公开(公告)号：US20240317820A1

公开(公告)日：2024-09-26

申请号：US18572974

申请日：2022-06-24

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Christopher Sauer ,  Mathis Appelbaum ,  Stefan Jenewein ,  Tobias Klein ,  Thomas Schweder

IPC: C07K14/32 ,  C12N9/54 ,  C12N15/75

CPC classification number: C07K14/32 ,  C12N9/54 ,  C12N15/75

Abstract: The present invention relates to a Bacillus host cell for increased production of biological compounds. Specifically, the invention relates to a Bacillus host with genetic modifications that lead to decreased production of components of biofilm and increased levels of phosphorylated De-gll. Said Bacillus host comprises at least one gene expression cassette with a polynucleotide encoding at least one polypeptide of interest, operably linked to a promoter for the production of compounds. The present invention further relates to a method for increased production of at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.

公开(公告)号：US20240294894A1

公开(公告)日：2024-09-05

申请号：US18572942

申请日：2022-06-24

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Mathis Appelbaum ,  Christopher Sauer ,  Stefan Janewein

IPC: C12N9/54 ,  C12N15/75

CPC classification number: C12N9/54 ,  C12N15/75 ,  C12Y304/21062 ,  C12N2800/101

Abstract: The present invention relates to a Bacillus host cell for increased production of biological compounds. Specifically, the invention relates to a Bacillus host with genetic modifications in the remA and/or remB gene. The present invention further relates to a method for increased production of at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.

公开(公告)号：US12065652B2

公开(公告)日：2024-08-20

申请号：US16631999

申请日：2018-07-11

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Bogdan Tokovenko

IPC: C12N15/70 ,  C12N15/74

CPC classification number: C12N15/70 ,  C12N15/74 ,  C12N2800/101 ,  C12N2800/70 ,  C12N2830/005 ,  C12N2830/34 ,  C12N2830/55

Abstract: The present invention is directed to a nucleic acid construct comprising a polynucleotide operably linked to one or more control sequence that directs the expression of the polynucleotide in a host cell, wherein at least one control sequence comprises a promoter sequence of an operon comprising a secA gene or a functional fragment or functional variant thereof and wherein said promoter sequence is heterologous to the polynucleotide. In a further embodiment, the invention is directed to an expression vector and a host cell comprising the nucleic acid construct comprising the promoter sequence described herein and a method of expressing a polynucleotide in a host cell.

公开(公告)号：US11066653B2

公开(公告)日：2021-07-20

申请号：US16631994

申请日：2018-07-11

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Stefan Seemayer

IPC: C12N15/75 ,  C12N9/10

Abstract: The present invention is directed to a producing a DNA methyltransferase in a recombinant host cell, wherein the DNA methyltransferase methylates DNA resulting in a DNA containing 5-methylcytosine within the recognition sequence GCNGC and wherein the DNA methyltransferase comprises less than (35) amino acid residues between amino acid residue (72) and amino acid residue (106) according to the numbering of SEQ ID NO: 33. Furthermore, the present invention is directed to the use of such DNA methyltransferase for the production of bacterial transformants comprising the steps of (a) introducing into a first bacterial host cell a polynucleotide comprising a polynucleotide sequence encoding the DNA methyltransferase to produce a methylated DNA and (b) transferring the methylated DNA from the first bacterial host cell into a second bacterial host cell, wherein the second bacterial host cell comprises a restriction endonuclease able to degrade the DNA but unable to degrade the methylated DNA.

公开(公告)号：US10676728B2

公开(公告)日：2020-06-09

申请号：US16062289

申请日：2016-12-08

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Stefan Jenewein ,  Stefan Handtke ,  Birgit Voigt ,  Thomas Schweder ,  Michael Hecker

IPC: C12N9/54 ,  C12N9/00 ,  C12N9/64 ,  C12N15/113 ,  C12N15/52 ,  C12N9/24 ,  C12N9/08 ,  C12N9/26 ,  C12N9/42

Abstract: The present invention generally relates to the field of fermentation technology and microorganisms useful for such fermentations. The invention also relates to materials including nucleic acids and proteins useful for altering fermentation characteristics of microorganisms, and to microorganisms comprising such nucleic acids and/or proteins. In particular, the invention relates to materials for conferring, modifying or reducing microbial stress resistance.

