公开(公告)号：US20240060110A1

公开(公告)日：2024-02-22

申请号：US18030700

申请日：2021-10-06

Applicant: BASF SE

Inventor： Stefan Jenewein ,  Max Fabian Felle ,  Christopher Sauer ,  Paul Igor Costea

IPC: C12P21/02 ,  C12N9/20 ,  C12N9/16

CPC classification number: C12P21/02 ,  C12Y301/01001 ,  C12N9/20 ,  C12Y301/02002 ,  C12N9/16

Abstract: The present invention provides a modified Bacillus cell for the production of a compound of interest, wherein the Bacillus cell comprises a genetic modification that leads to decreased production of enzymes with lipase and/or esterase activity. The use of compounds of interest produced with said improved Bacillus host cells reduces the formation of malodour and/or off-flavour when used for applications such as washing or cleaning or as feed or food additives.

公开(公告)号：US11788094B2

公开(公告)日：2023-10-17

申请号：US16062291

申请日：2016-12-08

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Stefan Jenewein ,  Kerstin Hage ,  Holger Hartmann

IPC: C12N15/75 ,  C12N15/63 ,  C12N9/08 ,  C12P1/04

CPC classification number: C12N15/75 ,  C12N9/0065 ,  C12N15/63 ,  C12P1/04 ,  C12Y111/01006

Abstract: The present invention generally relates to the field of fermentation technology and microorganisms useful for such fermentations. The invention also relates to materials including nucleic acids and proteins useful for altering fermentation characteristics of microorganisms, and to microorganisms comprising such nucleic acids and/or proteins.

公开(公告)号：US20220267748A1

公开(公告)日：2022-08-25

申请号：US17636972

申请日：2020-08-21

Applicant: BASF SE

Inventor： Stefan Jenewein ,  Zachary David Miles ,  Priya Anand

IPC: C12N9/26 ,  C11D3/386 ,  A21D8/04

Abstract: Genetically engineered enzymes having amylase enzyme activity, compositions comprising the enzymes, and methods of making and using the enzymes. The genetically engineered amylase enzymes are useful in many different applications such as laundry detergents, dish washing detergents, and cleaning products for homes, industry, vehicle care, baking, animal feed, pulp and paper processing, starch processing, brewing, and ethanol production.

公开(公告)号：US10898739B2

公开(公告)日：2021-01-26

申请号：US16715273

申请日：2019-12-16

Applicant: BASF SE

Inventor： Nina Schneider ,  Thomas Subkowski ,  Stefan Jenewein ,  Marvin Karos ,  Claus Bollschweiler ,  Volker Wendel ,  Jianhong Qiu ,  LaTonya Kilpatrick-liverman ,  Lynette Zaidel

IPC: A61Q11/00 ,  A61K8/21 ,  A61K8/64 ,  A61K9/68 ,  A61P43/00 ,  A61P1/02

Abstract: Provided herein are oral care compositions comprising antifreeze proteins (AFPs) useful in methods of repairing or inhibiting dental erosion, promoting dental remineralization, and/or enhancing the anti-cavity effects of fluoride.

公开(公告)号：US20190201721A1

公开(公告)日：2019-07-04

申请号：US15771236

申请日：2016-10-25

Applicant: BASF SE

Inventor： Nina Schneider ,  Thomas Subkowski ,  Stefan Jenewein ,  Marvin Karos ,  Claus Bollschweiler ,  Volker Wendel ,  Thorsten Henkes ,  Sandra Facey ,  Bernhard Hauer

IPC: A61Q11/00 ,  A61K8/64

CPC classification number: A61Q11/00 ,  A61K8/21 ,  A61K8/24 ,  A61K8/27 ,  A61K8/64

Abstract: Provided herein are oral care compositions comprising HABP, which are useful in methods of repairing or inhibiting dental erosion, promoting dental remineralization, and/or enhancing the anti-cavity effects of fluoride.

公开(公告)号：US20190022429A1

公开(公告)日：2019-01-24

申请号：US15770978

申请日：2016-10-25

Applicant: BASF SE

Inventor： Nina Schneider ,  Thomas Subkowski ,  Stefan Jenewein ,  Marvin Karos ,  Claus Bollschweiler ,  Volker Wendel ,  Jianhong Qiu ,  LaTonya Kilpatrick-Liverman ,  Lynette Zaidel

IPC: A61Q11/00 ,  A61K8/21 ,  A61K8/64

CPC classification number: A61Q11/00 ,  A61K8/21 ,  A61K8/64 ,  A61K9/0058

Abstract: Provided herein are oral care compositions comprising antifreeze proteins (AFPs) useful in methods of repairing or inhibiting dental erosion, promoting dental remineralization, and/or enhancing the anti-cavity effects of fluoride.

