生物素酶活性的酶测定可用于生物素酶缺陷的新生儿测试，并且可以在新生儿测试的一组测试中与其他基于液滴的酶测定组合。

    Glucose-6-phosphate dehydrogenase assays
    17.
    发明授权
    Glucose-6-phosphate dehydrogenase assays 有权

    公开(公告)号：US10731199B2

    公开(公告)日：2020-08-04

    申请号：US14359177

    申请日：2012-11-20

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Carrie A. Graham ,  Allen E. Eckhardt

    IPC: C12Q1/32

    Abstract: Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.

    Analysis of DNA
    18.
    发明申请
    Analysis of DNA 审中-公开
    DNA分析

    公开(公告)号：US20160068901A1

    公开(公告)日：2016-03-10

    申请号：US14888137

    申请日：2014-05-01

    Applicant: ADVANCED LIQUID LOGIC, INC.

    Inventor： Allen E. Eckhardt ,  Michael G. Pollack ,  David S. Cohen

    IPC: C12Q1/68

    CPC classification number: C12Q1/6869 ,  B01L7/52 ,  C12Q1/6844 ,  C40B20/00 ,  C12Q2533/101 ,  C12Q2565/301

    Abstract: The invention provides pyrosequencing-based methods of analyzing and synthesizing DNA, including methods of DNA error correction, determining DNA size distribution, screening for nucleotide repeat disorders such as fragile X syndrome, determining size distribution and bias in a DNA library, and determining pyrosequencing read length. The methods include on-bench protocols as well as droplet-based protocols that may be conducted on a droplet actuator.

    Abstract translation: 本发明提供了基于焦磷酸测序的DNA分析和合成方法，包括DNA错误校正方法，确定DNA大小分布，筛选核苷酸重复序列如脆性X综合征，确定DNA文库中的大小分布和偏差，以及确定焦磷酸测序读数 长度。 这些方法包括工作台协议以及可以在液滴致动器上进行的基于液滴的协议。

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