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36 条记录 (耗时 0.033 秒)

    Reagents for lysis of bacterial cells
    11.
    发明授权
    Reagents for lysis of bacterial cells 有权
    细菌细胞溶解试剂

    公开(公告)号：US09371509B2

    公开(公告)日：2016-06-21

    申请号：US12855803

    申请日：2010-08-13

    申请人： Christian Birkner ,  Herbert von der Eltz ,  Monika Soukupova

    发明人： Christian Birkner ,  Herbert von der Eltz ,  Monika Soukupova

    IPC分类号： C12N1/06

    CPC分类号： C12N1/06 ,  C12N1/20

    摘要： The invention provides a composition containing an aqueous liquid phase, bacterial cells, and an ionic compound dissolved in the liquid phase. The ionic compound is selected from the group consisting of 1-butyl-3-methyl-imidazolium-thiocyanate, 1-butyl-3-methyl-imidazolium-2(2-methoxy-ethoxy)ethylsulfate, 1-methyl-1-[4-(3-methyl-3H-imidazol-1-ium)-but-1-yl]-3H-imidazolium-di(toluylsulfate), and 1-butyl-3-methyl-imidazolium-octylsulfate. The compositions of the invention are advantageously used for preparing lysates of biological cells, particularly bacterial cells.

    摘要翻译： 本发明提供一种含有水相液相，细菌细胞和溶于液相的离子化合物的组合物。 离子化合物选自1-丁基-3-甲基 - 咪唑鎓 - 硫氰酸酯，1-丁基-3-甲基 - 咪唑鎓-2（2-甲氧基 - 乙氧基）乙基硫酸酯，1-甲基-1- [4 - （3-甲基-3H-咪唑-1-基） - 丁-1-基] -3H-咪唑鎓 - 二（甲苯基硫酸盐）和1-丁基-3-甲基 - 咪唑鎓辛基硫酸盐。 本发明的组合物有利地用于制备生物细胞，特别是细菌细胞的裂解物。

    Detection of PCR products in gel electrophoresis
    13.
    发明授权
    Detection of PCR products in gel electrophoresis 失效
    凝胶电泳检测PCR产物

    公开(公告)号：US08053213B2

    公开(公告)日：2011-11-08

    申请号：US12876320

    申请日：2010-09-07

    申请人： Christian Birkner

    发明人： Christian Birkner

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/00 ,  C07H21/02

    CPC分类号： C12Q1/686 ,  C12Q2565/125 ,  C12Q2563/173 ,  C12Q2561/113

    摘要： Disclosed is a method for analyzing nucleic acids in a sample comprising the following steps: (i) adding a DNA binding dye containing a benzothiazolium or benzoxazolium group to the sample to be analyzed, (ii) carrying out a polymerase chain reaction, (iii) applying the sample to a gel matrix, (iv) separating the nucleic acid molecules according to their size by applying a voltage and (v) excitation with light of a suitable wavelength for the optical visualization of the nucleic acid/DNA binding dye complexes.

    摘要翻译： 本发明公开了一种分析样品中的核酸的方法，包括以下步骤：（i）向待分析的样品中加入含有苯并噻唑鎓或苯并恶唑鎓基团的DNA结合染料，（ii）进行聚合酶链式反应，（iii） 将样品施加到凝胶基质上，（iv）通过施加电压和（v）用适当波长的光激发核酸/ DNA结合染料复合物的光学可视化，根据其大小分离核酸分子。

    NUCLEIC ACID ADSORPTION UNDER LOW-SALT CONDITIONS
    14.
    发明申请
    NUCLEIC ACID ADSORPTION UNDER LOW-SALT CONDITIONS 有权
    低盐条件下的核酸吸附

    公开(公告)号：US20090012285A1

    公开(公告)日：2009-01-08

    申请号：US12105381

    申请日：2008-04-18

    申请人： Christian Birkner

    发明人： Christian Birkner

    IPC分类号： C07H1/06 ,  C07H1/00 ,  C09K3/00

    CPC分类号： C12N15/1006

    摘要： Biscationic organic compounds are disclosed which promote adsorption of nucleic acids from an aqueous solution to a solid phase such as silica. Adsorption takes place under low salt conditions. Further disclosed are methods and kits suitable for nucleic acid isolation from aqueous solutions.

