公开(公告)号：US20100242936A1

公开(公告)日：2010-09-30

申请号：US12415026

申请日：2009-03-31

Applicant: James Richard Zurlo ,  Kevin Paul Konkle ,  Andrew May

Inventor： James Richard Zurlo ,  Kevin Paul Konkle ,  Andrew May

IPC: F02D41/00 ,  F02M25/07

CPC classification number: F02D41/0052 ,  F02D41/187 ,  F02D2200/0411 ,  Y02T10/47

Abstract: In controlling an engine, an amount of an intake charge provided, during operation of the engine, to a combustion chamber of the engine is determined. The intake charge includes an air component, a fuel component and a diluent component. An amount of the air component of the intake charge is determined. An amount of the diluent component of the intake charge is determined utilizing the amount of the intake charge, the amount of the air component and, in some instances, the amount of the fuel component. An amount of a diluent supplied to the intake charge is adjusted based at least in part on the determined amount of diluent component of the intake charge.

Abstract translation: 在控制发动机时，确定在发动机运转期间向发动机的燃烧室提供的进气量。 进气装置包括空气部件，燃料部件和稀释剂部件。 确定进气量的空气分量的量。 使用进气量的量，空气成分的量以及在某些情况下燃料成分的量来确定进气量的稀释剂成分的量。 至少部分地基于确定的进料量的稀释剂组分的量来调节供应到进气装置的稀释剂的量。

公开(公告)号：US20080223721A1

公开(公告)日：2008-09-18

申请号：US12018138

申请日：2008-01-22

Applicant: David S. Cohen ,  Jing Wang ,  Andrew May ,  Robert C. Jones ,  Hany Nassef

Inventor： David S. Cohen ,  Jing Wang ,  Andrew May ,  Robert C. Jones ,  Hany Nassef

IPC: G01N27/26

CPC classification number: B01L3/502738 ,  B01L3/5025 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0638 ,  B01L2400/0655 ,  B33Y80/00 ,  F16K99/0001 ,  F16K99/0026 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  Y10T137/0318 ,  Y10T137/85938

Abstract: New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.

Abstract translation: 新的高密度微流体装置和方法提供流体体积的精确计量和计量体积的有效混合。 将第一溶液引入与反应室流体连通的流动通道的段中。 第二溶液流过该段，使得第一溶液移入反应室，并且第二溶液的体积进入该室。 然后可以分离室，并且可以启动和/或检测室内的反应。 遗传分析的高通量方法可以比以前更高的精度进行。

公开(公告)号：US20050075800A1

公开(公告)日：2005-04-07

申请号：US10664196

申请日：2003-09-17

Applicant: Thomas Batzinger ,  Andrew May ,  Peter Allison ,  Carl Lester

Inventor： Thomas Batzinger ,  Andrew May ,  Peter Allison ,  Carl Lester

IPC: G01B5/28 ,  G01B7/06 ,  G01B7/28 ,  G01N27/20

CPC classification number: G01B7/281

Abstract: A system and method for monitoring defects in a structure are provided. The system includes a power supply for supplying an electric current to a monitoring area of the structure and a reference; a measurement circuit for measuring a potential drop across at least two contact points of the monitoring area and at least two contact points of the reference; and a processor adapted to determine a ratio of the monitoring area potential drop to the reference potential drop indicative of a percentage change in a thickness of the structure. The method includes the steps of supplying the current to the monitoring area and the reference; measuring a first potential drop across the monitoring area and the reference; and determining the ratio indicative of the percentage change in the thickness of the structure.

公开(公告)号：US09103825B2

公开(公告)日：2015-08-11

申请号：US12764819

申请日：2010-04-21

Applicant: Hany Ramez Nassef ,  Hou-Pu Chou ,  Michael Lucero ,  Andrew May ,  Kathy Yokobata

Inventor： Hany Ramez Nassef ,  Hou-Pu Chou ,  Michael Lucero ,  Andrew May ,  Kathy Yokobata

IPC: B01L3/00 ,  G01N33/543 ,  F16K99/00 ,  G01N33/53

CPC classification number: G01N33/54366 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00389 ,  B01J2219/00398 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0655 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0051 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0084 ,  G01N33/5302

Abstract: A microfluidic device adapted to perform many simultaneous binding assays including but not limited to immunological experiments, such as ELISA assays, with minimal cross-talk between primary and secondary antibodies.

Abstract translation: 微流体装置适于执行许多同时结合测定，包括但不限于免疫学实验，例如ELISA测定，初级和第二抗体之间的最小串扰。

公开(公告)号：US20140227691A1

公开(公告)日：2014-08-14

申请号：US13977622

申请日：2011-05-16

Applicant: Andrew May ,  Alain Mir ,  Ramesh Ramakrishnan ,  Bernhard G. Zimmermann

Inventor： Andrew May ,  Alain Mir ,  Ramesh Ramakrishnan ,  Bernhard G. Zimmermann

IPC: C12P19/34 ,  C12Q1/68 ,  C07H21/00

CPC classification number: C12P19/34 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2600/118 ,  C12Q2565/113 ,  C12Q2527/125 ,  C12Q2525/204 ,  C12Q2525/307 ,  C12Q2525/15 ,  C12Q2521/507

Abstract: The present invention provides methods for selectively enriching a biological sample for short nucleic acids, such as fetal DNA in a maternal sample or apoptic DNA in a biological sample from a cancer patient and for subsequently analyzing the short nucleic acids for genotype, mutation, and/or aneuploidy.