公开(公告)号：US20240060110A1

公开(公告)日：2024-02-22

申请号：US18030700

申请日：2021-10-06

Applicant: BASF SE

Inventor： Stefan Jenewein ,  Max Fabian Felle ,  Christopher Sauer ,  Paul Igor Costea

IPC: C12P21/02 ,  C12N9/20 ,  C12N9/16

CPC classification number: C12P21/02 ,  C12Y301/01001 ,  C12N9/20 ,  C12Y301/02002 ,  C12N9/16

Abstract: The present invention provides a modified Bacillus cell for the production of a compound of interest, wherein the Bacillus cell comprises a genetic modification that leads to decreased production of enzymes with lipase and/or esterase activity. The use of compounds of interest produced with said improved Bacillus host cells reduces the formation of malodour and/or off-flavour when used for applications such as washing or cleaning or as feed or food additives.

公开(公告)号：US11788094B2

公开(公告)日：2023-10-17

申请号：US16062291

申请日：2016-12-08

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Stefan Jenewein ,  Kerstin Hage ,  Holger Hartmann

IPC: C12N15/75 ,  C12N15/63 ,  C12N9/08 ,  C12P1/04

CPC classification number: C12N15/75 ,  C12N9/0065 ,  C12N15/63 ,  C12P1/04 ,  C12Y111/01006

Abstract: The present invention generally relates to the field of fermentation technology and microorganisms useful for such fermentations. The invention also relates to materials including nucleic acids and proteins useful for altering fermentation characteristics of microorganisms, and to microorganisms comprising such nucleic acids and/or proteins.

公开(公告)号：US20220186234A1

公开(公告)日：2022-06-16

申请号：US17432203

申请日：2020-02-18

Applicant: BASF SE

Inventor： Andreas Daub ,  Aydin Golabgir Anbarani ,  Tobias Klein ,  Stephan Freyer ,  Thomas Kaeding ,  Max Fabian Felle ,  Paul Igor Costea

IPC: C12N15/75 ,  C12N1/20 ,  C12P17/16 ,  C12N9/54

Abstract: The present invention is directed to an industrial fermentation process for cultivating a Bacillus cell in a chemically defined fermentation medium and a method for producing a protein of interest comprising the steps of providing a chemically defined fermentation medium, inoculating the fermentation medium with a Bacillus cell comprising a gene encoding a protein of interest, cultivating the Bacillus cell in the fermentation medium under conditions conductive for the growth of the Bacillus cell and the expression of the protein of interest, wherein the cultivation of the Bacillus cell comprises the addition of one or more feed solutions comprising one or more chemically defined carbon sources and one or more trace element ions to the fermentation medium.

公开(公告)号：US20200165581A1

公开(公告)日：2020-05-28

申请号：US16631994

申请日：2018-07-11

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Stefan Seemayer

IPC: C12N9/10 ,  C12N15/75

Abstract: The present invention is directed to a producing a DNA methyltransferase in a recombinant host cell, wherein the DNA methyltransferase methylates DNA resulting in a DNA containing 5-methylcytosine within the recognition sequence GCNGC and wherein the DNA methyltransferase comprises less than (35) amino acid residues between amino acid residue (72) and amino acid residue (106) according to the numbering of SEQ ID NO: 33. Furthermore, the present invention is directed to the use of such DNA methyltransferase for the production of bacterial transformants comprising the steps of (a) introducing into a first bacterial host cell a polynucleotide comprising a polynucleotide sequence encoding the DNA methyltransferase to produce a methylated DNA and (b) transferring the methylated DNA from the first bacterial host cell into a second bacterial host cell, wherein the second bacterial host cell comprises a restriction endonuclease able to degrade the DNA but unable to degrade the methylated DNA.

公开(公告)号：US20170022531A1

公开(公告)日：2017-01-26

申请号：US15104446

申请日：2014-12-12

Applicant: BASF SE

Inventor： Stefan Jenewein ,  Max Fabian Felle ,  Jan Reyelt

IPC: C12P21/00 ,  C12N15/70 ,  C07K14/245

CPC classification number: C12P21/00 ,  C07K14/245 ,  C12N15/64 ,  C12N15/68 ,  C12N15/70

Abstract: A process for production of a protein of interest in a microbial host organism comprising the steps of:a) constructing a vector comprising a gene for the protein of interest and a functional frr gene and no antibiotic resistance gene,b) transforming a host organism having an inactivated chromosomal frr gene with the vector obtained in a),c) culturing the transformed host organism obtained in b) under conditions allowing the expression of the gene of interest in the host organism andd) isolating the protein of interest.

Abstract translation: d）分离感兴趣的蛋白质。