公开(公告)号：US20140124370A1

公开(公告)日：2014-05-08

申请号：US14051004

申请日：2013-10-10

Applicant: Berkeley Lights, Inc.

Inventor： Steven W. Short ,  Ming C. Wu

IPC: B03C5/00 ,  B03C5/02

CPC classification number: B03C5/005 ,  B01L3/502761 ,  B01L2400/0424 ,  B03C5/026 ,  B03C2201/26

Abstract: A microfluidic optoelectronic tweezers (OET) device can comprise dielectrophoresis (DEP) electrodes that can be activated and deactivated by controlling a beam of light directed onto photosensitive elements that are disposed in locations that are spaced apart from the DEP electrodes. The photosensitive elements can be photodiodes, which can switch the switch mechanisms that connect the DEP electrodes to a power electrode between an off state and an on state.

Abstract translation: 微流体光电镊子（OET）装置可以包括介电电泳（DEP）电极，其可以通过控制定向到设置在与DEP电极间隔开的位置的感光元件上的光束而被激活和去激活。 感光元件可以是光电二极管，其可以将连接DEP电极的开关机构切换到关闭状态和导通状态之间的功率电极。

公开(公告)号：US20140017791A1

公开(公告)日：2014-01-16

申请号：US13940424

申请日：2013-07-12

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Igor Y. Khandros ,  Gaetan L. Mathieu ,  Steven W. Short ,  Ming C. Wu

IPC: C12N13/00

CPC classification number: C12N13/00 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0454 ,  C12N15/02

Abstract: Two or more biological micro-objects can be grouped in a liquid medium in a chamber. Grouping can comprise bringing into and holding in proximity or contact the micro-objects in a group, breaching the membrane of one or more of the micro-objects in a group, subjecting one or more of the micro-objects in a group to electroporation, and/or tethering to each other the micro-objects in a group. The micro-objects in the group can then be combined into a single biological object.

Abstract translation: 两个或更多个生物微物体可以分组在室中的液体介质中。 分组可以包括进入和保持接近或接触组中的微物体，破坏组中的一个或多个微物体的膜，使一组中的一个或多个微物体进行电穿孔， 和/或彼此束缚在一组中的微物体。 然后，组中的微物体可以组合成单个生物体。

公开(公告)号：US11998914B2

公开(公告)日：2024-06-04

申请号：US17656244

申请日：2022-03-24

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M. Jimena Loureiro

IPC: B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  G01N15/14 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68

CPC classification number: B01L3/502761 ,  B03C5/005 ,  B03C5/026 ,  G01N15/1484 ,  G01N33/5023 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5052 ,  G01N33/54313 ,  G01N33/6854 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0454 ,  B03C2201/26

Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US20200230601A1

公开(公告)日：2020-07-23

申请号：US16683798

申请日：2019-11-14

Applicant: Berkeley Lights, Inc.

Inventor： Mark P. White ,  Eric D. Hobbs ,  J. Tanner Nevill ,  Daniele Malleo ,  Steven W. Short

IPC: B01L3/00 ,  G01N33/558

Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.

公开(公告)号：US10646871B2

公开(公告)日：2020-05-12

申请号：US15989549

申请日：2018-05-25

Applicant: Berkeley Lights, Inc.

Inventor： Mark P. White ,  Eric D. Hobbs ,  J. Tanner Nevill ,  Daniele Malleo ,  Steven W. Short

IPC: G01N33/558 ,  B01L3/00

Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.

公开(公告)号：US20200078788A1

公开(公告)日：2020-03-12

申请号：US16509166

申请日：2019-07-11

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Mark P. White ,  M. Jimena Loureiro

IPC: B01L3/00 ,  B03C5/02 ,  B03C5/00 ,  G01N15/14 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68

Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.

公开(公告)号：US20180126380A1

公开(公告)日：2018-05-10

申请号：US15637159

申请日：2017-06-29

Applicant: Berkeley Lights, Inc.

