公开(公告)号：US11965001B2

公开(公告)日：2024-04-23

申请号：US16927590

申请日：2020-07-13

Applicant: California Institute of Technology

Inventor： Dan I. Piraner ,  Mikhail Shapiro

IPC: C07K14/255

CPC classification number: C07K14/255 ,  C07K2319/73

Abstract: Provided herein are thermomer monomer, thermomer dimers, thermomer monomeric constructs, thermomer dimeric complexes, and related gene expression cassette vectors, cells, surfaces devices, compositions methods and systems, that provide a thermobioswitch suitable to control location and/or binding of cargo moiety of interest in a temperature regulated manner.

公开(公告)号：US11944423B2

公开(公告)日：2024-04-02

申请号：US17097421

申请日：2020-11-13

Applicant: California Institute of Technology

Inventor： Saransh Sharma ,  Mikhail Shapiro ,  Azita Emami

IPC: A61B5/07 ,  A61B5/00 ,  A61B5/05 ,  G01B7/31 ,  G01R33/022 ,  G01R33/385

CPC classification number: A61B5/073 ,  A61B5/05 ,  A61B5/076 ,  A61B5/6861 ,  G01B7/31 ,  G01R33/022 ,  G01R33/385 ,  A61B2562/0223

Abstract: A method for in-vivo monitoring of a target internal volume of a mammal that includes: (a) placing the target internal volume proximal to a three-dimensional magnetic field generator; (b) generating first, second, and third magnetic field gradients along respective first, second, and third axes that are mutually orthogonal; (c) measuring first, second, and third magnetic fields with a three-dimensional magnetic sensor disposed in an ingestible capsule, the ingestible capsule disposed in the target internal volume; (d) with a controller in electrical communication with the three-dimensional magnetic sensor, generating a magnetic sensor output signal that encodes a measurement of the first, second, and third magnetic fields; (e) broadcasting the magnetic sensor output signal from an antenna disposed in the ingestible capsule, and (f) receiving the magnetic sensor output signal with a receiver. The received magnetic field data can be used to determine the three-dimensional position of the ingestible capsule.

公开(公告)号：US11786218B2

公开(公告)日：2023-10-17

申请号：US16736581

申请日：2020-01-07

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Daniel P. Sawyer ,  Arash Farhadi ,  Avinoam D. Bar-Zion ,  Mikhail Shapiro

IPC: A61B8/08

CPC classification number: A61B8/5207 ,  A61B8/481 ,  A61B8/5246

Abstract: The application of a step function increase in acoustic pressure during ultrasound imaging using gas vesicle contrast, along with capturing successive frames of ultrasound imaging and extracting time-series vectors for pixels of the frames, allows for improved imaging down to even the cell level. Template vectors can be used to implement signal separation of the time-series vectors to improve detection.

公开(公告)号：US11761008B2

公开(公告)日：2023-09-19

申请号：US16736683

申请日：2020-01-07

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Arash Farhadi ,  Gabrielle H Ho ,  Daniel P Sawyer ,  Mikhail Shapiro ,  Robert C. Hurt ,  Mengtong Duan

IPC: C12N15/67 ,  C07K14/195

CPC classification number: C12N15/67 ,  C07K14/195 ,  C12N2510/02

Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.

公开(公告)号：US11653899B2

公开(公告)日：2023-05-23

申请号：US16736581

申请日：2020-01-07

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Daniel P. Sawyer ,  Arash Farhadi ,  Avinoam D. Bar-Zion ,  Mikhail Shapiro

IPC: A61B8/08

CPC classification number: A61B8/5207 ,  A61B8/481 ,  A61B8/5246

Abstract: The application of a step function increase in acoustic pressure during ultrasound imaging using gas vesicle contrast, along with capturing successive frames of ultrasound imaging and extracting time-series vectors for pixels of the frames, allows for improved imaging down to even the cell level. Template vectors can be used to implement signal separation of the time-series vectors to improve detection.

