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公开(公告)号：US10585090B2
公开(公告)日：2020-03-10
申请号：US16132175
申请日：2018-09-14
IPC分类号： G01N15/06 , G01N33/00 , G01N33/48 , G01N35/00 , G01N1/00 , G01N1/10 , B01L3/00 , G01N33/543 , B01F13/00 , B03C1/02 , B03C1/28 , B03C1/30 , B03C5/00 , C12N15/10 , G01N33/561 , B01F11/00 , B01L7/00 , G01N35/10
摘要： Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.
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公开(公告)号：US09630180B2
公开(公告)日：2017-04-25
申请号：US14590470
申请日：2015-01-06
发明人： Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Zhishan Hua , Arjun Sudarsan , Philip Y. Paik
CPC分类号： B01L3/52 , B01F13/0071 , B01F13/0076 , B01L3/0241 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/143 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/0427 , B01L2400/06 , B01L2400/0688 , F04B19/006
摘要： A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration may include one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.
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公开(公告)号：US09476856B2
公开(公告)日：2016-10-25
申请号：US13890715
申请日：2013-05-09
IPC分类号： G01N33/543 , G01N27/72 , G01N27/74
CPC分类号： G01N27/72 , G01N27/745 , G01N33/54326
摘要： A method of detecting a target analyte in a sample, including executing electrowetting-mediated droplet operations and thereby: combining one or more immunoassay reagent droplets comprising magnetically-responsive beads having affinity for the target analyte with one or more sample droplets potentially comprising the target analyte to yield a first combined droplet; beginning with the combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a first washed droplet comprising the washed magnetically responsive beads; and combining the droplet comprising the washed magnetically responsive beads with a droplet comprising a reporter molecule having affinity for target analyte captured on the magnetically-responsive beads to yield a second combined droplet; beginning with the second combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a second washed droplet comprising the washed magnetically responsive beads; detecting a signal from the second washed droplet which corresponds to the presence, absence and/or quantity of the analyte in the sample.
摘要翻译： 一种检测样品中的目标分析物的方法，包括执行电润湿介导的液滴操作，从而：将包含对靶分析物具有亲和性的磁响应珠粒的一种或多种免疫测定试剂液滴与潜在地包含靶分析物的一个或多个样品液 以产生第一组合液滴; 从组合的液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，以产生包含洗涤的磁响应珠的第一洗涤液滴; 以及将包含洗涤的磁响应珠粒的液滴与包含对在磁响应珠上捕获的靶分析物具有亲和力的报道分子的液滴组合以产生第二组合液滴; 从第二组合液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，产生包含洗涤的磁响应珠的第二洗涤液滴; 检测来自第二洗涤液滴的信号，其对应于样品中分析物的存在，不存在和/或数量。
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公开(公告)号：US20160216231A1
公开(公告)日：2016-07-28
申请号：US15087020
申请日：2016-03-31
IPC分类号： G01N27/447 , G01N33/543 , G01N15/10 , C12Q1/68
CPC分类号： G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
摘要： The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.
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公开(公告)号：US09358551B2
公开(公告)日：2016-06-07
申请号：US14928096
申请日：2015-10-30
CPC分类号： B03C7/026 , B01L3/50273 , B01L3/502761 , B01L2200/0647 , B01L2300/0887 , B01L2400/0424 , B01L2400/0427 , B03C1/01 , B03C1/288 , B03C5/00 , B03C5/005 , B03C5/026 , B03C2201/26 , G01N33/54326
摘要： The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.
