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    SELECTIVE TAGGING OF SHORT NUCLEIC ACID FRAGMENTS AND SELECTIVE PROTECTION OF TARGET SEQUENCES FROM DEGRADATION
    3.
    发明申请
    SELECTIVE TAGGING OF SHORT NUCLEIC ACID FRAGMENTS AND SELECTIVE PROTECTION OF TARGET SEQUENCES FROM DEGRADATION 审中-公开
    选择性标记短核酸片段和选择性保护目标序列从降解

    公开(公告)号：US20100285537A1

    公开(公告)日：2010-11-11

    申请号：US12752974

    申请日：2010-04-01

    Applicant: Bernhard G. Zimmermann

    Inventor： Bernhard G. Zimmermann

    IPC: C12P19/34 ,  C02F3/34

    CPC classification number: C12N15/1093 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/1072 ,  C12Q1/6846 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2537/159

    Abstract: Methods are provided for selective tagging of short nucleic acids comprising a short target nucleotide sequence over longer nucleic acids comprising the same target nucleotide sequence. The methods can involve performing one or two cycles of amplification of a sample comprising long nucleic acids and short nucleic acids, each comprising the same target nucleotide sequence with at least two target-specific primers or primer pairs under suitable annealing conditions, wherein the primer pairs comprise: an inner primer or primer pair that can amplify the target nucleotide sequence on long and short nucleic acids (wherein each inner primer comprises a 5′ nucleotide tag; and an outer primer or primer pair that amplifies the target nucleotide sequence on long nucleic acids, but not on short nucleic acids); whereby the amplification after a second cycle produces at least one tagged target nucleotide sequence that comprises two nucleotide tags, one from each inner primer, with the target nucleotide sequence located between the nucleotide tags.

    Abstract translation: 提供了用于在包含相同靶核苷酸序列的较长核酸上选择性标记包含短目标核苷酸序列的短核酸的方法。 所述方法可以包括在合适的退火条件下进行包含长核酸和短核酸的样品的一个或两个循环的扩增，每个循环包含相同的靶核苷酸序列与至少两个靶特异性引物或引物对，其中引物对 包括：可以扩增长和短核酸上的靶核苷酸序列的内部引物或引物对（其中每个内引物包含5'核苷酸标签;以及扩增长核酸上的靶核苷酸序列的外引物或引物对 ，但不在短核酸上）; 由此在第二周期之后的扩增产生至少一个标记的靶核苷酸序列，其包含两个核苷酸标签，每个内引物中的一个，靶核苷酸序列位于核苷酸标签之间。

    Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags
    6.
    发明授权
    Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags 有权
    通过使用核苷酸标签检测多个样品中的多个靶核酸的方法

    公开(公告)号：US08697363B2

    公开(公告)日：2014-04-15

    申请号：US12548132

    申请日：2009-08-26

    Applicant: Alain Mir ,  Ramesh Ramakrishnan ,  Marc Unger ,  Bernhard G. Zimmermann

    Inventor： Alain Mir ,  Ramesh Ramakrishnan ,  Marc Unger ,  Bernhard G. Zimmermann

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12N15/1065 ,  C12Q1/6853 ,  Y10T436/143333 ,  C12Q2537/143 ,  C12Q2525/161

    Abstract: The present invention provides assay methods that increase the number of samples and/or target nucleic acids that can be analyzed in a single assay. In certain embodiments, an assay method entails separately subjecting S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence including a sample-specific nucleotide tag and a target nucleotide sequence. In some embodiments, an assay method entails separately subjecting S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence including a first nucleotide tag linked to a target nucleotide sequence, which is linked to a second nucleotide tag. In either case, the tagged target nucleotide sequences from the S samples can be mixed to form an assay mixture and subsequently assayed.

    Abstract translation: 本发明提供了增加可以在单次测定中分析的样品和/或靶核酸数量的测定方法。 在某些实施方案中，测定方法需要分别使S样品经受产生一组T标记的靶核苷酸序列的编码反应，每个标记的靶核苷酸序列包括样品特异性核苷酸标签和靶核苷酸序列。 在一些实施方案中，测定方法需要分别使S样品经受产生一组T标记的靶核苷酸序列的编码反应，每个标记的靶核苷酸序列包括与靶核苷酸序列连接的第一个核苷酸标签，其连接到第二个 核苷酸标签。 在任一情况下，可以将来自S样品的标记的靶核苷酸序列混合以形成测定混合物并随后测定。

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