公开(公告)号：US20210353439A1

公开(公告)日：2021-11-18

申请号：US17318821

申请日：2021-05-12

Applicant: California Institute of Technology ,  INSERM (Institut National de la Santé et de la Recherche Médicale) ,  CNRS-CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris

Inventor： Sumner L Norman ,  David Maresca ,  Vasileios Christopoulos ,  Mikhail Shapiro ,  Richard A. Andersen ,  Mickael Tanter ,  Charlie Demene

IPC: A61F2/70 ,  A61B8/06 ,  A61F4/00 ,  A61N1/08 ,  G16H30/40 ,  G06N20/00

Abstract: Methods and systems are provided for decoding movement intentions using functional ultrasound (fUS) imaging of the brain. In one example, decoding movement intentions include determining a memory phase of a cognitive state of the brain, the memory phase between a gaze fixation phase and movement execution phase, and determining one or more movement intentions including one or more of intended effector (e.g., hand, eye) and intended direction (e.g., right, left) according to a machine learning algorithm trained to classify one or more movement intentions simultaneously.

公开(公告)号：US12109440B2

公开(公告)日：2024-10-08

申请号：US17816373

申请日：2022-07-29

Applicant: California Institute of Technology

Inventor： Avinoam D. Bar-Zion ,  Atousa Nourmahnad ,  David Maresca ,  Mikhail Shapiro

IPC: A61N7/00

CPC classification number: A61N7/00 ,  A61N2007/0004 ,  A61N2007/0039 ,  A61N2007/0052 ,  A61N2007/0082

Abstract: The system and process of therapeutic and effective cavitation by using ultrasound to collapse gas vesicles as well as cavitate the bubbles produced from the collapsed gas vesicles. Therapeutic effect includes, but is not limited to lysing cells by cavitation. The cells expressing the gas vesicles can optionally be used as delivery cells to preform tasks such as transporting the gas vesicles into deep tissue areas, releasing compounds at the cavitation site, and more. The gas vesicles can optionally be modified to facilitate getting the bubbles near the cavitation targets by functionalizing the gas vesicles.

公开(公告)号：US11903595B2

公开(公告)日：2024-02-20

申请号：US17813177

申请日：2022-07-18

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Saransh Sharma ,  Abhinav Agarwal ,  Mikhail Shapiro ,  Azita Emami

IPC: A61B17/17 ,  G01B7/31

CPC classification number: A61B17/1707 ,  A61B17/1725 ,  G01B7/31

Abstract: A three dimensional magnetic sensor attached to a surgical nail is located based on an applied monotonic magnetic field gradient. Another three dimensional magnetic sensor locates a surgical drill. A display generates a real time image of the relative alignment of the surgical drill and of the surgical nail, allowing a surgeon to repair bone fractures.

公开(公告)号：US11457835B2

公开(公告)日：2022-10-04

申请号：US17097349

申请日：2020-11-13

Applicant: California Institute of Technology

Inventor： Saransh Sharma ,  Mikhail Shapiro ,  Azita Emami

IPC: G01R33/00 ,  A61B5/07 ,  A61B5/00 ,  A61B5/05 ,  G01B7/31 ,  G01R33/022 ,  G01R33/385

Abstract: An apparatus for producing magnetic field gradients includes (a) a first planar electromagnet coil set configured to produce a first magnetic field gradient with respect to a first axis, (b) a second planar electromagnet coil set configured to produce a second magnetic field gradient with respect to a second axis that is orthogonal to the first axis, (c) a third planar electromagnet coil set configured to produce a third magnetic field gradient with respect to a third axis that is orthogonal to the first and second axes, and (d) a controller configured to selectively provide power to the first, second, and/or third electromagnet coil sets to sequentially produce a localization magnetic field gradient with respect to each of the first, second, and third axes, at least a portion of each localization magnetic field gradient having a monotonically-varying magnetic field magnitude along a respective axis.

公开(公告)号：US20210324389A1

公开(公告)日：2021-10-21

申请号：US17230998

申请日：2021-04-15

Applicant: California Institute of Technology

Inventor： Mohamad H. Abedi ,  Mikhail Shapiro ,  Dan I. Piraner ,  Justin Lee

IPC: C12N15/63 ,  C07K14/725

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in spatiotemporal regulation of therapeutic T-cells through a combination of molecular and physical actuation. There are provided, in some embodiments, thermal bioswitches that allow T-cells to sense small changes in temperature and use them as inputs for the actuation of genetic circuits. Also disclosed herein are T cell activity sensors. Genetic circuits capable of inducing expression of a payload upon thermal stimulation and/or immune cell stimulation are provided. There are provided, in some embodiments, thermally actuated immune cells and methods of using are provided. Oscillator circuits and methods of preventing T cell exhaustion are also disclosed.