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公开(公告)号：US20150330943A1
公开(公告)日：2015-11-19
申请号：US14805532
申请日：2015-07-22
IPC分类号： G01N27/447
CPC分类号： G01N27/447 , B01L3/502761 , B01L3/502792 , B01L2200/0668 , B01L2200/0673 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2400/0427 , C12Q1/6806 , G01N15/1031 , G01N27/44717 , G01N27/44721 , G01N33/543 , G01N35/10 , G01N2015/0065 , G01N2015/0088 , G01N2015/1081 , G01N2035/1034 , Y10T436/11 , Y10T436/118339 , Y10T436/25 , Y10T436/2575
摘要： The present invention relates to droplet-based particle sorting. According to one embodiment, a method of providing a droplet comprising a single cell is provided, wherein the method includes providing a droplet comprising a suspension of cells on an electrowetting droplet actuator surrounded by an oil medium, dispensing from the droplet to provide a dispensed droplet, determining whether the dispensed droplet on the electrowetting droplet actuator comprises a single cell, and sorting the dispensed droplet on the electrowetting droplet actuator based on the results of the determining step using electrowetting-mediated droplet operations.
摘要翻译： 本发明涉及基于液滴的颗粒分选。 根据一个实施例，提供了一种提供包括单个电池的液滴的方法，其中所述方法包括提供液滴，其包含由油介质包围的电润湿液滴致动器上的悬浮液，从液滴分配以提供分配的液滴 确定电润湿液滴致动器上的分配液滴是否包括单个电池，并且基于使用电润湿介导的液滴操作的确定步骤的结果，将分配的液滴分配在电润湿液滴致动器上。
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公开(公告)号：US09081007B2
公开(公告)日：2015-07-14
申请号：US14466193
申请日：2014-08-22
IPC分类号： G01N15/06 , G01N33/00 , G01N33/48 , G01N33/561 , B01F11/00 , B01F13/00 , B01L3/00 , B03C1/02 , B03C1/30 , B03C5/00 , B01L7/00 , G01N35/10
CPC分类号： G01N33/54333 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B03C1/02 , B03C1/288 , B03C1/30 , B03C5/005 , B03C2201/18 , B03C2201/26 , C12N15/1013 , G01N33/561 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： The present invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
摘要翻译： 本发明涉及在液滴致动器上的珠孵化和洗涤。 提供了用于将用一抗标记的磁响应珠，样品（即分析物）和二次报告基因抗体孵育在磁体上，磁铁上和从磁体上离开并完全离开磁体的方法。 还提供了使用形状辅助的液滴合并来洗涤磁响应珠的方法。 还提供了用于形状介导的分离，输送和分配含有磁响应珠的样品液滴的方法。 本发明的装置和方法提供了免疫测定中分析物的快速结果和最佳检测的时间。
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公开(公告)号：US20230128722A1
公开(公告)日：2023-04-27
申请号：US18063874
申请日：2022-12-09
IPC分类号： G01N33/543 , B01L3/00 , B03C1/02 , B03C1/28 , B03C1/30 , B03C5/00 , C12N15/10 , G01N33/561 , B01F31/65 , B01F33/302 , B01F33/3031
摘要： Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.
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公开(公告)号：US20220155251A1
公开(公告)日：2022-05-19
申请号：US17649842
申请日：2022-02-03
IPC分类号： G01N27/26 , B01L3/00 , B01L7/00 , G01N35/00 , B01F33/302 , B01F33/3031 , C12Q1/686 , C12Q1/6869 , G01N27/447 , G01N27/327 , G01N27/416
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
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公开(公告)号：US20190086399A1
公开(公告)日：2019-03-21
申请号：US16191270
申请日：2018-11-14
发明人： Vamsee K. Pamula , Ramakrishna Sista , Vijay Srinivasan , Michael G. Pollack , Allen E. Eckhardt
IPC分类号： G01N33/543 , B01F13/08 , G01N1/38
CPC分类号： G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： Provided herein are methods of splitting droplets containing magnetically responsive beads in a droplet actuator. A droplet actuator having a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at the droplet operations electrodes, is provided. The magnetically responsive beads in the droplet are immobilized using the magnetic field and the plurality of droplet operations electrodes are used to split the droplet into first and second droplets while the magnetically responsive beads remain substantially immobilized.
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