公开(公告)号：US20170044509A1

公开(公告)日：2017-02-16

申请号：US15306232

申请日：2015-04-15

Applicant: BASF SE

Inventor： Stefan Jenewein ,  Stefan Niedermaier

IPC: C12N9/26

CPC classification number: C12N9/2414 ,  C12N9/2417 ,  C12Y302/01001

Abstract: The present invention pertains to the field of amylases, particularly alpha-amylases, and compositions comprising such amylases. Also described are means and methods for producing such amylases, and uses of amylases and amylase-comprising compositions, for example for cleaning starch-containing stains, textile desizing, starch liquefaction and saccharification, baking and brewing. The invention also discloses means for increasing amylase purity during fermentation, formulation and storage of amylase or amylase-containing formulations.

Abstract translation: 本发明涉及淀粉酶，特别是α-淀粉酶和包含这种淀粉酶的组合物领域。 还描述了生产这种淀粉酶的方法和方法，以及淀粉酶和淀粉酶组合物的用途，例如用于清洁含淀粉的污渍，纺织品脱浆，淀粉液化和糖化，烘焙和酿造。 本发明还公开了用于在发酵，配制和储存淀粉酶或含淀粉酶的制剂期间增加淀粉酶纯度的方法。

公开(公告)号：US20170022531A1

公开(公告)日：2017-01-26

申请号：US15104446

申请日：2014-12-12

Applicant: BASF SE

Inventor： Stefan Jenewein ,  Max Fabian Felle ,  Jan Reyelt

IPC: C12P21/00 ,  C12N15/70 ,  C07K14/245

CPC classification number: C12P21/00 ,  C07K14/245 ,  C12N15/64 ,  C12N15/68 ,  C12N15/70

Abstract: A process for production of a protein of interest in a microbial host organism comprising the steps of:a) constructing a vector comprising a gene for the protein of interest and a functional frr gene and no antibiotic resistance gene,b) transforming a host organism having an inactivated chromosomal frr gene with the vector obtained in a),c) culturing the transformed host organism obtained in b) under conditions allowing the expression of the gene of interest in the host organism andd) isolating the protein of interest.

Abstract translation: d）分离感兴趣的蛋白质。

公开(公告)号：US20240318188A1

公开(公告)日：2024-09-26

申请号：US18572827

申请日：2022-06-24

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Mathis Appelbaum ,  Christopher Sauer ,  Stefan Jenewein

IPC: C12N15/75 ,  C07K14/32 ,  C12N9/48

CPC classification number: C12N15/75 ,  C07K14/32 ,  C12N9/48

Abstract: The present invention relates to a Bacillus licheniformis host cell for production of biological compounds with increased purity. Specifically, the invention relates to a Bacillus licheniformis host with one or more genetic modifications selected from a) a genetic modification causing compared to an unmodified control cell an increased expression of at least one of the genes selected from the group consisting of degQ, degll, degS, degR, and phrG, b) a genetic modification causing compared to an unmodified control cell an increased autophosphorylation activity of the DegS protein, and c) a genetic modification causing compared to an unmodified control cell a reduced phosphatase activity of the DegS protein. The present invention further relates to a method for production of at least one compound of interest with increased purity based on cultivating the bacterial host cell of the present invention.

公开(公告)号：US20240317820A1

公开(公告)日：2024-09-26

申请号：US18572974

申请日：2022-06-24

Applicant: BASF SE

Inventor： Max Fabian Felle ,  Christopher Sauer ,  Mathis Appelbaum ,  Stefan Jenewein ,  Tobias Klein ,  Thomas Schweder

IPC: C07K14/32 ,  C12N9/54 ,  C12N15/75

CPC classification number: C07K14/32 ,  C12N9/54 ,  C12N15/75

Abstract: The present invention relates to a Bacillus host cell for increased production of biological compounds. Specifically, the invention relates to a Bacillus host with genetic modifications that lead to decreased production of components of biofilm and increased levels of phosphorylated De-gll. Said Bacillus host comprises at least one gene expression cassette with a polynucleotide encoding at least one polypeptide of interest, operably linked to a promoter for the production of compounds. The present invention further relates to a method for increased production of at least one polypeptide of interest based on cultivating the bacterial host cell of the present invention.