公开(公告)号：US20170174892A1

公开(公告)日：2017-06-22

申请号：US15371064

申请日：2016-12-06

Applicant: Becton, Dickinson and Company

Inventor： Robert J. Radford ,  Douglas B. Sherman ,  Jody Martin ,  James Ghadiali

IPC: C09B69/10 ,  G01N33/58

CPC classification number: C09B69/109 ,  C09B57/10 ,  C09B69/00 ,  C09B69/102 ,  C09K11/06 ,  G01N33/582 ,  G01N2458/30

Abstract: Photostable polymeric tandem dyes are provided. The photostable polymeric tandem dyes include a water soluble light harvesting multichromophore and a luminescent metal complex covalently linked to the multichromophore and in energy-receiving proximity therewith. Also provided are labelled specific binding members that include the subject dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric tandem dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.

公开(公告)号：US11460468B2

公开(公告)日：2022-10-04

申请号：US15715028

申请日：2017-09-25

Applicant: Cellular Research, Inc. ,  Becton, Dickinson and Company

Inventor： Christina Fan ,  Christina Chang ,  Eleen Shum ,  Olaf Zoellner ,  Nidhanjali Bansal ,  James Ghadiali ,  Gretchen Yinbon Lam ,  Eric Jensen ,  Jurg Rohrer ,  Brent Gaylord ,  Jody Martin ,  Jason G. Vidal

IPC: G01N33/566 ,  G16B99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68 ,  C12Q1/6804 ,  G16Z99/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/68 ,  G16B25/10 ,  G16H20/13 ,  G16H10/20

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US11099190B2

公开(公告)日：2021-08-24

申请号：US15780219

申请日：2016-12-06

Applicant: Becton, Dickinson and Company

Inventor： Jody Martin ,  Robert J. Radford ,  James Ghadiali

IPC: G01N33/58 ,  G01N33/542 ,  C07K16/44 ,  G01N33/53 ,  C09K11/06

Abstract: Fluorogenic sensors for a target analyte are provided. The fluorogenic sensors can include a water soluble light harvesting multichromophore and a fluorogenic dye covalently linked to the multichromophore and in energy-receiving proximity therewith. Aspects of the fluorogenic sensors include selective recognition of the target analyte by chemical reaction or by specific binding. In some cases, the linked fluorogenic dye includes a lactone or spiro-lactone group that is configured to chemically react with a target analyte via lactone ring-opening to produce a fluorescent dye configured for excitation by the multichromophore. Also provided are labelled specific binding members that include the subject dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject fluorogenic sensors find use are provided. Systems, devices and kits for practicing the subject methods are provided.

公开(公告)号：US10408759B2

公开(公告)日：2019-09-10

申请号：US15534771

申请日：2016-04-25

Applicant: Becton, Dickinson and Company

Inventor： James Ghadiali

IPC: G01N21/64 ,  G01N15/14 ,  G01N33/58 ,  G01N15/10

Abstract: Methods for producing a high resolution image of a sample are provided. In some embodiments, the method includes: detecting first and second sets of spatially-dependent emission signals from a sample labeled with a fluorescent polymeric dye; and producing a high resolution fluorescence image of the sample from the detected first and second sets of spatially-dependent emission signals. In some embodiments, the sample is a cell. Also provided are systems for imaging a sample that include a high resolution light microscope including a light source configured to irradiate a field of view with an excitation light; a photodetector configured to detect an emission signal: and a polarization modulator disposed in the light pathway between the light source and the photodetector; and a sample labelled with a polymeric dye and disposed in the field of view.

公开(公告)号：US20180232484A1

公开(公告)日：2018-08-16

申请号：US15886763

申请日：2018-02-01

Applicant: Becton, Dickinson and Company

Inventor： Jody Martin ,  Adam Wright ,  Paul Waterman ,  James Ghadiali

IPC: G06F19/22 ,  G06F17/30

CPC classification number: G16B30/00 ,  G06F16/2455 ,  G06F16/248 ,  G16B50/30

Abstract: Aspects of the present disclosure include systems for use in preparing an epitope tagged biomolecule reagent. A reagent preparatory apparatus for preparing the epitope tagged biomolecule reagent from an activated biomolecule and activated epitope tag is also described. Methods for communicating and receiving an epitope tagged biomolecule reagent request and preparing the subject epitope tagged biomolecule reagents are also provided.

公开(公告)号：US20170370847A1

公开(公告)日：2017-12-28

申请号：US15534771

申请日：2016-04-25

Applicant: Becton, Dickinson and Company

Inventor： James Ghadiali

IPC: G01N21/64 ,  G01N15/14 ,  G01N33/58 ,  G01N15/10

CPC classification number: G01N21/6458 ,  G01N15/1436 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6445 ,  G01N33/582 ,  G01N2015/1006 ,  G01N2015/1447 ,  G01N2021/6439 ,  G01N2201/0683

Abstract: Methods for producing a high resolution image of a sample are provided. In some embodiments, the method includes: detecting first and second sets of spatially-dependent emission signals from a sample labeled with a fluorescent polymeric dye; and producing a high resolution fluorescence image of the sample from the detected first and second sets of spatially-dependent emission signals. In some embodiments, the sample is a cell. Also provided are systems for imaging a sample that include a high resolution light microscope including a light source configured to irradiate a field of view with an excitation light; a photodetector configured to detect an emission signal: and a polarization modulator disposed in the light pathway between the light source and the photodetector; and a sample labelled with a polymeric dye and disposed in the field of view.

公开(公告)号：US20170248587A1

公开(公告)日：2017-08-31

申请号：US15509827

申请日：2015-11-11

Applicant: Becton, Dickinson and Company

Inventor： James Ghadiali ,  Jody Martin

IPC: G01N33/533 ,  G01N33/569 ,  G01N33/543 ,  G01N33/58

CPC classification number: G01N33/533 ,  G01N33/54326 ,  G01N33/56972 ,  G01N33/582

Abstract: Proteinaceous specific binding members that specifically bind to a polymeric dye are provided. Also provided are methods of using the specific binding members, e.g., in separating a polymeric dye-labeled cell from a sample, in analyte detection, etc., as described herein. Kits and systems for practicing the subject methods are also provided.