Abstract translation: 本发明提供了用于选择性富集来自癌症患者的生物样品中的母体样品中的短核酸例如胎儿DNA的生物样品或随机分析用于基因型，突变和/ 或非整倍体。

公开(公告)号：US08691509B2

公开(公告)日：2014-04-08

申请号：US12753703

申请日：2010-04-02

Applicant: Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6806 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: In certain embodiments, the invention provides amplification methods in which nucleotide tag(s) and a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

Abstract translation: 在某些实施方案中，本发明提供其中将核苷酸标签和条形码核苷酸序列添加到靶核苷酸序列的扩增方法。 在其它实施例中，本发明提供一种包括多个第一输入线和多个第二输入线的微流体装置。 微流体装置还包括多组第一腔室和多组第二腔室。 每组第一腔室与多个第一输入管线中的一个流体连通。 每组第二腔室与多个第二输入管线中的一个流体连通。 微流体装置还包括与多个第二输入线的第一部分流体连通的多个第一泵元件和与多个第二输入线的第二部分流体连通的多个第二泵元件。

公开(公告)号：US08551787B2

公开(公告)日：2013-10-08

申请号：US12804568

申请日：2010-07-23

Applicant: Andrew May

Inventor： Andrew May

IPC: B01F5/00

CPC classification number: B01L3/502738 ,  B01F13/0059 ,  B01F15/0201 ,  B01F15/04 ,  B01F15/0429 ,  B01J19/0093 ,  B01J2219/0086 ,  B01J2219/00862 ,  B01J2219/00864 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00907 ,  B01J2219/0095 ,  B01L3/5025 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0655 ,  B33Y80/00 ,  Y10T137/0318 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides microfluidic devices and methods for carrying out sequential binary reactions.

Abstract translation: 本发明提供了用于进行顺序二元反应的微流体装置和方法。

公开(公告)号：US20120305087A1

公开(公告)日：2012-12-06

申请号：US13439390

申请日：2012-04-04

Applicant: David S. Cohen ,  Jing Wang ,  Andrew May ,  Robert C. Jones ,  Hany Nassef

Inventor： David S. Cohen ,  Jing Wang ,  Andrew May ,  Robert C. Jones ,  Hany Nassef

IPC: B01F13/00

CPC classification number: B01L3/502738 ,  B01L3/5025 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0638 ,  B01L2400/0655 ,  B33Y80/00 ,  F16K99/0001 ,  F16K99/0026 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0078 ,  F16K2099/008 ,  F16K2099/0084 ,  Y10T137/0318 ,  Y10T137/85938

Abstract: New high density microfluidic devices and methods provide precise metering of fluid volumes and efficient mixing of the metered volumes. A first solution is introduced into a segment of a flow channel in fluidic communication with a reaction chamber. A second solution is flowed through the segment so that the first solution is displaced into the reaction chamber, and a volume of the second solution enters the chamber. The chamber can then be isolated and reactions within the chamber can be initiated and/or detected. High throughput methods of genetic analysis can be carried out with greater accuracy than previously available.

Abstract translation: 新的高密度微流体装置和方法提供流体体积的精确计量和计量体积的有效混合。 将第一溶液引入与反应室流体连通的流动通道的段中。 第二溶液流过该段，使得第一溶液移入反应室，并且第二溶液的体积进入该室。 然后可以分离室，并且可以启动和/或检测室内的反应。 遗传分析的高通量方法可以比以前更高的精度进行。

公开(公告)号：US20120021523A1

公开(公告)日：2012-01-26

申请号：US13168041

申请日：2011-06-24

Applicant: Brian Fowler ,  Andrew May

Inventor： Brian Fowler ,  Andrew May

IPC: G01N23/20

CPC classification number: G01N23/20025

Abstract: An integrated fluidic circuit includes a substrate layer and a first structure coupled to the substrate layer and including a plurality of channels. The first structure is configured to provide for flow of one or more materials through the plurality of channels. The integrated fluidic circuit also includes a second structure coupled to the substrate layer. The second structure includes a plurality of control channels configured to receive an actuation pressure. The integrated fluidic circuit is characterized by a thickness of less than 1.5 mm.

Abstract translation: 集成流体回路包括衬底层和耦合到衬底层并且包括多个通道的第一结构。 第一结构被配置为提供通过多个通道的一种或多种材料的流动。 集成流体回路还包括耦合到衬底层的第二结构。 第二结构包括被配置为接收致动压力的多个控制通道。 集成流体回路的特征在于厚度小于1.5mm。

公开(公告)号：US20090147918A1

公开(公告)日：2009-06-11

申请号：US12118185

申请日：2008-05-09

Applicant: Brian Fowler ,  Andrew May

Inventor： Brian Fowler ,  Andrew May

IPC: G01N23/207

CPC classification number: G01N23/20025

Abstract: An integrated fluidic circuit includes a substrate layer and a first structure coupled to the substrate layer and including a plurality of channels. The first structure is configured to provide for flow of one or more materials through the plurality of channels. The integrated fluidic circuit also includes a second structure coupled to the substrate layer. The second structure includes a plurality of control channels configured to receive an actuation pressure. The integrated fluidic circuit is characterized by a thickness of less than 1.5 mm.

Abstract translation: 集成流体回路包括衬底层和耦合到衬底层并且包括多个通道的第一结构。 第一结构被配置为提供通过多个通道的一种或多种材料的流动。 集成流体回路还包括耦合到衬底层的第二结构。 第二结构包括被配置为接收致动压力的多个控制通道。 集成流体回路的特征在于厚度小于1.5mm。