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公开(公告)号：US20170130258A1
公开(公告)日：2017-05-11
申请号：US15261469
申请日：2016-09-09
Applicant: Agilent Technologies, Inc.
Inventor： Nicholas M. Sampas
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/6851 , C12Q1/6874 , C12Q2537/143 , C12Q2563/159
Abstract: This present disclosure provides methods and compositions for multiplex amplification, e.g., PCR, of DNA samples compartmentalized on the surface of an array. In certain aspects, the method includes providing a solid substrate having features of immobilized forward and reverse primer pair constructs therein for amplifying a target polynucleotide sequence, contacting the plurality of features with a corresponding nucleic acid sample, and incubating the contacted features under conditions to allow release of the forward and reverse primer constructs and amplification of the desire target polynucleotide. The release of the primer constructs from the substrate can be done prior to or during the incubating step.
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公开(公告)号：US20160369266A1
公开(公告)日：2016-12-22
申请号：US14745174
申请日：2015-06-19
Applicant: Agilent Technologies, Inc.
Inventor： Alexander Belyaev , Nicholas M. Sampas , Alicia Scheffer-Wong
IPC: C12N15/10
CPC classification number: C12N15/1065 , C12Q2521/327 , C12Q2563/179
Abstract: The present disclosure provides methods for barcoding a plurality of DNA samples using a microarray of barcode-containing transposase complexes. In some embodiments, the DNA samples and transposase complexes are present in aqueous droplets on the surfaces of opposing substrates, which allows a single DNA sample droplet to be combined with a single transposase-complex droplet. The barcoded DNA in the combined droplets can be used for any number of purposes, including as templates for amplification and sequencing.
Abstract translation: 本公开提供了使用含条形码的转座酶复合物的微阵列条形码编码多个DNA样品的方法。 在一些实施方案中，DNA样品和转座酶复合物存在于相对底物表面上的水性液滴中，这允许单个DNA样品液滴与单个转座酶复合物液滴组合。 组合液滴中的条形码DNA可用于任何数量的目的，包括用于扩增和测序的模板。
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公开(公告)号：US20240183763A1
公开(公告)日：2024-06-06
申请号：US18443049
申请日：2024-02-15
Applicant: Agilent Technologies, Inc.
Inventor： Kyle Schleifer , Kristin Briana Bernick , Adrienne Mccampbell , Nicholas M. Sampas , Victor Lim
CPC classification number: G01N1/30 , G01N1/06 , G01N1/286 , G01N35/00594 , G06T7/0012 , G01N2001/068 , G01N2001/2873 , G06T2207/10064 , G06T2207/10152 , G06T2207/30024
Abstract: The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or location of the tissue in the embedded sample.
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公开(公告)号：US20220120646A1
公开(公告)日：2022-04-21
申请号：US17564447
申请日：2021-12-29
Applicant: AGILENT TECHNOLOGIES, INC.
Inventor： Kyle Schleifer , Kristin Briana Bernick , Adrienne Mccampbell , Nicholas M. Sampas , Victor Lim
Abstract: The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or location of the tissue in the embedded sample.
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公开(公告)号：US20210108995A1
公开(公告)日：2021-04-15
申请号：US17129209
申请日：2020-12-21
Applicant: AGILENT TECHNOLOGIES, INC.
Inventor： Kyle Schleifer , Kristin Briana Bernick , Adrienne Mccampbell , Nicholas M. Sampas , Victor Lim
Abstract: The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or location of the tissue in the embedded sample.
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Patent Agency Ranking
公开(公告)号：US20170283864A1
公开(公告)日：2017-10-05
申请号：US15407998
申请日：2017-01-17
Applicant: AGILENT TECHNOLOGIES, INC.
Inventor： Robert A. Ach , Nicholas M. Sampas , Brian Jon Peter
IPC: C12Q1/68
CPC classification number: C12Q1/6855 , C12Q1/6806 , C12Q1/6848 , C12Q1/6869 , C12Q1/6874 , C12Q2521/507 , C12Q2565/514
Abstract: Described herein, among other things, is an adapter comprising a population of first oligonucleotides, a second oligonucleotide and a third oligonucleotide, wherein the first oligonucleotides, the second oligonucleotide and the third oligonucleotide are hybridized together to produce a complex that comprises: (i) a first end comprising a transposase recognition sequence, (ii) a central single-stranded region of variable sequence and (iii) a second end comprising sequences that are non-complementary. A method, as well as a kit for practicing the method, are also provided.
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公开(公告)号：US10914658B2
公开(公告)日：2021-02-09
申请号：US16868715
申请日：2020-05-07
Applicant: Agilent Technologies, Inc.
Inventor： Kyle Schleifer , Kristin Briana Bernick , Adrienne Mccampbell , Nicholas M. Sampas , Victor Lim
Abstract: The present disclosure is directed to an improved method for distinguishing tissue from an embedding medium, such as paraffin in a formalin-fixed paraffin-embedded sample. The method involves the use of fluorescence of naturally-occurring species in tissue to determine the location of the tissue in the embedded sample. An embedded sample is generally excited by light of a selected wavelength, and the fluorescence emission at an emitted wavelength is used to locate the boundary or location of the tissue in the embedded sample.
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公开(公告)号：US10711269B2
公开(公告)日：2020-07-14
申请号：US15409124
申请日：2017-01-18
Applicant: Agilent Technologies, Inc.
Inventor： Brian Jon Peter , David Taussig , Bahram Arezi , Robert A. Ach , Nicholas M. Sampas
IPC: C12N15/10 , C12Q1/6806
Abstract: A method for making an asymmetrically-tagged sequencing library is provided. In some embodiments, the method may comprise: obtaining a symmetrically-tagged library of cDNA or genomic DNA fragments, hybridizing a tailed first primer to the 3′ sequence tag of the library and extending the same to produce primer extension products, and amplifying the primer extension products using a pair of tailed primers to produce asymmetrically-tagged library.
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19.发明授权公开(公告)号：US10550425B2
公开(公告)日：2020-02-04
申请号：US15351312
申请日：2016-11-14
Applicant: AGILENT TECHNOLOGIES, INC.
Inventor： Nicholas M. Sampas , Nazumi Alice Yama-Dang , Paige Anderson
IPC: C12Q1/68 , C12Q1/6832
Abstract: Provided herein, among other things, are compositions for improving signal-to-noise in hybridization to oligonucleotide arrays. In some embodiments, the composition may comprise a population of indexing oligonucleotides of formula X-Y, where the sequences of region X hybridize to an array and the sequences of region Y hybridize to distinct sites within a genomic region, transcript, set of transcripts, or complement of the same. Use of these oligonucleotides increases signal, reduces background and smoothens array hybridization analyses. Methods and kits that employ the compositions are also provided.
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公开(公告)号：US10518241B2
公开(公告)日：2019-12-31
申请号：US14684028
申请日：2015-04-10
Applicant: Agilent Technologies, Inc.
Inventor： Nicholas M. Sampas , Richard P. Tella
IPC: B01J19/00
Abstract: Provided is an emulsion comprising: (a) droplets that contain a single polymeric compound or a pre-defined mixture thereof, and (b) an immiscible liquid, wherein: (i) each of the droplets comprises multiple molecules of the compound(s) contained therein; and the droplets do not contain monomeric precursors for the polymeric compound. A method for making the emulsion is also provided.
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