Inventor： Igor Y. Khandros ,  Daniele Malleo ,  J. Tanner Nevill ,  Steven W. Short ,  Ming C. Wu

IPC: B01L3/00 ,  B03C5/02 ,  B03C5/00

CPC classification number: B01L3/502761 ,  B01L3/502792 ,  B01L2200/0647 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26

Abstract: A structure for providing a boundary for a chamber in a microfluidic apparatus can comprise dielectrophoresis (DEP) configurations each having an outer surface and electrowetting (EW) configurations each having an electrowetting surface. The DEP configurations can facilitate generating net DEP forces with respect to the outer surfaces of the DEP configurations to move micro-objects on the outer surfaces, and the EW configurations can facilitate changing wetting properties of the electrowetting surfaces to move droplets of liquid medium on the electrowetting surfaces.

公开(公告)号：US09895699B2

公开(公告)日：2018-02-20

申请号：US15207210

申请日：2016-07-11

Applicant: Berkeley Lights, Inc.

Inventor： Steven W. Short ,  Ming C. Wu

IPC: B03C5/00 ,  B03C5/02 ,  B01L3/00

CPC classification number: B03C5/005 ,  B01L3/502761 ,  B01L2400/0424 ,  B03C5/026 ,  B03C2201/26

Abstract: A microfluidic optoelectronic tweezers (OET) device can comprise dielectrophoresis (DEP) electrodes that can be activated and deactivated by controlling a beam of light directed onto photosensitive elements that are disposed in locations that are spaced apart from the DEP electrodes. The photosensitive elements can be photodiodes, which can switch the switch mechanisms that connect the DEP electrodes to a power electrode between an off state and an on state.

公开(公告)号：US20170021366A1

公开(公告)日：2017-01-26

申请号：US15105849

申请日：2014-12-18

Applicant: Berkeley Lights, Inc.

Inventor： Kevin T. Chapman ,  Eric D. Hobbs ,  Steven W. Short ,  Mark P. White ,  Daniele Malleo

IPC: B03C5/00 ,  B03C11/00 ,  B01L3/00 ,  B03C5/02

CPC classification number: B03C5/005 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2200/14 ,  B01L2400/0424 ,  B03C5/026 ,  B03C11/00 ,  B03C2201/26

Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.

Abstract translation: 可以在微流体装置中选择各种生物细胞，并将其移动到装置中的隔离笔中。 然后，细胞可以在笔中裂解，从而释放核酸物质，其可被笔中的一个或多个捕获物捕获。 然后可以从笔中取出捕获的核酸材料的捕获物体。 捕获对象可以包括唯一的标识符，允许每个捕获对象与由对象捕获的核酸材料源自的单个细胞相关联。

公开(公告)号：US20150151298A1

公开(公告)日：2015-06-04

申请号：US14520568

申请日：2014-10-22

Applicant: Berkeley Lights, Inc.

Inventor： Eric D. Hobbs ,  Mark P. White ,  J. Tanner Nevill ,  Daniele Malleo ,  Steven W. Short

IPC: B01L3/00 ,  G01N33/558

CPC classification number: B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0424 ,  B01L2400/0433 ,  B01L2400/0454 ,  G01N33/558 ,  G01N2469/20

Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.

Abstract translation: 微流体装置可以包括至少一个流体连接到未被吹扫的区域的扫掠区域。 扫掠区域和未被扫描的区域之间的流体连接可以实现扩散，但是基本上不会在扫掠区域和未被扫描区域之间的介质流动。 可以在这种微流体装置中测定生物微生物产生感兴趣分析物的能力。 可以选择装载到微流体装置中的样品材料中的生物微物体用于特定特征并且被置于未被扫描的区域中。 样品材料然后可以从扫掠区域流出，并且测定材料流入扫掠区域。 扫描区域中介质的流动基本上不影响未被扫描的区域中的生物微物体，但是由生物微物体产生的任何目标分析物可以从未被扫描的区域扩散到扫描区域，其中分析物可与 测定材料以产生局部可检测的反应。 可以分析任何这样的检测到的反应，以确定生物微物体（如果有的话）是感兴趣的分析物的生产者。