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公开(公告)号：US20230046866A1
公开(公告)日：2023-02-16
申请号：US17962989
申请日：2022-10-10
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Uichong B. Yi , Vijay Srinivasan
Abstract: Methods of improving accuracy of droplet metering using at least one on-actuator reservoir as the fluid input. In some embodiments, the on-actuator reservoir that is used for metering droplets includes a loading port, a liquid storage zone, a droplet metering zone, and a droplet dispensing zone. The on-actuator reservoirs are designed to prevent liquid flow-back into the loading port and to prevent liquid from flooding into the droplet operations gap in the dispensing zone.
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公开(公告)号：US20220155251A1
公开(公告)日：2022-05-19
申请号：US17649842
申请日：2022-02-03
Applicant: Advanced Liquid Logic, Inc. , Duke University
Inventor： Vamsee K. Pamula , Vijay Srinivasan , Michael G. Pollack , Richard B. Fair
IPC: G01N27/26 , B01L3/00 , B01L7/00 , G01N35/00 , B01F33/302 , B01F33/3031 , C12Q1/686 , C12Q1/6869 , G01N27/447 , G01N27/327 , G01N27/416
Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
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公开(公告)号：US10799892B2
公开(公告)日：2020-10-13
申请号：US16176329
申请日：2018-10-31
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Uichong B. Yi , Vijay Srinivasan
Abstract: The present invention is directed to methods of improving accuracy of droplet metering using at least one on-actuator reservoir as the fluid input. In some embodiments, the on-actuator reservoir that is used for metering droplets includes a loading port, a liquid storage zone, a droplet metering zone, and a droplet dispensing zone. The on-actuator reservoirs are designed to prevent liquid flow-back into the loading port and to prevent liquid from flooding into the droplet operations gap in the dispensing zone.
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公开(公告)号：US20190086399A1
公开(公告)日：2019-03-21
申请号：US16191270
申请日：2018-11-14
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vamsee K. Pamula , Ramakrishna Sista , Vijay Srinivasan , Michael G. Pollack , Allen E. Eckhardt
IPC: G01N33/543 , B01F13/08 , G01N1/38
CPC classification number: G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: Provided herein are methods of splitting droplets containing magnetically responsive beads in a droplet actuator. A droplet actuator having a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at the droplet operations electrodes, is provided. The magnetically responsive beads in the droplet are immobilized using the magnetic field and the plurality of droplet operations electrodes are used to split the droplet into first and second droplets while the magnetically responsive beads remain substantially immobilized.
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公开(公告)号：US10124351B2
公开(公告)日：2018-11-13
申请号：US14911191
申请日：2014-08-13
Applicant: Advanced Liquid Logic, Inc.
Inventor： Uichong B. Yi , Vijay Srinivasan
Abstract: The present invention is directed to methods of improving accuracy of droplet metering using at least one on-actuator reservoir as the fluid input. In some embodiments, the on-actuator reservoir that is used for metering droplets includes a loading port, a liquid storage zone, a droplet metering zone, and a droplet dispensing zone. The on-actuator reservoirs are designed to prevent liquid flow-back into the loading port and to prevent liquid from flooding into the droplet operations gap in the dispensing zone.
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Patent Agency Ranking
6.发明申请公开(公告)号：US20170003282A1
公开(公告)日：2017-01-05
申请号：US15266693
申请日：2016-09-15
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vamsee K. Pamula , Ramakrishna Sista , Vijay Srinivasan , Michael G. Pollack , Allen Eckhardt
IPC: G01N33/543 , B01F13/00 , B01F13/08 , B01L3/00
CPC classification number: G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
Abstract translation: 本发明提供了一种在液滴致动器中的液滴内循环磁响应珠的方法。 本发明还提供了分裂液滴的方法。 在一个实施例中，本发明利用具有顶部和底部基底的液滴致动器，分别存在于顶部和底部基底附近的多个磁场，其中至少一个磁场可选择性地改变，并且多个液滴 沿着顶表面和底表面中的至少一个定位的操作电极。 液滴位于顶表面和底表面之间，并且至少一个磁场被选择性地改变。
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公开(公告)号：US20160370317A9
公开(公告)日：2016-12-22
申请号：US14498418
申请日：2014-09-26
Applicant: Advanced Liquid Logic, Inc.
