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    Immunoaffinity isolation of modified peptides from complex mixtures
    14.
    发明授权
    Immunoaffinity isolation of modified peptides from complex mixtures 有权
    来自复杂混合物的修饰肽的免疫亲和分离

    公开(公告)号：US07300753B2

    公开(公告)日：2007-11-27

    申请号：US10777893

    申请日：2004-02-12

    申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    IPC分类号： C12Q1/00 ,  C12Q1/37 ,  C12P21/06 ,  G01N33/543

    CPC分类号： G01N33/6872 ,  C07K14/47 ,  C07K16/44 ,  C12Q1/25 ,  C12Q1/485 ,  G01N33/6842

    摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS—MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

    摘要翻译： 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱法（MS），串联质谱（MS-MS）和/或MS 3分析表征步骤（c）中分离的修饰肽 或（e）利用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰的肽的亲本蛋白质 肽。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。

    Immunoaffinity isolation of modified peptides from complex mixtures
    15.
    发明授权
    Immunoaffinity isolation of modified peptides from complex mixtures 有权

    公开(公告)号：US07198896B2

    公开(公告)日：2007-04-03

    申请号：US10175486

    申请日：2002-06-19

    申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    IPC分类号： C12Q1/00 ,  C12Q1/37 ,  C12P21/06 ,  G01N33/543

    CPC分类号： C07K16/44

    摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS—MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

    Elastically expandable positioning device
    16.
    发明授权
    Elastically expandable positioning device 失效
    弹性可扩展定位装置

    公开(公告)号：US06698992B2

    公开(公告)日：2004-03-02

    申请号：US10234513

    申请日：2002-09-05

    申请人： Kung Chris Wu ,  Torben J. Ulander ,  Chawshiang Wu ,  John Rush

    发明人： Kung Chris Wu ,  Torben J. Ulander ,  Chawshiang Wu ,  John Rush

    IPC分类号： B65F302

    CPC分类号： H01L21/67775 ,  H01L21/68 ,  Y10S414/139

    摘要： An elastically expandable positioning device for positioning a cover of a unified pod is disclosed, which has a central fastener having one end protruded on the interface plate and an axial input hole opened at another end, and at least one radial distributing hole equally opened at its circular surface; a ring-shaped inflatable body made from an expandable elastic material; a supporting ring encircled around the central bolt and positioned above the distributing hole, for supporting the inflatable body, the supporting ring having at least one radial inflating hole equally positioned corresponding to the radial distributing hole of the central bolt; and an input fluid source connected to the axial input hole of the central bolt, for providing an input fluid.

    摘要翻译： 公开了一种用于定位统一舱盖的可弹性扩张的定位装置，其具有中心紧固件，该中心紧固件具有在接口板上突出的一端和在另一端开口的轴向输入孔，以及在其上同等开口的至少一个径向分配孔 圆形表面 由可膨胀弹性材料制成的环形可充气体; 围绕中心螺栓环绕并位于分配孔上方的支撑环，用于支撑可充气体，支撑环具有至少一个相应于中心螺栓的径向分布孔等同定位的径向充气孔; 以及连接到中心螺栓的轴向输入孔的输入流体源，用于提供输入流体。

    Immunoaffinity isolation of modified peptides from complex mixtures
    17.
    发明申请
    Immunoaffinity isolation of modified peptides from complex mixtures 审中-公开
    来自复杂混合物的修饰肽的免疫亲和分离

    公开(公告)号：US20120244594A9

    公开(公告)日：2012-09-27

    申请号：US11823775

    申请日：2007-06-28

    申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    IPC分类号： C12N11/00 ,  G01N33/566 ,  C07K1/14 ,  C07K16/18 ,  B01D11/02 ,  C07K16/40 ,  C07K1/22 ,  H01J49/26 ,  G01N30/72 ,  C12S3/14 ,  C07K16/00

    CPC分类号： C07K16/18 ,  B01D15/3809 ,  B01D15/3828 ,  C07K1/22 ,  C07K16/44 ,  G01N30/7233 ,  G01N33/6842 ,  G01N33/6848 ,  G01N33/6854 ,  G01N2030/8831 ,  G01N2440/14

    摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

    摘要翻译： 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱（MS），串联质谱（MS-MS）和/或MS3分析表征步骤（c）中分离的修饰肽，或（e） 利用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰肽的亲本蛋白。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。

    Device for selecting a thread in a textile machine and textile machine fitted with said device
    18.
    发明授权
    Device for selecting a thread in a textile machine and textile machine fitted with said device 失效
    用于在纺织机器中选择线的装置和装配有所述装置的纺织机

    公开(公告)号：US06279618B1

    公开(公告)日：2001-08-28

    申请号：US09600265

    申请日：2000-10-16

    申请人： Francisco Speich ,  John Rush

    发明人： Francisco Speich ,  John Rush

    IPC分类号： D03C320

    CPC分类号： D03C3/20 ,  D04B27/32

    摘要： The apparatus comprises at least one movable member (1) for the reception of the thread, a coupling arrangement (2) and a winding (3) which produces a magnetic field and is intended to produce different polarities in the coupling arrangement through which the magnetic flux passes and to bring the coupling arrangement into engagement with the member. The member (1) consists of magnetic or non-magnetic material and the coupling arrangement (2) of ferromagnetic material. The apparatus ensures a reliable coupling at high rhythm.

    摘要翻译： 该装置包括用于接收螺纹的至少一​​个可动构件（1），耦合装置（2）和产生磁场的绕组（3），并且用于在耦合装置中产生不同的极性 助焊剂通过并使联接装置与构件接合。 构件（1）由磁性或非磁性材料以及铁磁材料的耦合装置（2）组成。该设备确保了高节奏下的可靠耦合。

    Immunoaffinity isolation of modified peptides from complex mixtures
    19.
    发明申请
    Immunoaffinity isolation of modified peptides from complex mixtures 有权
    来自复杂混合物的修饰肽的免疫亲和分离

    公开(公告)号：US20050003450A1

    公开(公告)日：2005-01-06

    申请号：US10777893

    申请日：2004-02-12

    申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael Comb ,  Yi Tan

    发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael Comb ,  Yi Tan

    IPC分类号： C12P21/06 ,  C07K1/22 ,  C07K14/47 ,  C07K16/18 ,  C07K16/44 ,  C12P21/08 ,  C12Q1/25 ,  C12Q1/48 ,  G01N33/68 ,  G01N33/53

    CPC分类号： G01N33/6872 ,  C07K14/47 ,  C07K16/44 ,  C12Q1/25 ,  C12Q1/485 ,  G01N33/6842

    摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

    摘要翻译： 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱（MS），串联质谱（MS-MS）和/或MS 3分析表征步骤（c）中分离的修饰肽，或步骤 （e）使用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰肽的亲本蛋白。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。

    Immunoaffinity isolation of modified peptides from complex mixtures
    20.
    发明申请
    Immunoaffinity isolation of modified peptides from complex mixtures 审中-公开
    来自复杂混合物的修饰肽的免疫亲和分离

    公开(公告)号：US20110053242A1

    公开(公告)日：2011-03-03

    申请号：US11823775

    申请日：2007-06-28

    申请人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    发明人： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J. Comb ,  Yi Tan

    IPC分类号： C12N11/00 ,  C12S3/14 ,  G01N33/566 ,  C07K1/14 ,  C07K16/18 ,  C07K16/00 ,  C07K16/40 ,  C07K1/22 ,  H01J49/26 ,  G01N30/72 ,  B01D11/02

    CPC分类号： C07K16/18 ,  B01D15/3809 ,  B01D15/3828 ,  C07K1/22 ,  C07K16/44 ,  G01N30/7233 ,  G01N33/6842 ,  G01N33/6848 ,  G01N33/6854 ,  G01N2030/8831 ,  G01N2440/14

    摘要： The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

    摘要翻译： 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱（MS），串联质谱（MS-MS）和/或MS3分析表征步骤（c）中分离的修饰肽，或（e） 利用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰肽的亲本蛋白。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。