    摘要翻译： 揭示了一种促进核酸从水溶液吸附到固相如二氧化硅的杂质有机化合物。 吸附在低盐条件下进行。 还公开了适用于从水溶液中分离核酸的方法和试剂盒。

    Roofing membrane
    17.
    发明授权
    Roofing membrane 失效
    屋顶膜

    公开(公告)号：US06830802B2

    公开(公告)日：2004-12-14

    申请号：US10302355

    申请日：2002-11-22

    申请人： Niels Graae ,  Christian Birkner

    发明人： Niels Graae ,  Christian Birkner

    IPC分类号： C08L9500

    CPC分类号： B32B11/04 ,  B32B3/14 ,  B32B2395/00 ,  B32B2419/06 ,  D06N5/00 ,  E04D5/12 ,  E04D13/17 ,  Y10T428/24355 ,  Y10T428/24372 ,  Y10T428/24405 ,  Y10T428/2443 ,  Y10T428/31815

    摘要： A roofing membrane, includes at least one support layer and a plurality of strips arranged in spaced-apart disposition at least on one side of the support layer and made of bituminous material and have a penetration depth between about 120 and 170 at a temperature of 50° C., and a softening point between about 100° C. and 110° C. The strips of bituminous material are separated from one another by a layer of scattered material and are covered by a fusible film. The film is composed of a plurality of strips which are oriented in parallel relationship to the bituminous strips but not interconnected.

    摘要翻译： 顶盖膜包括至少一个支撑层和多个条带，其布置成间隔布置在支撑层的至少一侧上并由沥青材料制成，并且在50℃的温度下具有介于约120和170之间的穿透深度 ℃，软化点在约100℃和110℃之间。沥青材料条通过一层分散的材料彼此分离，并被可熔膜覆盖。 该膜由多个条带组成，这些条带与烟煤条平行地定向但不相互连接。

    Method and device for monitoring a pressure sensor
    18.
    发明授权
    Method and device for monitoring a pressure sensor 有权
    用于监测压力传感器的方法和装置

    公开(公告)号：US06234148B1

    公开(公告)日：2001-05-22

    申请号：US09379220

    申请日：1999-08-23

    申请人： Andreas Hartke ,  Christian Birkner

    发明人： Andreas Hartke ,  Christian Birkner

    IPC分类号： F02M700

    CPC分类号： F02D41/3863 ,  F02D41/222 ,  F02D2041/1433 ,  F02D2041/223 ,  F02D2200/0602 ,  F02D2200/0604 ,  G01L27/007

    摘要： A method for monitoring a pressure sensor, which determines a pressure in a pressure accumulator regulated by a pressure actuator, includes the steps of calculating an expected pressure value in the pressure accumulator for a given point in time based on a holding pressure preset by the pressure actuator and based on a detected rate of change in a mass balance of a medium contained in the pressure accumulator. A pressure value in the pressure accumulator is determined with the pressure sensor at the given point in time. The expected pressure value is compared with the determined pressure value and a malfunction in the pressure sensor is detected if the pressure values deviate from each other beyond a predetermined value. A device for monitoring a pressure sensor is also provided.

    摘要翻译： 一种用于监测压力传感器的方法，其确定由压力致动器调节的压力存储器中的压力，包括以下步骤：基于由压力预设的保持压力，计算给定时间点内的蓄压器中的预期压力值 并且基于检测到的包含在蓄压器中的介质的质量平衡的变化率。 在给定的时间点，用压力传感器确定蓄能器中的压力值。 将预期压力值与确定的压力值进行比较，并且如果压力值彼此偏离预定值，则检测压力传感器中的故障。 还提供了用于监测压力传感器的装置。