Inventor： Arjun Sudarsan , Vijay Srinivasan , Vamsee K. Pamula , Michael G. Pollack , Ramakrishna Sista
IPC: G01N27/447
CPC classification number: G01N27/44756 , B01F13/0071 , B01F13/0076 , B01L3/502707 , B01L3/502792 , B01L2300/0816 , B01L2300/0819 , B01L2400/0427 , B01L2400/0688 , B01L2400/086 , B29C59/02 , B41J2/1601 , B81B7/0061 , B81B2201/058 , B81B2201/13 , F04B17/00 , F04B19/006
Abstract: The invention provides droplet actuators with droplet operations surfaces for manipulating droplets, e.g., by conducting droplet operations. The droplet operations surfaces are typically exposed to a droplet operations gap. One or more regions of a droplet operation surface may include patterned topographic features. The invention also provides a droplet actuator in which one or both gap-facing droplet operations surfaces is formed using a removable film. The removable film may, in various embodiments, also include other components ordinarily associated with the droplet actuator substrate, such as the dielectric layer and the electrodes. Further, the invention provides droplet actuator devices and methods for coupling and/or sealing substrates of a droplet actuator, such as techniques for self-aligning assembly of droplet actuator substrates. The invention provides droplet actuators and methods of disassembling the droplet actuator in order to provide access for cleaning and/or recycling of droplet actuator surfaces.
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公开(公告)号：US09494498B2
公开(公告)日：2016-11-15
申请号：US14978935
申请日：2015-12-22
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vamsee K. Pamula , Ramakrishna Sista , Vijay Srinivasan , Michael G. Pollack , Allen Eckhardt
IPC: B01L3/00 , B01L3/02 , G01N1/10 , G01N15/06 , G01N33/00 , G01N33/48 , G01N1/38 , B01F11/00 , B01F13/00 , G01N33/543 , B01L7/00 , G01N35/10
CPC classification number: G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
Abstract translation: 本发明提供了一种在液滴致动器中的液滴内循环磁响应珠的方法。 本发明还提供了分裂液滴的方法。 在一个实施例中，本发明利用具有顶部和底部基底的液滴致动器，分别存在于顶部和底部基底附近的多个磁场，其中至少一个磁场可选择性地改变，并且多个液滴 沿着顶表面和底表面中的至少一个定位的操作电极。 液滴位于顶表面和底表面之间，并且至少一个磁场被选择性地改变。
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公开(公告)号：US09395329B2
公开(公告)日：2016-07-19
申请号：US14805532
申请日：2015-07-22
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan
IPC: G01N1/18 , G01N35/10 , G01N27/447 , G01N15/00
CPC classification number: G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a method of providing a droplet comprising a single cell is provided, wherein the method includes providing a droplet comprising a suspension of cells on an electrowetting droplet actuator surrounded by an oil medium, dispensing from the droplet to provide a dispensed droplet, determining whether the dispensed droplet on the electrowetting droplet actuator comprises a single cell, and sorting the dispensed droplet on the electrowetting droplet actuator based on the results of the determining step using electrowetting-mediated droplet operations.
Abstract translation: 本发明涉及基于液滴的颗粒分选。 根据一个实施例，提供了一种提供包括单个电池的液滴的方法，其中所述方法包括提供液滴，其包含由油介质包围的电润湿液滴致动器上的悬浮液，从液滴分配以提供分配的液滴 确定电润湿液滴致动器上的分配液滴是否包括单个电池，并且基于使用电润湿介导的液滴操作的确定步骤的结果，将分配的液滴分配在电润湿液滴致动器上。
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公开(公告)号：US20160116438A1
公开(公告)日：2016-04-28
申请号：US14895788
申请日：2014-06-13
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Vamsee K. Pamula , Vijay Srinivasan
IPC: G01N27/447 , B01L3/00
CPC classification number: G01N27/44791 , B01J19/0093 , B01J2219/0084 , B01J2219/00853 , B01L3/502784 , B01L3/502792 , B01L2200/0673 , B01L2200/0684 , B01L2300/0816 , B01L2300/165 , B01L2300/1827 , B01L2400/0427 , B01L2400/043 , G01N27/44704
Abstract: The invention provides a droplet actuator designed to provide reliable electrical connections to droplets to reduce or eliminate gas bubble formation during droplet operations. The invention also provides methods and systems for reducing or eliminating the formation of bubbles in a droplet during droplet operations.
Abstract translation: 本发明提供了一种液滴致动器，其被设计成在液滴操作期间提供与液滴的可靠电连接以减少或消除气泡形成。 本发明还提供了用于在液滴操作期间减少或消除液滴中的气泡形成的方法和系统。
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